A library preparation method for single cell genomic sequencing with long-read sequencers
| Project/Area Number |
16K14656
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | ゲノム配列決定 / １本鎖DNA / ニッキングエンドヌクレアーゼ / TACSライゲーション / ゲノム配列 / 1本鎖DNA / 1細胞 / 次世代シークエンサー |
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| Outline of Final Research Achievements |
Using nicking endonucleases, DNA fragments with different cut sites in the top and bottom strands of double-stranded DNA can be prepared. Therefore, if a highly efficient method for adaptor tagging to single-stranded DNA is available, a sequencing library of DNA fragments with differential structures between the top and bottom strands of DNA may be prepared. In this project, we tried to establish such a protocol, based on a highly efficient single-stranded DNA ligation technology developed by us. The established protocol has been applied to sequencing using short-read sequencers like that from Illumina. In the future, we expect its application in long-read sequencers like that from Oxford Nanopore Technologies.
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Report
(3 results)
Research Products
(3 results)
