| Project/Area Number |
16K14638
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Genome biology
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
Murakami Yota 北海道大学, 理学研究院, 教授 (20260622)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TAKAHATA Shinya 北海道大学, 理学研究院, 助教 (50381588)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | non-coding RNA / 転写制御 / 分裂酵母 / スクリーニング / 遺伝子発現 / エピジェネティクス / 分裂区公募 |
|---|
| Outline of Final Research Achievements |
To screen non-coding RNAs that have an ability to control transcription in fission yeast, we tried to construct a screening system using structure-specific RNA binding protein MS12 that enable tether non-coding RNAs to the promoter of reporter genes. However, we gave up to use MS12, because MS2 itself shows transcription stimulation activity. We next try to utilize CRISPR-dCAS9 to tether RNAs onto the promoter. We succeeded to recruit CRISPR-dCAS9 to the target region of fission yeast genome, but its efficiency is still low. We are trying to improve the efficiency.
|
