Quantitative analysis of ATP dynamics in photosynthetic organisms by a novel bioluminescence ATP biosensor
| Project/Area Number |
16K14709
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
Imamura Hiromi 京都大学, 生命科学研究科, 准教授 (20422545)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生物発光 / BRET / イメージング / 光合成 / ATP / ライブイメージング / エネルギー代謝 |
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| Outline of Final Research Achievements |
We developed a novel genetically encoded BRET-based ATP biosensor, BTeam. It reacts specifically to ATP, and reports ATP concentrations in a range of 0.5 - 6.0 mM as BRET efficiencies. By measuring BRET efficiencies of BTeam-expressing cultured cells, we were able to quantitatively estimate ATP concentrations inside living cells. We also demonstrated that BTeam can report ATP concentrations inside a living organism using a zebrafish expressing BTeam. Currently, we are constructing a plant expressing BTeam in order to monitor ATP dynamics in plants during photosynthesis.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure.2017
Author(s)
Suzuki, M., Sato, M., Koyama, H., Hara, Y., Hayashi, K., Yasue, N., Imamura, H., Fujimori, T., Nagai, T., Campbell, RE., Ueno, N.
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Journal Title
Development.
Volume: 144
Issue: 7
Pages: 1307-1316
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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