Measure the temperature of specific biomolecules in cells
| Project/Area Number |
16K14705
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Funatsu Takashi 東京大学, 大学院薬学系研究科(薬学部), 教授 (00190124)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
OKABE Kohki 東京大学, 大学院薬学系研究科, 助教 (20455398)
|
|---|
| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | ナノバイオ / 細胞・組織 / 生体分子 / 分析科学 / 生物物理 |
|---|
| Outline of Final Research Achievements |
The Raman spectrum of O-H stretching vibration of water molecule is broad, and it is well known that two peaks are observed and that the isosbestic point is 3419 cm-1. As the temperature gets higher, the peak on the low Raman shift side becomes smaller and the peak on the high Raman shift side becomes larger, so we could measure the temperature using this property. We also measured Raman spectra of low frequency oscillations such as C-C-N bending vibration (458 cm-1) and Te-Te stretching vibration (150 cm-1) of diphenyl ditelluride. We could measure the temperature in the microscopic region (in the order of micrometers) from the ratio of Stokes and anti-Stokes light intensities. It is expected that Raman temperature measurement within a cell will be possible by synthesizing a probe combining a compound exhibiting low frequency Raman oscillation and a compound accumulating in specific organelle.
|
Report
(3 results)
Research Products
(24 results)
-
[Journal Article] A micromachined liquid chromatography chip with a pillar array mixer for post-column derivatization in the analysis of neurotransmitters2017
Author(s)
Muneki Isokawa, Kanki Nakanishi, Takahiro Kanamori, Huiqi Zhuang, Hayate Yamazaki, Takuo Sugaya, Dong Hyun Yoon, Tetsushi Sekiguchi, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda
-
Journal Title
The proceedings of μTAS 2017
Volume: 21
Pages: 1318-1319
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
