• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a method for LC-MS analysis for cleared organs

Research Project

Project/Area Number 16K14653
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field System genome science
Research InstitutionThe University of Tokyo

Principal Investigator

ODE Koji  東京大学, 大学院医学系研究科(医学部), 助教 (40612122)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsプロテオミクス / 質量分析 / 組織透明化 / 蛋白質ネットワーク / 透明化組織イメージング
Outline of Final Research Achievements

We developed a method to acquire local proteomics information from the biological samples subjected to tissue clearing. During the development, we noticed that the reagents used in the tissue-clearing method called CUBIC have following three problems: 1) the reagent(s) inhibits trypsin digestion reaction, 2) it sticks to the capillary of LC-MS system, and 3) it non-specifically binds to the reverse-phase resin used for the desalting peptides. To overcome these problems, we set up a screening system for novel cleansing reagents using purified proteins and cultured cells. We succeeded to find a set of chemicals that can achieve tissue clearing by delipidation and adjust the refractive index, while the chemicals have minimum effect on the protease activity, do not stick to the LC-MS capillaries as well as reverse-phase resin.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Invited: 6 results)

  • [Journal Article] Design Principles of Phosphorylation-Dependent Timekeeping in Eukaryotic Circadian Clocks2017

    • Author(s)
      Ode Koji L.、Ueda Hiroki R.
    • Journal Title

      Cold Spring Habor Perspective in Biology

      Volume: - Issue: 8 Pages: a028357-a028357

    • DOI

      10.1101/cshperspect.a028357

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Knockout-Rescue Embryonic Stem Cell-Derived Mouse Reveals Circadian-Period Control by Quality and Quantity of CRY1.2017

    • Author(s)
      Ode KL, Ukai H, Susaki EA, Narumi R, Matsumoto K, Hara J, Koide N, Abe T, Kanemaki MT, Kiyonari H, Ueda HR.
    • Journal Title

      Mol. Cell

      Volume: 65 Issue: 1 Pages: 176-190

    • DOI

      10.1016/j.molcel.2016.11.022

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Molecular, organismal and evolutional tuning of circadian period through the multisite phosphorylation of CRY12017

    • Author(s)
      大出晃士, 上田泰己
    • Organizer
      Conbio 2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Circadian-period determination by mCRY1 degradation-dependent and -independent mechanisms2017

    • Author(s)
      大出晃士, 上田泰己
    • Organizer
      第24回日本時間生物学会学術大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Flexible time: turning of circadian period through the loop region of CRYPTOCHROME protein.2017

    • Author(s)
      大出晃士, 上田泰己
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Information processing governed by dynamic protein phosphorylation2016

    • Author(s)
      大出晃士
    • Organizer
      第54回日本生物物理学会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 非専門家のためのプロテオミクス活用作戦2016

    • Author(s)
      大出晃士
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 哺乳類概日時計周期長を制御する生化学的基盤2016

    • Author(s)
      大出晃士
    • Organizer
      第16回日本抗加齢医学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-06-10
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Using MS at the bench:the post-translational landscape of circadian clock proteins2016

    • Author(s)
      大出晃士
    • Organizer
      第64回質量分析総合討論会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府吹田市)
    • Year and Date
      2016-05-18
    • Related Report
      2016 Research-status Report
    • Invited

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi