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Piezoelectric effect in proteins: a novel mechanism of allostery

Research Project

Project/Area Number 16K14715
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionWaseda University

Principal Investigator

Takano Mitsunori  早稲田大学, 理工学術院, 教授 (40313168)

Research Collaborator Sato Takato  
Ohnuki Jun  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsアロステリー / 分子機械 / 圧電応答 / 誘電応答 / アロステリック機構 / タンパク質 / 圧電効果
Outline of Final Research Achievements

The vital activities of living organisms are ultimately based on the functions of protein molecules and the highly-organized intermolecular interaction network among the proteins and other biomolecules. The motility, for example, is based on the association and dissociation dynamics of actin and myosin molecules that is regulated by ATP (energy-supplying biomolecule). In this study, by using high-performance computers, we elucidated the input-output characteristics (particularly piezoelectric and dielectric responses) of the molecular device of protein to advance the physical understanding of how a single protein molecule works and how the intermolecular interactions are regulated.

Academic Significance and Societal Importance of the Research Achievements

もしミオシンの正常に機能しなくなったり、あるいはATPによるミオシンとアクチンの結合と解離の制御がうまくいかなくなることあれば、私たちの運動機能(たとえば心臓の収縮弛緩運動)は大きなダメージを受ける。タンパク質1分子がどのように機能するか、分子間相互作用がどのように制御されるかの物理基盤が明らかになればタンパク質分子物性の基礎科学として学術的意義が大きいのみならず、それらが正常に機能しなくなったときの根本的な原因究明(原因療法)につながる可能性がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (29 results)

All 2019 2018 2017 2016

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 4 results) Presentation (18 results) (of which Int'l Joint Research: 8 results,  Invited: 3 results) Book (2 results)

  • [Journal Article] Hydrophobic Surface Enhances Electrostatic Interaction in Water2018

    • Author(s)
      Sato Takato、Sasaki Tohru、Ohnuki Jun、Umezawa Koji、Takano Mitsunori
    • Journal Title

      Physical Review Letters

      Volume: 121 Issue: 20 Pages: 206002-206002

    • DOI

      10.1103/physrevlett.121.206002

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Orchestrated Electrostatic Interactions Among Myosin, Actin, ATP, and Water2018

    • Author(s)
      Takano Mitsunori
    • Journal Title

      The Role of Water in ATP Hydrolysis Energy Transduction by Protein Machinery

      Volume: - Pages: 113-122

    • DOI

      10.1007/978-981-10-8459-1_8

    • ISBN
      9789811084584, 9789811084591
    • Related Report
      2018 Annual Research Report
  • [Journal Article] Structural and dynamical characteristics of tropomyosin epitopes as the major allergens in shrimp2018

    • Author(s)
      Ozawa Hideo、Umezawa Koji、Takano Mitsunori、Ishizaki Shoichiro、Watabe Shugo、Ochiai Yoshihiro
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 498 Issue: 1 Pages: 119-124

    • DOI

      10.1016/j.bbrc.2018.02.172

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Long-range coupling between ATP-binding and lever-arm regions in myosin via dielectric allostery2017

    • Author(s)
      Sato Takato、Ohnuki Jun、Takano Mitsunori
    • Journal Title

      The Journal of Chemical Physics

      Volume: 147 Issue: 21 Pages: 215101-215101

    • DOI

      10.1063/1.5004809

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Electrostatic balance between global repulsion and local attraction in reentrant polymerization of actin2017

    • Author(s)
      Ohnuki Jun、Yodogawa Akira、Takano Mitsunori
    • Journal Title

      Cytoskeleton

      Volume: 74 Issue: 12 Pages: 504-511

    • DOI

      10.1002/cm.21391

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Over-destabilization of protein-protein interaction in Generalized Born model and utility of energy density integration cutoff2017

    • Author(s)
      Y. Mizuhara, D. Parkin, K. Umezawa, J. Ohnuki, M. Takano
    • Journal Title

      J. Phys. Chem. B

      Volume: 121 Issue: 18 Pages: 4669-4677

    • DOI

      10.1021/acs.jpcb.7b01438

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Piezoelectric allostery of protein2016

    • Author(s)
      J. Ohnuki, T. Sato, M. Takano
    • Journal Title

      Phys. Rev. E

      Volume: 94 Issue: 1 Pages: 012406-012406

    • DOI

      10.1103/physreve.94.012406

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dielectric allostery of protein: Response of myosin to ATP binding2016

    • Author(s)
      T. Sato, J. Ohnuki, M. Takano
    • Journal Title

      J. Phys. Chem. B

      Volume: 120 Issue: 51 Pages: 13047-13055

    • DOI

      10.1021/acs.jpcb.6b10003

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Intrinsic disorder accelerates dissociation rather than association2016

