| Project/Area Number |
16K14622
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Sasaki Foundation (2017)
National Cancer Center Japan (2016) |
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Principal Investigator |
Yamaguchi Hideki 公益財団法人佐々木研究所, 附属研究所, 部長(移行) (10345035)
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| Research Collaborator |
SAITO Emi
OKABE Akira
KOSAKAI Ruri
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | スキルス胃癌 / 腹膜播種 / 蛍光イメージング / 腫瘍不均一性 / 癌 |
|---|
| Outline of Final Research Achievements |
Scirrhous gastric carcinoma (SGC) rapidly and diffusively infiltrates in the gastric submucosa and frequently causes peritoneal dissemination. To understand the process of SGC peritoneal dissemination, we investigated heterogeneity/clonality of peritoneal tumors by multicolor fluorescent imaging. SGC cells were clonally and fluorescently marked by RGB marking method for cell lineage tracking. RGB-marked cells were i.p. or orthotopically injected into nude mice and peritoneal tumors were analyzed by fluorescent microscopy. As a result, each of the peritoneal tumors displayed multiple colors, demonstrating that they were comprised of multiclonal and heterogenous cell populations.
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