| Project/Area Number |
16K14623
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | National Hospital Organization Nagoya Medical Center |
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Principal Investigator |
Komano Atsushi 独立行政法人国立病院機構(名古屋医療センター臨床研究センター), その他部局等, 臨床検査科長 (60356251)
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| Research Collaborator |
TAKEDA Satoshi 国立感染症研究所, 研究員
TANAKA Atsushi 大阪大学, 微生物学研究所, 助教
HOSHINO Tyuji 千葉大学, 薬学部, 准教授
OKADA Seiji 熊本大学, エイズ学センター, 教授
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | HTLV-1 / ATL / provirus / genome editing / Protein transduction / LENA / ゲノム編集 / ZFN / CRISPR/Cas9 / LTR / malignant phenotype / genome loss / Cancer biology / パッセンジャー説 |
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| Outline of Final Research Achievements |
HTLV-1 is a causative agent of adult T-cell leukemia (ATL) in humans. The role of HTLV-1 in the maintenance of malignant phenotype of ATL cells has remained clarified. We employed evolving genome editing technologies to remove the proviral genome of HTLV-1 from ATL cells to address this issue. We were able to detect a PCR signal, suggesting the proviral removal was successful. We have been trying to reproduce this finding and will analyze the biological properties of provirus-deleted ATL cells.
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