| Project/Area Number |
16K14681
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Yokohama National University |
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Principal Investigator |
Kojima Chojiro 横浜国立大学, 大学院工学研究院, 教授 (50333563)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Naohiro 大阪大学, 蛋白質研究所, 特任准教授 (80272160)
SUGIKI Toshihiko 大阪大学, 蛋白質研究所, 特任助教 (70635698)
FURUITA Kyoko 大阪大学, 蛋白質研究所, 特任助教 (30727665)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | NMR / 蛋白質 / 立体構造 / 自動化 / 自動構造決定 |
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| Outline of Final Research Achievements |
Molecular replacement method is a key technique to accelerate the protein structure determination by X-ray crystallography. For NMR, no such method is available, and thus, it still takes time to determine protein structure. In this research, we have developed an NMR method to accelerate the protein structure determination. Our method is based on software MagRO and FLYA/CYANA developed by Prof Kobayashi and Prof Guentert, and validated by some NMR parameters, including RDC values. Finally, we have succeeded to determine protein structure fully automatically within half day.
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