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Metabolic diversity in serpentinized ecosystem

Research Project

Project/Area Number 16K14647
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Genome biology
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

Suzuki Shino  国立研究開発法人海洋研究開発機構, 高知コア研究所, 特任主任研究員 (10557002)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsメタゲノム / メタトランスクリプトーム / 極限微生物 / 蛇紋岩化反応 / メタトランスクリプトミクス / 蛇紋岩化作用 / メタゲノミクス / 好アルカリ微生物 / 好アルカリ性菌 / 蛇紋岩水系 / 地球深部生命圏
Outline of Final Research Achievements

Serpentinization is a water-rock reaction to produce a highly alkaline fluid, and hydrogen. The MAG of the phylum NPL-UPA2, named Unc8, is about 1 Mbp and its biosynthetic properties suggest it is capable of independent growth. With regard to energy metabolism, the MAG of Unc8 encodes all enzymes for Wood-Ljungdahl(WL) pathway, a ferredoxin:NAD+ oxidoreductase (Rnf) and electron carriers for flavin-based electron bifurcation (Etf, Hdr). Furthermore, the transcriptome of Unc8 in the waters of The Cedars showed enhanced levels of gene expression in the key enzymes of theWL pathway, which indicated that the Unc8 is an acetogen. However, the MAG of Unc8 encoded no well-known hydrogenase genes, suggesting that the energy metabolism of Unc8 might be focused on CO as the carbon and energy sources for the acetate formation. Given that CO could be supplied via abiotic reaction associated with deep subsurface serpentinization, CO-associated metabolism could provide advantageous approach.

Academic Significance and Societal Importance of the Research Achievements

橄欖岩―水の反応で作られる水素は、その環境に生きる生命の重要なエネルギー源となりうる。この反応は初期地球では現在より多く起きていたこと、そして、超塩基性岩は、宇宙空間にも多く存在することなどが推定されているため、単に環境微生物学領域のみならず、アストロバイオロジーや初期生命研究にも大きく貢献するものである。現存する類似環境には、その進化の過程で最適化したエネルギー代謝を有する者が生き残っているはずである。この類似環境に生きる生命のもつ特異な代謝システムが明らかになったことは、時空間を超えた生命のハビタビリティーの理解の拡大につながるものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2018 2017 2016 Other

All Int'l Joint Research (5 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (16 results) (of which Int'l Joint Research: 7 results,  Invited: 12 results)

  • [Int'l Joint Research] Universidade de Lisboa(ポルトガル)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] J. Craig Venter Institute/University of Southern California/JPL(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] IRNAS-CSIC(スペイン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Istituto Nazionale di Geofisica(イタリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Southern California(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Linking serpentinization, hyperalkaline mineral waters and abiotic methane production in continental peridotites: an integrated hydrogeological-bio-geochemical model from the Cabe?o de Vide CH4-rich aquifer (Portugal)2018

    • Author(s)
      Marques J.M.、Etiope G.、Neves M.O.、Carreira P.M.、Rocha C.、Vance S.D.、Christensen L.、Miller A.Z.、Suzuki S.
    • Journal Title

      Applied Geochemistry

      Volume: 96 Pages: 287-301

    • DOI

      10.1016/j.apgeochem.2018.07.011

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genomic and in-situ Transcriptomic Characterization of the Candidate Phylum NPL-UPL2 From Highly Alkaline Highly Reducing Serpentinized Groundwater2018

    • Author(s)
      Suzuki Shino、Nealson Kenneth H.、Ishii Shun’ichi
    • Journal Title

      Frontiers in Microbiology

      Volume: 9 Pages: 646-649

    • DOI

      10.3389/fmicb.2018.03141

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Unusual metabolic diversity of hyperalkaliphilic microbial communities associated with subterranean serpentinization at The Cedars2017

