| Project/Area Number |
16K14679
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIWARA Takahiro 京都大学, 物質-細胞統合システム拠点, 准教授 (80423060)
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | ダイアモンドNVC / ダイヤモンド / NVC / １分子計測 / 蛍光イメージング / 磁気共鳴 |
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| Outline of Final Research Achievements |
In this study, an application of ODMR(Optically Detected Magnetic Resonance) of NVC of nanodiamonds to analyzing protein structures was examined. Nanodiamonds artificially enriched with NVCs were covalently conjugated to membranous proteins on the plasma membrane. The movement of the proteins were successfully tracked on the membrane with the fluorescence of NVC with a TIRF microscope. Then detection of ODMR was attempted. However, ODMR signals were not obtained successfully, which was mainly due to low signal to noise ratio of the fluorescence signals. Through this study, requirements for the system to achieve the ODMR detection has been revealed, which would be addressed in the future research projects.
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