A unified understanding of replication error correction and antirecombination mediated by the mismatch repair system
Project/Area Number |
16K14671
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Molecular biology
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Research Institution | Kyushu University |
Principal Investigator |
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Research Collaborator |
Oda Satomi
Kawasoe Yoshitaka
Sakazume Aya
Terui Riki
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Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | ミスマッチ修復 / 相同組換え / ツメガエル卵抽出液 / 無細胞系 / DNA二重鎖切断損傷 / 相同性依存的修復 / 突然変異 / ゲノム安定性 / 相同組み換え / DNA修復 / DNA組換え |
Outline of Final Research Achievements |
The mismatch repair system not only increases the replication fidelity by correcting replication errors but also improves the recombination fidelity by preventing illegitimate recombination. To study how these reactions are branched and how illegitimate recombination is inhibited, we established an in vitro model system that recapitulates the inhibition of recombination between divergent sequences. We induced single-strand annealing repair of DNA double-strand breaks by adding DNA substrates into nucleoplasmic extracts of Xenopus eggs and found that sequence divergence between the annealing units significantly reduces the annealing reaction. We also found that the mismatch-recognition complex and a RecQ-family helicase prevent annealing of divergent sequences. This system will be useful to study the recombination intermediates and factors that are involved in the inhibition of illegitimate recombination.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Regulation of mitotic recombination between DNA repeats in centromeres.2017
Author(s)
Zafar,F., Okita,AK. Onaka,AT., Su,J., Katahira,Y., Nakayama,J. Takahashi,TS., Masukata, H., Nakagawa, T.
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Journal Title
Nucleic Acids Research
Volume: 44(22)
Issue: 19
Pages: 11222-11235
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Sensing and Processing of DNA Interstrand Crosslinks by the Mismatch Repair Pathway.2017
Author(s)
Kato N, Kawasoe Y, Williams H, Coates E, Roy U, Shi Y, Beese LS, Scharer OD, Yan H, Gottesman ME, Takahashi TS, Gautier J.
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Journal Title
Cell Reports
Volume: 21
Issue: 5
Pages: 1375-1385
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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