| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
In this research program, we have constructed a novel gene structure annotation protocol using both experimental and computational analyses. In the experimental analysis, the 5′ end of cDNA was protected. Then, the digestion reaction from the 3′ end was performed using Exo3. Finally, from the 5′ end, one sample was obtained, while from the 3′ end various samples were obtained. Next, we constructed an Illumina library from these samples, but we were not successful in the preparation of samples ranging from around 1,000 bp, even using various protocols. In the computational analysis, we succeeded in constructing a novel cDNA assembly program for the abovementioned data, and the performance of the simulation data was satisfactory. We did not succeed in achieving the initial target, but we could obtain 5′ and 3′ end contigs from the same cDNA; this information is very useful for gene identification. Hence, this program can be applied to improve the accuracy of gene identification.
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