Common developmental mechanisms between somatic cells and zygotes
Project/Area Number |
16K14742
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
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Research Institution | Tokyo Metropolitan University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 細胞分化 / 分化全能性 / 体細胞 / 受精卵 / イネ / 物質導入 / 微量オーム解析 / 植物 |
Outline of Final Research Achievements |
In the study, the identification of the proliferative protoplasts among somatic protoplasts isolated from rice callus was conducted. Through the establishment of modified methods for protoplast isolation and culture, we successfully traced the initial developmental profiles of isolated somatic protoplasts, and it was shown that approximate 3.5% of isolated/cultured protoplasts possess proliferative ability. These suggest that the single cell level analysis for proliferative and non-proliferative protoplasts is possible to elucidate the molecular mechanisms of totipotency in plant somatic cells. Moreover, comparison of transcriptome between proliferating protoplasts and zygotes, the natural totipotent cell, is also available to estimate the common cellular mechanisms in somatic and zygotic cellular development. To broaden the scope of investigations using these cells, a procedure for delivery of macromolecules, such as DNA and proteins, into these cells was also established.
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Academic Significance and Societal Importance of the Research Achievements |
一般に被子植物の体細胞は分化全能性をもつとされているが、体細胞組織を構成する細胞群から分化全能性を保持している細胞を特定し、解析することは困難であった。本研究では、イネカルスから調製したプロトプラストから増殖性プロトプラストを特定する手法を確立した。さらに、それら増殖性プロトプラストや受精卵といった全能性をもつ細胞の超微量オーム解析やそれら細胞への物質導入系を可能にし、植物細胞の分化全能性機構の解明に向けた基盤を確立した。
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Expression of genes from paternal alleles in rice zygotes and involvement of OsASGR-BBML1 in initiation of zygotic development2019
Author(s)
Rahman, H., Toda, E., Kobayashi, M., Kudo, T., Koshimizu, S., Takahara, M., Iwami, M., Watanabe, Y., Sekimoto, H., Yano, K., Okamoto, T.
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Journal Title
Plant Cell Physiol.
Volume: 60
Issue: 4
Pages: 725-737
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] DNA demethylation is initiated in the central cells of Arabidopsis and rice.2016
Author(s)
Park K., Kim Y., Vickers M., Park JS., Hyun Y., Okamoto T., Zilberman D., Fischer R., Feng X., Choi Y., Scholten S.
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Journal Title
Proc Natl Acad Sci USA
Volume: 113
Issue: 52
Pages: 15138-15143
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Development of gene expression and genome editing systems in rice egg cells and zygotes by directdelivery of macromolecules2018
Author(s)
Okamoto, T., Toda E., T., Koiso, N., Rahman MH., Takebayashi, A., Ichikawa, M., Kiba, T., Osakabe, Y., Osakabe, T., Sakakibara, H., Kato, N.
Organizer
25th International Congress on Sexual Plant Reproduction
Related Report
Int'l Joint Research
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[Presentation] Genome editing in rice by direct delivery of preassembled CRISPR-Cas9 vectors or ribonucleoproteins into zygotes.2018
Author(s)
Okamoto, T., Toda E., Koiso, N., Takebayashi, A., Ichikawa, M., Kiba, T., Osakabe, Y., Osakabe, T., Sakakibara, H., Kato, N.
Organizer
International Association for Plant Biotechnology Congress 2018
Related Report
Int'l Joint Research
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[Presentation] Effects of imbalanced parental genome ratio on zygotic development and possible function of genes expressing in zygotes with paternal allele-specific manner2017
Author(s)
Toda, E., Kobayashi, M., Takahara, M., Ohnishi Y., Rahman, H., Watanabe, Y., Iwami M., Sekimoto, H., Yano, K., Okamoto, T.
Organizer
Cold Spring Harbor Asia Conference on Plant Cell and Developmental Biology
Related Report
Int'l Joint Research / Invited
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