Research Project
Grant-in-Aid for Challenging Exploratory Research
The mismatch repair system not only increases the replication fidelity by correcting replication errors but also improves the recombination fidelity by preventing illegitimate recombination. To study how these reactions are branched and how illegitimate recombination is inhibited, we established an in vitro model system that recapitulates the inhibition of recombination between divergent sequences. We induced single-strand annealing repair of DNA double-strand breaks by adding DNA substrates into nucleoplasmic extracts of Xenopus eggs and found that sequence divergence between the annealing units significantly reduces the annealing reaction. We also found that the mismatch-recognition complex and a RecQ-family helicase prevent annealing of divergent sequences. This system will be useful to study the recombination intermediates and factors that are involved in the inhibition of illegitimate recombination.
All 2017 2016
All Journal Article (4 results) (of which Int'l Joint Research: 3 results, Peer Reviewed: 4 results, Open Access: 4 results, Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results, Invited: 2 results)
Nucleic Acids Research
Volume: 44(22) Issue: 19 Pages: 11222-11235
10.1093/nar/gkx763
Cell Reports
Volume: 21 Issue: 5 Pages: 1375-1385
10.1016/j.celrep.2017.10.032
eLIFE
Volume: 12;5
10.7554/elife.15155
Volume: 16 Issue: 3 Pages: 684-695
10.1016/j.celrep.2016.06.030
