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Measure the temperature of specific biomolecules in cells

Research Project

Project/Area Number 16K14705
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionThe University of Tokyo

Principal Investigator

Funatsu Takashi  東京大学, 大学院薬学系研究科(薬学部), 教授 (00190124)

Co-Investigator(Renkei-kenkyūsha) OKABE Kohki  東京大学, 大学院薬学系研究科, 助教 (20455398)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsナノバイオ / 細胞・組織 / 生体分子 / 分析科学 / 生物物理
Outline of Final Research Achievements

The Raman spectrum of O-H stretching vibration of water molecule is broad, and it is well known that two peaks are observed and that the isosbestic point is 3419 cm-1. As the temperature gets higher, the peak on the low Raman shift side becomes smaller and the peak on the high Raman shift side becomes larger, so we could measure the temperature using this property. We also measured Raman spectra of low frequency oscillations such as C-C-N bending vibration (458 cm-1) and Te-Te stretching vibration (150 cm-1) of diphenyl ditelluride. We could measure the temperature in the microscopic region (in the order of micrometers) from the ratio of Stokes and anti-Stokes light intensities. It is expected that Raman temperature measurement within a cell will be possible by synthesizing a probe combining a compound exhibiting low frequency Raman oscillation and a compound accumulating in specific organelle.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2017 2016 Other

All Journal Article (3 results) Presentation (20 results) (of which Int'l Joint Research: 7 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] A micromachined liquid chromatography chip with a pillar array mixer for post-column derivatization in the analysis of neurotransmitters2017

    • Author(s)
      Muneki Isokawa, Kanki Nakanishi, Takahiro Kanamori, Huiqi Zhuang, Hayate Yamazaki, Takuo Sugaya, Dong Hyun Yoon, Tetsushi Sekiguchi, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda
    • Journal Title

      The proceedings of μTAS 2017

      Volume: 21 Pages: 1318-1319

    • Related Report
      2017 Annual Research Report
  • [Journal Article] マイクロ・ナノデバイスを用いた生体分子の機能解析と有用遺伝子の探索・回収2017

    • Author(s)
      藤井聡一郎、中村和貴、飯塚怜、船津高志
    • Journal Title

      Molecular Electronics and Bioelectronics

      Volume: 28 Pages: 165-168

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 全反射蛍光顕微鏡による細胞膜受容体MPL二量体の1分子イメージング2017

    • Author(s)
      坂本明彦、船津高志
    • Journal Title

      生体の科学

      Volume: 68 Pages: 386-387

    • Related Report
      2017 Annual Research Report
  • [Presentation] Single-molecule observation of actin polymerizationusing linear zero-mode waveguides2017

    • Author(s)
      Soichiro Fujii, Ryo Iizuka, Masamichi Yamamoto, Makoto Tsunoda, Takashi Tanii, Takashi Funatsu
    • Organizer
      Joint Symposium on Bioimaging between Singapore and Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a method of heating a single cell using gold nanoparticles2017

    • Author(s)
      Takaaki Honda, Kohki Okabe, Takashi Funatsu
    • Organizer
      Joint Symposium on Bioimaging between Singapore and Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of residues in SecM important for stabilizing translation arrest2017

    • Author(s)
      Takashi Funatsu, Mikihisa Muta, Ryo Iizuka
    • Organizer
      International Symposium on Protein Quality Control
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Intracellular local temperature as a novel variable in cell biology2017

    • Author(s)
      Kohki Okabe, Masahiro Takinoue, Kazuhito V. Tabata, Takashi Funatsu
    • Organizer
      The joint 19th International Union of Pure and Applied Biophysics (IUPAB) and 11th European Biophysical Societies' Association (EBSA) Congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Intracellular local termogemnesis initiates stress granule formation2017

    • Author(s)
      Shi Beini, Kouki Okabe, Takashi Funatsu
    • Organizer
      The joint 19th International Union of Pure and Applied Biophysics (IUPAB) and 11th European Biophysical Societies' Association (EBSA) Congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロ・ナノデバイスを用いた生体分子の機能解析と有用遺伝子の探索・回収2017

    • Author(s)
      船津高志
    • Organizer
      新分野開拓研究会「有機・バイオエレクトロニクスにおける先端計測技術の進展」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 蛍光分子の明滅計測による細胞内微小環境のマルチモーダルイメージング2017

    • Author(s)
      高田涼子、岡部弘基、菅原皓、船津高志
    • Organizer
      第30回バイオメディカル分析科学シンポジウム(BMAS2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Single-molecule analysis of actin polymerization using linear zero-mode waveguides2017

    • Author(s)
      Soichiro Fujii, Ryo Iizuka, Masamichi Yamamoto, Makoto Tsunoda, Takashi Tanii, Takashi Funatsu
    • Organizer
      第26回日本バイオイメージング学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Intercellular temperature measurement during RNA granule formation2017

    • Author(s)
      Beini Shi, Kohki kabe, Takashi Funatsu
    • Organizer
      第26回日本バイオイメージング学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Investigating the contribution of microtubules on intracellular temperature variation2017

    • Author(s)
      Takashi Yanagi, Kohki Okabe, Takashi Funatsu
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Intracellular temperature measurement during RNA granule formation2017

    • Author(s)
      Beini Shi, Kohki Okabe, Takashi Funatsu
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of a method of local heating a single cell using gold nanoparticles2017

    • Author(s)
      Takaaki Honda, Kohki Okabe, Takashi Funatsu
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Single-molecule analysis of actin polymerization mechanism using linear zero-mode waveguides2017

    • Author(s)
      Soichiro Fujii, Ryo Iizuka, Masamichi Yamamoto, Makoto Tsunoda, Takashi Tanii, Takashi Funatsu
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Investigating contribution of transcription to temperature in nucleus2017

    • Author(s)
      Shunsuke Takeda, Kohki Okabe, Takashi Funatsu
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Investigating molecular mechanism of intracellular temperature dependent cell functions2017

    • Author(s)
      Yu Bi, Kohki Okabe, Takashi Funatsu
    • Organizer
      第55回日本生物物理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 1蛍光分子の明滅計測による細胞内微小環境のマルチモーダルイメージング2017

    • Author(s)
      高田涼子、岡部弘基、菅原皓、船津高志
    • Organizer
      2017年生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞内微小空間における温度の計測と操作から解明する温度シグナリング2017

    • Author(s)
      岡部弘基、時ベイニ、船津高志
    • Organizer
      2017年生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Development of fluorescent thermometers for mapping intercellular temperature2016

    • Author(s)
      Takashi Funatsu, Kohki Okabe
    • Organizer
      The 12th IUPAC International Conference on Novel Materials and their Synthesis (NMS-XII)
    • Place of Presentation
      Huna Agricultural University, Changsha, China
    • Year and Date
      2016-10-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 3つの生命現象を見えるようにする挑戦:細胞温度、アクチン重合、ストレス顆粒2016

    • Author(s)
      船津高志
    • Organizer
      レーザ顕微鏡研究会 第42回講演会(SLM-42)
    • Place of Presentation
      理化学研究所和光キャンパス、和光市、埼玉県
    • Year and Date
      2016-07-07
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Single-molecule fluorescence imaging by zero-mode waveguides2016

    • Author(s)
      Takashi Funatsu
    • Organizer
      Energy, Material & Nanotechnology Meeting On Light Matter Interactions
    • Place of Presentation
      Peninsula Excelsior Hotel, Singapore
    • Year and Date
      2016-05-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 生体分析化学教室 研究業績

    • URL

      http://www.f.u-tokyo.ac.jp/~funatsu/jp/publication.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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