NMR Molecular Replacement Method
Project/Area Number |
16K14681
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
|
Research Institution | Yokohama National University |
Principal Investigator |
Kojima Chojiro 横浜国立大学, 大学院工学研究院, 教授 (50333563)
|
Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Naohiro 大阪大学, 蛋白質研究所, 特任准教授 (80272160)
SUGIKI Toshihiko 大阪大学, 蛋白質研究所, 特任助教 (70635698)
FURUITA Kyoko 大阪大学, 蛋白質研究所, 特任助教 (30727665)
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | NMR / 蛋白質 / 立体構造 / 自動化 / 自動構造決定 |
Outline of Final Research Achievements |
Molecular replacement method is a key technique to accelerate the protein structure determination by X-ray crystallography. For NMR, no such method is available, and thus, it still takes time to determine protein structure. In this research, we have developed an NMR method to accelerate the protein structure determination. Our method is based on software MagRO and FLYA/CYANA developed by Prof Kobayashi and Prof Guentert, and validated by some NMR parameters, including RDC values. Finally, we have succeeded to determine protein structure fully automatically within half day.
|
Report
(3 results)
Research Products
(21 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Publication of nuclear magnetic resonance experimental data with semantic web technology and the application thereof to biomedical research of proteins2016
Author(s)
Masashi Yokochi, Naohiro Kobayashi, Eldon L. Ulrich, Akira R. Kinjo, Takeshi Iwata, Yannis E. Ioannidis, Miron Livny, John L. Markley, Haruki Nakamura, Chojiro Kojima, and Toshimichi Fujiwara
-
Journal Title
J. Biomed. Semant.
Volume: 7
Pages: 16-16
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
[Journal Article] The Structure Determination of AgI-mediated Cytosine-Cytosine Base Pair within DNA Duplex in Solution with 1H/15N/109Ag NMR Spectroscopy2016
Author(s)
Takenori Dairaku, Kyoko Furuita, Hajime Sato, Jakub Sebera, Katsuyuki Nakashima, Jiro Kondo, Daichi Yamanaka, Yoshinori Kondo, Itaru Okamoto, Akira Ono, Vladimir Sychrovsky, Chojiro Kojima, and Yoshiyuki Tanaka
-
Journal Title
Chemistry
Volume: 22
Issue: 37
Pages: 13028-13031
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
-
[Journal Article] Noncovalent forces tune the electron transfer complex between ferredoxin and sulfite reductase to optimize enzymatic activity2016
Author(s)
Ju Yaen Kim, Misaki Kinoshita, Satoshi Kume, Guy T. Hanke, Toshihiko Sugiki, John E. Ladbury, Chojiro Kojima, Takahisa Ikegami, Genji Kurisu, Yuji Goto, Toshiharu Hase, and Young-Ho Lee
-
Journal Title
Biochem. J.
Volume: 473
Pages: 3837-3854
Related Report
Peer Reviewed / Int'l Joint Research
-