| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
To determine the epigenetic effects on the progression on Alzheimer’s disease (AD), the gene expression through microRNA in the brain was analyzed. All autopsied brains are systematically diagnosed with Braak staging based on the density of amyloid deposition and neurofibrillary tangles in the brain. The temporal cortex tissues were carefully dissected and stored at －80 °C until use. The expression of miR-501_3P was remarkably upregulated in the AD brains related to the pathological progression.
The overexpression of miR-501-3p in the neuroblastoma derived cultured cells (SH-SY5Y), which mimicked the miR-501-3p upregulation in the AD brains, resulted in the significant changes in the expression levels of 208 genes in which 128 genes were downregulated. Mutation in the seed sequence at the position 2 to 8 nucleotide of the miR-501_3P induced the significant alternative expression of the target mRNA in the SH-SY5Y cells.
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