Quality control system for the newly synthesized tail-anchored proteins.
| Project/Area Number |
16K14699
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | BAG6 / ユビキチン / プロテアソーム / テイルアンカー蛋白質 / タンパク質品質管理 / タンパク質分解 / タンパク質凝集 / シグナル配列 / テイルアンカータンパク質 / BAG6複合体 / UBQLN4 / BUILDドメイン / 構造不良膜タンパク質 / TAタンパク質 |
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| Outline of Final Research Achievements |
A portion of newly synthesized tail-anchored proteins tend to fail their correct assembly into the lumen of endoplasmic reticulum, thus resulting in the production of the defective species. Although the efficient degradation of these aggregation-prone polypeptides is crucial, the molecular mechanism of their elimination pathway has not been adequately characterized. In this study, we focused on one such cryptic portion of the defective transmembrane domain protein, and show that a part of these is produced as a labile species that is immediately targeted to the degradation pathway. We found that proteasomes are indispensable for elimination of mislocalized tail-anchored species. These observations suggest that protein degradation machinery acts as a critical factor for degradation of mislocalized tail-anchored proteins.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] Novel phosphorelay-dependent control of ZFP36L1 protein during the cell cycle.2018
Author(s)
Kondo, M., Noguchi, A., Matsuura, Y., Shimada, M., Yokota, N., Kawahara, H.
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Journal Title
Biochem. Biophys. Res. Comm.
Volume: in press
Issue: 2
Pages: 387-393
DOI
Related Report
Peer Reviewed
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[Journal Article] Elimination of a signal-sequence uncleaved form of defective HLA protein through BAG6.2017
Author(s)
Yamamoto, K., Hayashishita, M., Minami, S., Suzuki, K., Hagiwara, T., Noguchi, A., and Kawahara, H.
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Journal Title
Sci. Rep.
Volume: 7
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A conserved island of BAG6/Scythe is related to ubiquitin domains and participates in short hydrophobicity recognition.2016
Author(s)
Tanaka, H., Takahashi, T., Xie, Y., Minami, R., Yanagi, Y., Hayashishita, M., Suzuki, R., Yokota, N., Shimada, M., Mizushima, T., Kuwabara, N., Kato, R., Kawahara, H.
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Journal Title
FEBS J.
Volume: 283
Issue: 4
Pages: 662-677
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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