| Project/Area Number |
16K14734
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
KOBAYASHI Kumiko
ISOMURA Akihiro , さきがけ研究員
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 分節時計 / 遺伝子発現振動 / 同期化 / ライブイメージング / 光遺伝学 / Notchシグナル / Hes7 / Delta-like1 / 発現振動 / 位相 / 未分節中胚葉 |
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| Outline of Final Research Achievements |
We generated transgenic mice carrying a new Hes7 reporter, and succeeded in live imaging of oscillatory expression at the single-cell resolution in the segmentation clock. We also generated cells in which the expression of the Notch ligand Delta-like1 (Dll1) is induced by an optogenetic method. By using these tools, it is now possible to perform live-imaging analysis of the effect on cells by optogenetic perturbation of oscillation to analyze the mechanism of synchronization of oscillatory gene expression between cells in the segmentation clock.
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