Identification of the re-programming function domain existing in ribosome

• Project/Area Number: 16K14741
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: OHTA KUNIMASA 熊本大学, 大学院生命科学研究部(医), 准教授 (90244128)
• Research Collaborator: ITO NAOFUMI 熊本大学, 大学院生命科学研究部, 研究員 (60732716)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: リボソーム / 幹細胞 / リプログラミング

Outline of Final Research Achievements

I homogenized the lactic acid bacteria (LAB) ,separated them to each fraction by chromatography, and performed the cell cluster formation assay. I found that a single fraction has cell cluster formation activity and it contained some proteins derived from the ribosome. Then, I purified the ribosome from LAB by the ultra centrifugation, and performed the same cell cluster formation assay. Interestingly, it showed the cell clusters formation. From these results, I confirmed that the reprogramming material from LAB is ribosome (Ito et al., revised).

Research Products

• [Presentation] Ribosome incorporation into somatic cells promotes reprogramming towards multipotency without activating cell proliferation (2017)
  • Author(s): 太田訓正
  • Organizer: Joint Meeting of the German and Japanese Societiesof Developmental Biologists
  • Place of Presentation: ドイツ連邦共和国(キール)、キール大学
  • Year and Date: 2017-03-15
  • Int'l Joint Research / Invited
• [Presentation] リボゾームによる細胞の多能性獲得機能 (2017)
  • Author(s): 太田訓正
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 宮城県仙台市、仙台国際センター
  • Year and Date: 2017-03-07
• [Presentation] Bacterial ribosome incorporation into somatic cells promotes reprogramming towards multipotency without activating cell proliferation (2016)
  • Author(s): 伊藤尚文
  • Organizer: 第６回オルソオレガノジェネシス検討会
  • Place of Presentation: 沖縄県恩納村、リザンシーパークホテル、シーサイドハウス(沖縄科学技術大学院大学OIST)
  • Year and Date: 2016-12-06
  • Int'l Joint Research
• [Presentation] Ribosome Incorporation into Somatic Cells Promotes Reprogramming towards Multipotency without Activating Cell Proliferation (2016)
  • Author(s): 伊藤尚文
  • Organizer: 第39回日本分子生物学
  • Place of Presentation: 神奈川県横浜市、パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] Ribosome Incorporation into Somatic Cells Promotes Reprogramming towards Multipotency without Activating Cell Proliferation (2016)
  • Author(s): 伊藤尚文
  • Organizer: The 4th Ribosome Meeting
  • Place of Presentation: 大阪府高槻市、大阪医科大学
  • Year and Date: 2016-09-17
• [Presentation] Ribosome Incorporation into Somatic Cells Impedes Proliferation in Conjunction with Conferring Differentiability into All Three Germ-Layer Lineages (2016)
  • Author(s): 太田訓正
  • Organizer: RNA JAPAN 2016
  • Place of Presentation: 京都市、国立京都国際会館
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Ribosomes covert human fibroblasts into mutipotent cells (2016)
  • Author(s): 太田訓正
  • Organizer: JSDB Special Symposium
  • Place of Presentation: 東京都文京区、東京大学
  • Year and Date: 2016-06-01
  • Int'l Joint Research
• [Presentation] Ribosomes convert human fibroblasts into multipotent cells (2016)
  • Author(s): 太田訓正
  • Organizer: 第14回 幹細胞シンポジウム
  • Place of Presentation: 兵庫県淡路市、淡路夢舞台国際会議場
  • Year and Date: 2016-05-20
• [Presentation] 発酵菌による細胞リプログラミング機構の解明 (2016)
  • Author(s): 太田訓正
  • Organizer: 発酵研究所第10回助成研究報告会
  • Place of Presentation: 大阪府吹田市、千里ライフサイエンスセンター
  • Invited
• [Remarks] 熊本大学 大学院生命科学研究部 神経分化学教室
  • URL: http://srv02.medic.kumamoto-u.ac.jp/dept/devneuro/