Screening of no-coding RNA that can control transcription and chromatin

• Project/Area Number: 16K14638
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Hokkaido University
• Principal Investigator: Murakami Yota 北海道大学, 理学研究院, 教授 (20260622)
• Co-Investigator(Renkei-kenkyūsha): TAKAHATA Shinya 北海道大学, 理学研究院, 助教 (50381588)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: non-coding RNA / 転写制御 / 分裂酵母 / スクリーニング / 遺伝子発現 / エピジェネティクス / 分裂区公募

Outline of Final Research Achievements

To screen non-coding RNAs that have an ability to control transcription in fission yeast, we tried to construct a screening system using structure-specific RNA binding protein MS12 that enable tether non-coding RNAs to the promoter of reporter genes. However, we gave up to use MS12, because MS2 itself shows transcription stimulation activity. We next try to utilize CRISPR-dCAS9 to tether RNAs onto the promoter. We succeeded to recruit CRISPR-dCAS9 to the target region of fission yeast genome, but its efficiency is still low. We are trying to improve the efficiency.

Research Products

• [Journal Article] H3K36 methylation state and associated silencing mechanisms. (2017)
  • Author(s): Suzuki S, Murakami Y, Takahata S.
  • Journal Title: Transcription Volume: 8 Issue: 1 Pages: 26-31
  • DOI: 10.1080/21541264.2016.1246076
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Histone H3K36 trimethylation is essential for multiple silencing mechanism in fission yeast. (2016)
  • Author(s): Shota Suzuki, Hiroaki Kato, Yutaka Suzuki, Yuji Chikashige, Yasushi Hiraoka, Hiroshi Kimura, Koji Nagao, Chikashi Obuse, Shinya Takahata and Yota Murakami
  • Journal Title: Nucleic Acids Research Volume: 44 (9) Issue: 9 Pages: 4147-4162
  • DOI: 10.1093/nar/gkw008
  • NAID: 120005819302
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions. (2016)
  • Author(s): Tange Y, Chikashige Y, Takahata S, Kawakami K, Higashi M, Mori C, Kojidani T, Hirano Y, Asakawa H, Murakami Y, Haraguchi T, Hiraoka Y.
  • Journal Title: Genes to Cells Volume: 21 Issue: 8 Pages: 812-832
  • DOI: 10.1111/gtc.12385
  • NAID: 120005906898
  • Peer Reviewed / Open Access