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Development of 3D-gradient cultivation method for as-yet-uncultivated microorganisms growing in a narrow range of environmental conditions

Research Project

Project/Area Number 16K14663
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Conservation of biological resources
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

KATO Shingo  国立研究開発法人海洋研究開発機構, 次世代海洋資源調査技術研究開発プロジェクトチーム, 特任研究員 (40554548)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords分離培養 / 難培養微生物 / 難培養性微生物 / 単離培養 / 応用微生物 / 生態学 / 生理学
Outline of Final Research Achievements

Isolation and cultivation of as-yet-uncultivated microorganisms are essential for basic and applied studies of microorganisms. However, most of microbial species is still as-yet-uncultivated. In the present study, I developed new cultivation methods to efficiently isolate not-yet-cultivated microorganisms. Using the methods, some novel species of iron oxidizers and ammonia oxidizers were successfully isolated or enriched in culture from natural environments. The new cultivation methods will contribute to discover and exploit novel useful microorganisms.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results)

  • [Journal Article] Long-Term Cultivation and Metagenomics Reveal Ecophysiology of Previously Uncultivated Thermophiles Involved in Biogeochemical Nitrogen Cycle2018

    • Author(s)
      Kato, S., Sakai, S., Hirai, M., Tasumi, E., Nishizawa, M., Suzuki, K. and Takai, K.
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 1 Pages: 107-110

    • DOI

      10.1264/jsme2.ME17165

    • NAID

      130006581753

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel Pelagic Iron-Oxidizing Zetaproteobacteria from the Chesapeake Bay Oxic-Anoxic Transition Zone2017

    • Author(s)
      Chiu Beverly K.、Kato Shingo、McAllister Sean M.、Field Erin K.、Chan Clara S.
    • Journal Title

      Frontiers in Microbiology

      Volume: 8 Pages: 1280-1280

    • DOI

      10.3389/fmicb.2017.01280

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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