    • Author(s)
      K. Umezawa, J. Ohnuki, J. Higo, and M. Takano
    • Journal Title

      Proteins: Structure, Function and Bioinformatics

      Volume: - Issue: 8 Pages: 1489-1501

    • DOI

      10.1002/prot.25057

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Depolymerization mechanism of actin due to dielectric allostery2019

    • Author(s)
      Jun Ohnuki, Mitsunori Takano
    • Organizer
      Biophysical Society 63rd Annual Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 誘電アロステリ―によるアクチンを基盤とした生体運動の物理機構2019

    • Author(s)
      大貫隼、高野光則
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] Coulombic interaction network and novel allostery in molecular machines2018

    • Author(s)
      Mitsunori Takano
    • Organizer
      56th Annual Meeting of Biophysical Society of Japan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Piezoelectric and dielectric allostery of an actin filament and its effect on binding preference of cofilin2018

    • Author(s)
      Jun Ohnuki, Akira Yodogawa, Takato Sato, Mitsunori Takano
    • Organizer
      56th Annual Meeting of Biophysical Society of Japan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Coupling of the Redox and Structural States in Cytochrome P450 Reductase Studied by Molecular Dynamics Simulation2018

    • Author(s)
      Mikuru Iijima, Jun Ohnuki, Takato Sato, Masakazu Sugishima, Mitsunori Takano
    • Organizer
      The Protein Society 32nd Annual Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 分子機械の圧電アロステリ―:アクチンフィラメントのメカノセンシングの物理機構2018

    • Author(s)
      大貫隼、高野光則
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2017 Research-status Report
  • [Presentation] 分子機械の誘電アロステリ―:ATP結合によるリバースジャイレースの超らせん導入の物理機構2018

    • Author(s)
      森竹亮太、佐藤昂人、高野光則
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2017 Research-status Report
  • [Presentation] アクチンの荷電状態とサブユニット間静電相互作用のpH依存性2018

    • Author(s)
      山下昴、大貫隼、高野光則
    • Organizer
      第7回日本生物物理学会関東支部会
    • Related Report
      2017 Research-status Report
  • [Presentation] アクチンフィラメントの圧電アロステリ―2017

    • Author(s)
      大貫隼、高野光則
    • Organizer
      第7回分子モーター討論会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Mechano-electrical communications in actin filament2017

    • Author(s)
      大貫隼、高野光則
    • Organizer
      日本生物物理学会第55回年会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Tension-induced electrostatic change in actin: piezoelectricity of an actin filament2017

    • Author(s)
      大貫隼、淀川良、佐藤昂人、上田太郎、高野光則
    • Organizer
      日本生物物理学会第55回年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Counter-ion-induced electrostatic change in actin: the balance between global repulsion and local attraction in reentrant polymerization2017

    • Author(s)
      淀川良、大貫隼、高野光則
    • Organizer
      日本生物物理学会第55回年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 誘電アロステリ―による分子モーターの力発生機構2017

    • Author(s)
      佐藤昂人、大貫隼、高野光則
    • Organizer
      第31回分子シミュレーション討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] シトクロムP450還元酵素の誘電応答が制御する大規模構造変化と電子伝達2017

    • Author(s)
      飯島美来、佐藤昂人、森竹亮太、高野光則
    • Organizer
      第31回分子シミュレーション討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] Piezoelectric Allostery of an Actin Filament2016

    • Author(s)
      J. Ohnuki, T. Sato, H. Okamura, T.Q.P. Uyeda, M. Takano
    • Organizer
      IGER International Symposium on "Now in actin"
    • Place of Presentation
      名古屋
    • Year and Date
      2016-12-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Piezoelectric Allostery of Protein2016

    • Author(s)
      J. Ohnuki, T. Sato, M. Takano
    • Organizer
      Protein Electrostatics Berlin 2016
    • Place of Presentation
      Berlin
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Critical Role of Cutoff Parameter to Calculate Effective Born Radii in Simulating Protein-Protein Interaction2016

    • Author(s)
      Y. Mizuhara, D. Parkin, M. Takano
    • Organizer
      Protein Electrostatics Berlin 2016
    • Place of Presentation
      Berlin
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Piezoelectric property of an actin filament III2016

    • Author(s)
      大貫隼、佐藤昂人、岡村英世、上田太郎、高野光則
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] The Role of Water in ATP Hydrolysis Energy Transduction by Protein Machinery (M. Suzuki Ed.)2018

    • Author(s)
      Mitsunori Takano
    • Total Pages
      10
    • Publisher
      Springer
    • Related Report
      2017 Research-status Report
  • [Book] 分子マシンの科学2017

    • Author(s)
      日本化学会編
    • Total Pages
      200
    • Publisher
      化学同人
    • ISBN
      9784759813869
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2021-01-27  

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