    • Author(s)
      Suzuki Shino、Ishii Shun'ichi、Hoshino Tatsuhiko、Rietze Amanda、Tenney Aaron、Morrill Penny L、Inagaki Fumio、Kuenen J Gijs、Nealson Kenneth H
    • Journal Title

      The ISME Journal

      Volume: 11 Issue: 11 Pages: 2584-2598

    • DOI

      10.1038/ismej.2017.111

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 岩に生かされる生命たち:地球深部強アルカリ環境をすみかとする微生物の生き様に迫る2018

    • Author(s)
      鈴木志野
    • Organizer
      第61回海中海底工学フォーラム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Unusual metabolic strategies identified in a hyperalkaliphilic microbial community associated with the serpentinization2018

    • Author(s)
      Shino Suzuki
    • Organizer
      日本地球惑星科学連合2018年大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Microbial adaptive evolution to the oxic/anoxic interfaces in serpentinizing systems2018

    • Author(s)
      Shino Suzuki
    • Organizer
      日本地球惑星科学連合2018年大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Microbial adaptive evolution to the extreme geochemistry occurring at the serpentinization systems2018

    • Author(s)
      Shino Suzuki
    • Organizer
      日本微生物生態学会 第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 初期地球類似環境に見られる特異な微生物代謝システム2018

    • Author(s)
      鈴木志野
    • Organizer
      細胞を創る研究会11.0
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Extensive Loss of Methanogenic Pathway: Adaptive Strategy of Methanosarcinales to the Hyperalkaline Setting Associated with Serpentinization2018

    • Author(s)
      Shino Suzuki
    • Organizer
      Gordon Research Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Integration of cultivation and genomics unveils survival strategies of extremophilic microbial life2018

    • Author(s)
      Shino Suzuki
    • Organizer
      第91回日本細菌学会 シンポジウム
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 蛇紋岩化反応に支えられる微生物群 ~氷の衛星で生命は検出できるのか?~2018

    • Author(s)
      鈴木 志野
    • Organizer
      第三回アストロバイオロジーキャンプ
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Microbial adaptation to the extreme environment revealed by genome-centric metagenomics2017

    • Author(s)
      Shino Suzuki
    • Organizer
      第12回 国際ゲノム会議
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 極限への適応:培養と環境ゲノミクスで紐解く生命の生存戦略2017

    • Author(s)
      鈴木志野
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Hyperalkaliphiles:蛇紋岩深部流体と地球表層の境界に形成される超アルカリ性生命圏2017

    • Author(s)
      花田祐一、石井俊一、Ana Z. Miller, Jose; M. Marques、Kenneth H. Nealson、鈴木志野
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Research-status Report
  • [Presentation] 微生物ゲノム情報で紐解く蛇紋岩生命圏2017

    • Author(s)
      鈴木志野
    • Organizer
      InterRidge-Japan研究会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Microbial community associated with subterranean serpentinization2017

    • Author(s)
      Shino Suzuki
    • Organizer
      KIOST International Seminar
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Metabolic diversity of hyperalkaliphilic microbial communities associated with serpentinization at The Cedars2017

    • Author(s)
      Shino Suzuki
    • Organizer
      Goldschmidt 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Comparative Genomics Identifies Serpentinomonas Adaptation to the Calcium-rich Highly-alkaline Serpentinizing Ecosystem2016

    • Author(s)
      Shino Suzuki, Shunichi Ishii, Yuichi Hanada, Lina J. Bird, J. Gijs Kuenen, Kenneth H. Nealson
    • Organizer
      Extremophiles 2016
    • Place of Presentation
      京都大学(京都府京都市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Metabolic Diversity of Microorganisms Associated with Active Subsurface Serpentinization2016

    • Author(s)
      Suzuki Shino,Shunichi Ishii, Tatsuhiko Hoshino, Fumio Inagaki, J. Gijs Kuenen, Kenneth H. Nealson
    • Organizer
      Goldschmidt 2016
    • Place of Presentation
      パシフィコ横浜 (神奈川県横浜市)
    • Year and Date
      2016-06-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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