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Proteomic mapping of Yb body in live OSCs via APEX tagging

Research Project

Project/Area Number 16K14666
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Molecular biology
Research InstitutionThe University of Tokyo

Principal Investigator

Ishizu Hirotsugu  東京大学, 大学院理学系研究科(理学部), 助教 (40574588)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordspiRNA / Yb body / RNA granule / APEX / プロテオーム / RNA顆粒体 / 定量プロテオーム解析
Outline of Final Research Achievements

Eukaryotic cells contain many kinds of RNP granules; P bodies, stress granules, etc. Several observations suggest that liquid-liquid phase separation is the molecular basis for RNP granule assembly. Weak multivalent interactions between RNA-binding proteins and ssRNAs play a role in RNP granule assembly. Yb bodies are non-membrane-bound organelles consisting of precursor piRNA transcripts and piRNA factor proteins in Drosophila ovarian somatic cells, and are involved in piRNA biogenesis. However, the composition and function of Yb bodies are largely unknown.
Ascorbic acid peroxidese (APEX) has been used to interrogate specific protein networks. In this study, APEX was fused to Yb to capture components of Yb bodies in OSCs. We found that APEX proximity labeling was able to enrich protein components of Yb bodies.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Journal Article] Use of the CRISPR-Cas9 system for genome editing in cultured Drosophila ovarian somatic cells.2017

    • Author(s)
      Ishizu H, Sumiyoshi T, Siomi MC
    • Journal Title

      Methods

      Volume: 126 Pages: 186-192

    • DOI

      10.1016/j.ymeth.2017.05.021

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of Small Noncoding RNAs from the flamenco Locus Is Regulated by the gypsy Retrotransposon of Drosophila melanogaster.2016

    • Author(s)
      Guida V, Cernilogar FM, Filograna A, De Gregorio R, Ishizu H, Siomi MC, Schotta G, Bellenchi GC, Andrenacci D.
    • Journal Title

      Genetics

      Volume: 204 Issue: 2 Pages: 631-644

    • DOI

      10.1534/genetics.116.187922

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Piwi Modulates Chromatin Accessibility by Regulating Multiple Factors Including Histone H1 to Repress Transposons.2016

    • Author(s)
      Iwasaki YW, Murano K, Ishizu H, Shibuya A, Iyoda Y, Siomi MC, Siomi H, Saito K.
    • Journal Title

      Molecular Cell

      Volume: 63 Issue: 3 Pages: 408-419

    • DOI

      10.1016/j.molcel.2016.06.008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Role of the RNA helicase, Armitage, in the primary piRNA biogenesis pathway2017

    • Author(s)
      Ishizu, H., Kinoshita, T., Siomi, MC
    • Organizer
      The 43rd Naito Conference on “NoncodingRNA: Biology, Chemistry, & Diseases”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] piRNA生合成経路によるmRNA分解制御2017

    • Author(s)
      石津大嗣、塩見美喜子
    • Organizer
      第19回日本RNA学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Role of the RNA helicase, Armitage, in the primary piRNA biogenesis pathway2016

    • Author(s)
      Ishizu, H., Kinoshita, T., Siomi, MC.
    • Organizer
      The 2016 joint annual meeting of the RNA Society and the RNA Society of Japan,
    • Place of Presentation
      国立京都国際会館(京都府京都市左京区)
    • Year and Date
      2016-06-28
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 東京大学理学部生物化学科塩見研究室ホームページ

    • URL

      http://www-siomilab.biochem.s.u-tokyo.ac.jp/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 東京大学理学部生物化学科塩見研究室のHP

    • URL

      http://www-siomilab.biochem.s.u-tokyo.ac.jp/index.html

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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