| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
We investigated the phosphorylation that occurs on Cys residue in oncogenic protein PRL. When magnesium ion was depleted from the culture medium, PRL was rapidly dephosphorylated and its protein levels were increased. PRL knockdown under this magnesium ion-depleted condition resulted in promotion of cell death, indicating the biological importance of this response. Moreover, the investigation of the phosphorylation mechanism revealed the possible role of phosphate-containing molecules, such as ATP, as phosphate donor.
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