| Project/Area Number |
16K14658
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | Teikyo University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ゲノム再構築 / Qβファージ / 感染性cDNA / モジュラーゲノム / 人工ウイルス / SD配列 / 遺伝子デザイン / RNAファージ / ゲノム再構成 / ウイルスゲノム |
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| Outline of Final Research Achievements |
The complementary DNA (cDNA) of the genome RNA of E. coli RNA phage Qβ was divided into the seven functional units (modules). Using the site-directed mutagenesis, unique recognition sequences for restriction enzymes were created at the both sides of the genes. The altered Qβ cDNA consisted of three gene units and four non-gene units which contained the control regions of gene expression. Furthermore, two kinds of infectious cDNAs in which the ribosome binding sequences were introduced into the upstream region of the coat gene were made. It becomes possible to reconstruct Qβ cDNA by joining the modules and to make chimera cDNAs by replacing the units with the corresponding ones from other phages. The control region of the Qβ coat gene was thought to be engaged in phage particle formation.
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