| Project/Area Number |
16K14694
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Masuda Shinji 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (30373369)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 崇之 東京工業大学, 生命理工学院, 准教授 (60612760)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 緊縮応答 / 栄養応答 / ppGpp / ショウジョウバエ / 栄養欠乏応答 |
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| Outline of Final Research Achievements |
We previously established the quantification method for detecting ppGpp in plant tissues by use of LC-MS/MS technology. Here, we re-established the method to detect ppGpp in other organisms. As a results, we could detect some ppGpp not in plant tissues, but also algae and other organisms. We also try to express a bacterial ppGpp synthase in animal cells. We succeeded to obtain recombinant organisms that express the ppGpp synthase in different tissues. We found that over-production of ppGpp in the organisms results in significant behavioral responses in the organisms. This suggest that ppGpp-dependent stringent response could, at least, be operated in animals.
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