The stringent response in animals
Project/Area Number |
16K14694
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
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Research Institution | Tokyo Institute of Technology |
Principal Investigator |
Masuda Shinji 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (30373369)
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Co-Investigator(Kenkyū-buntansha) |
鈴木 崇之 東京工業大学, 生命理工学院, 准教授 (60612760)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 緊縮応答 / 栄養応答 / ppGpp / ショウジョウバエ / 栄養欠乏応答 |
Outline of Final Research Achievements |
We previously established the quantification method for detecting ppGpp in plant tissues by use of LC-MS/MS technology. Here, we re-established the method to detect ppGpp in other organisms. As a results, we could detect some ppGpp not in plant tissues, but also algae and other organisms. We also try to express a bacterial ppGpp synthase in animal cells. We succeeded to obtain recombinant organisms that express the ppGpp synthase in different tissues. We found that over-production of ppGpp in the organisms results in significant behavioral responses in the organisms. This suggest that ppGpp-dependent stringent response could, at least, be operated in animals.
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Report
(2 results)
Research Products
(13 results)
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[Journal Article] Sulfide-responsive transcriptional repressor SqrR functions as a master regulator of sulfide-dependent photosynthesis2017
Author(s)
Shimizu, T., Shen, J., Fang, M., Zhang, Y., Hori, K., Trinidad, J.C., Bauer, C.E., Giedroc, D.P. and Masuda S.
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 114
Issue: 9
Pages: 2355-2360
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] A novel chloroplast membrane protein, conserved in oxygenic phototrophs, regulates ion homeostasis and non-photochemical quenching induction2016
Author(s)
Sato, R., Kono, M., Ohta, H., Takaichi, S. and Masuda, S.
Organizer
The 17th International Congress on Photosynthesis Research
Place of Presentation
Maastricht, Netherlands
Year and Date
2016-08-07
Related Report
Int'l Joint Research
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