| Project/Area Number |
16K14673
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
KITAGAWA DAIJU 国立遺伝学研究所, 分子遺伝研究系, 教授 (80605725)
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| Co-Investigator(Renkei-kenkyūsha) |
Shiratsuchi Gen 国立遺伝学研究所, 分子遺伝研究系, 博士研究員 (80625533)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | lncRNA / 細胞分裂 / 次世代シークエンス / 紡錘体 / 中心体 / 遺伝子 / 核酸 / 癌 / 生体分子 |
|---|
| Outline of Final Research Achievements |
In eukaryotic cells, the formation of bipolar spindles is crucial for accurate segregation of chromosomes in mitosis, and therefore it must be strictly regulated for the maintenance of genome integrity. Long non-coding RNAs (lncRNAs) have been recently revealed to function in a wide range of biological phenomena including cell proliferation and tumorigenesis. Interestingly, the existence of spindle-bound RNAs and their importance in mitosis have been previously predicted in human. However, the lncRNAs that function in specific mitotic processes remain to be identified to date. In this study, we identified two uncharacterized human lncRNAs as novel regulators of mitotic spindle formation and centriole formation, using a combination of next-generation sequencing, bioinformatics and biochemistry.
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