Verification of genomic diversity required for tumorigenesis
Project/Area Number |
16K14664
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
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Research Institution | Tohoku University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
城田 松之 東北大学, 医学系研究科, 助教 (00549462)
舟山 亮 東北大学, 医学系研究科, 助教 (20452295)
細金 正樹 東北大学, 医学系研究科, 助手 (30734347)
|
Project Period (FY) |
2016-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
|
Keywords | ゲノム編集 / CRISPR/Cas9システム / 超並列シークエンス解析 / 腫瘍形成能 / Geminin / エピゲノム / 細胞周期 / ES細胞 / Cdt1 / エピゲム / 転写制御 / MLL2 |
Outline of Final Research Achievements |
CRISPR/Cas9 library was introduced to NIH3T3 cells and MDA-MD-468 cells to generate cell library which are gene disrupted. Groups of cells which was disrupted a gene by CRISPR/Cas9 system were obtained. These cells were inoculated subcutaneously on the back of immunodeficient mice to grow tumors. Even NIH3T3 cells which were introduced library failed to stable tumorigenesis as well as wild type cells. These data suggested that NIH3T3 population containing different kinds of a single gene disrupted cells did not achieve capability of tumorigenesis. With MDA-MD-462 cells, we observed cells introduced CRISPR/Cas9 library gain function of tumorigenesis compared to wild-type cells.
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Report
(2 results)
Research Products
(19 results)
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[Journal Article] Geminin is an indispensable inhibitor of Cdt1 in mouse embryonic stem cells.2017
Author(s)
Hosogane, M., Bosu, L., Fukumoto, E., Yamada, H., Sato, S., Nakayama, K.
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Journal Title
Genes Cells
Volume: 印刷中
Issue: 4
Pages: 360-375
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A Bach2-Cebp gene regulatory network for the commitment of multipotent hematopoietic progenitors.2017
Author(s)
Itoh-Nakadai, A., Matsumoto, M., Kato, H., Sasaki, J., Uehara, Y., Sato, Y., Ebina-Shibuya, R., Morooka, M., Funayama, R., Nakayama, K., Ochiai, K., Muto, A. and Igarashi, K.
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Journal Title
Cell Reports
Volume: 18
Issue: 10
Pages: 2401-2414
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A large-scale targeted proteomics assay resource based on an in vitro human proteome.2017
Author(s)
Matsumoto M, Matsuzaki F, Oshikawa K, Goshima N, Mori M, Kawamura Y, Ogawa K, Fukuda E, Nakatsumi H, Natsume T, Fukui K, Horimoto K, Nagashima T, Funayama R, Nakayama K, Nakayama KI.
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Journal Title
Nat Methods
Volume: 14
Issue: 3
Pages: 251-258
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Regulatory signatures of liver regeneration distilled by integrative analysis of mRNA, histone methylation, and proteomics.2017
Author(s)
Sato, Y., Katoh, Y., Matsumoto, M., Sato, M., Ebina, M., Itoh-Nakadai, A., Funayama, R., Nakayama, K., Unno, M., Igarashi, K.
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Journal Title
J.Biol.Chem.
Volume: 印刷中
Issue: 19
Pages: 8019-8037
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Exome sequencing deciphers a germline MET mutation in familial epidermal growth factor receptor-mutant lung cancer.2017
Author(s)
Tode N, Kikuchi T, Sakakibara T, Hirano T, Inoue A, Ohkouchi S, Tamada T, Okazaki T, Koarai A, Sugiura H, Niihori T, Aoki Y, Nakayama K, Matsumoto K, Matsubara Y, Yamamoto M, Watanabe A, Nukiwa T, Ichinose M.
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Journal Title
Cancer Sci
Volume: 印刷中
Issue: 6
Pages: 1263-1270
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin.2017
Author(s)
Hidaka, T., Ogawa, E., Kobayashi, E.H., Suzuki, T., Funayama, R., Nagashima, T., Fujimura, T., Aiba, S., Nakayama, K., Okuyama, R., Yamamoto, M.
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Journal Title
Nat. Immunol.
Volume: 18
Issue: 1
Pages: 64-73
DOI
Related Report
Peer Reviewed
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[Journal Article] The SCFβ-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis.2017
Author(s)
Shimizu K, Fukushima H, Ogura K, Lien EC, Nihira NT, Zhang J, North BJ, Guo A, Nagashima K, Nakagawa T, Hoshikawa S, Watahiki A, Okabe K, Yamada A, Toker A, Asara JM, Fukumoto S, Nakayama KI, Nakayama K, Inuzuka H, Wei W.
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Journal Title
Sci Signal. doi: 10.1126/scisignal.aah4117.
Volume: 10
Issue: 460
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Dramatic response after functional hemispherectomy in a patient with epileptic encephalopathy carrying a de novo COL4A1 mutation.2017
Author(s)
Hino-Fukuyo, N., Kikuchi, A., Iwasaki, M., Sato, Y., Kubota, Y., Kobayashi, T., Nakayama, T., Haginoya, K., Arai-Ichinoi, N., Niihori, T., Sato, R., Suzuki, T., Kudo, H., Funayama, R., Nakayama, K., Aoki, Y., Kure, S.
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Journal Title
Brain Dev.
Volume: 39
Issue: 4
Pages: 337-340
DOI
Related Report
Peer Reviewed
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[Journal Article] Lack of genetic variation prevents adaptation at the geographic range margin in a damselfly.2016
Author(s)
Takahashi, Y., Suyama, Y., Matsuki, Y., Funayama, R., Nakayama, K., Kawata, M.
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Journal Title
Mol. Ecol.
Volume: 25
Issue: 18
Pages: 4450-4460
DOI
Related Report
Peer Reviewed
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