Identification of the re-programming function domain existing in ribosome
| Project/Area Number |
16K14741
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
OHTA KUNIMASA 熊本大学, 大学院生命科学研究部(医), 准教授 (90244128)
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| Research Collaborator |
ITO NAOFUMI 熊本大学, 大学院生命科学研究部, 研究員 (60732716)
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | リボソーム / 幹細胞 / リプログラミング |
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| Outline of Final Research Achievements |
I homogenized the lactic acid bacteria (LAB) ,separated them to each fraction by chromatography, and performed the cell cluster formation assay. I found that a single fraction has cell cluster formation activity and it contained some proteins derived from the ribosome. Then, I purified the ribosome from LAB by the ultra centrifugation, and performed the same cell cluster formation assay. Interestingly, it showed the cell clusters formation. From these results, I confirmed that the reprogramming material from LAB is ribosome (Ito et al., revised).
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Report
(2 results)
Research Products
(10 results)
