Generation of reporter mice by electroporating genome editing system into mouse zygotes

Research Project

Project/Area Number	16K14738
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Developmental biology
Research Institution	The University of Tokushima
Principal Investigator	TAKEMOTO Tatsuya 徳島大学, 先端酵素学研究所(オープンイノベ), 教授 (30443899)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	ゲノム編集 / エレクトロポレーション / レポーターマウス / 発生工学 / イメージング
Outline of Final Research Achievements	This research study established the electroporation method generating reporter mice with genome editing technology. We introduced the components of CRIPSR/Cas9 system including Cas9 protein, crRNA and tracrRNA, together with donor DNA encoding the fluorescent protein such as EGFP or mCherry into mouse zygotes by electroporation. The resulting mouse embryos showed fluorescence where the endogenous target genes are expressed.