| Project/Area Number |
16K14733
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | mRNA / 光遺伝学 / 転写 / 発生生物学 / ゼブラフィッシュ / 翻訳 / 光応答性核酸 |
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| Outline of Final Research Achievements |
Living processes are managed through the precise control of “when, where, and how long” proteins are expressed. The photo-control of protein expression enables the spatiotemporal induction of biological events in living cells or organisms. In transcriptional control, several hours elapse between light illumination and the beginning of protein expression, and protein continues to be synthesized for more than 10 hours after the light is turned off due to residual mRNA. Now, I developed a photoresponsive cap that can control the translation of mRNA in a reversible manner via its cis-trans photoisomerization through illumination with 370 nm and 430 nm light. An application of this approach was demonstrated by photo-inducing the development of double-headed zebrafish by controlling the expression of squint protein
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