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Analysis of salicylic acid response in a single plant cell using microfluidics

Research Project

Project/Area Number 16K14750
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Tsukuba

Principal Investigator

BETSUYAKU Shigeyuki  筑波大学, 生命環境系, 准教授 (80588228)

Co-Investigator(Renkei-kenkyūsha) TAKINOUE Masahiro  東京工業大学, 大学院総合理工学研究科, 准教授 (20511249)
KAMIYA Koki  神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (70612315)
TABATA Kazuhito  東京大学, 大学院工学研究科, 准教授 (50403001)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords植物免疫 / レポーター植物 / MEMS / サリチル酸 / 植物ホルモン応答 / イメージング / 一細胞解析 / 植物微生物相互作用 / マイクロ流体デバイス
Outline of Final Research Achievements

To understand plant cellular response against salicylic acid (SA), a hormone required for plant immune response, we aimed to establish a system to analyze the SA response at the single cell level using microfluidic devices and the protoplast derived from Arabidopsis transgenic plants carrying YFP-NLS under the control of the SA-responsive PR1 promoter (pPR1 plants). While we established the 2nd generation pPR1 plants that should enable us to perform genetic analysis as well as highly sensitive imaging, we also conditioned microfluidics-based single cell analysis using the 1st generation pPR1 plants with YFP-NLS as a reporter. In this study, the protoplast from the 1st generation pPR1 plants did not show any YFP signal in response to SA treatment, although the leaf cells of the same pPR1 plant exhibited YFP signal clearly after SA treatment. We are currently investigating the reason of this unresponsiveness only found in protoplasts.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2018 2016 Other

All Presentation (3 results) (of which Invited: 1 results) Remarks (2 results)

  • [Presentation] イメージングによるシロイヌナズナ免疫システムの理解に向けて2018

    • Author(s)
      別役重之・別役恵理子・石賀貴子・石賀康博・野村暢彦
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Towards imaging-based understanding of immune responses in Arabidopsis2018

    • Author(s)
      Betsuyaku, S., Betsuyaku, E., Ishiga, T., Ishiga, Y., Nomura, N
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物免疫システムの時空間的動態を観る2016

    • Author(s)
      別役重之
    • Organizer
      日本植物学会第80回大会(シンポジウム植物ー微生物の見えない相互作用を観る:現在と未来)
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Remarks] 研究室HP

    • URL

      http://www.envr.tsukuba.ac.jp/~microbio/index.html

    • Related Report
      2016 Research-status Report
  • [Remarks] 大学個人紹介HP

    • URL

      http://www.trios.tsukuba.ac.jp/researcher/0000003906

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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