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Molecular mechanisms for redox homeostasis studied by Chlamydomonas phototaxis

Research Project

Project/Area Number 16K14752
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionTokyo Institute of Technology

Principal Investigator

Wakabayashi Ken-ichi  東京工業大学, 科学技術創成研究院, 准教授 (80420248)

Research Collaborator Hisabori Toru  
Yoshida Keisuke  
Ueki Noriko  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords走光性 / レドックス / 鞭毛・繊毛 / 緑藻 / クラミドモナス / 光合成 / 鞭毛
Outline of Final Research Achievements

Redox homeostasis maintains the cellular redox (reduction-oxidation) balance moderately reduced. However, changes in respiratory and photosynthetic activities can lead to oxidized- or excessively reduced state. We succeeded in the isolation of a mutant in which the relationship between phototaxis and intracellular redox state is disrupted in the green alga Chlamydomonas; normally, oxidized cells shows positive phototaxis and reduced cells show negative phototaxis. Those mutants show positive phototaxis even when they are reduced. These strains had an abnormality in which photosynthetic activity decreased. Unfortunately, we were not able to identify the causative gene during this period, but we were able to find an interesting phenomenon in which events occurring in chloroplasts define the direction of cell movement. In the future, the ministry will expedite efforts to identify the genes responsible for the disease.

Academic Significance and Societal Importance of the Research Achievements

学術的には、葉緑体チラコイド膜で起きている現象(還元力伝達の遅滞)が、細胞全体が示す運動方向の異常につながるという新奇現象を見出したことが大きな意義である。この分子メカニズムを明らかにすることができれば、野外における有用藻類培養の効率化や、有害藻類の駆除などに応用できる可能性がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (41 results)

All 2019 2018 2017 2016 Other

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (28 results) (of which Int'l Joint Research: 11 results,  Invited: 9 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Detergent-Extracted Volvox model exhibits an anterior-posterior gradient in flagellar Ca2+ sensitivity2018

    • Author(s)
      Ueki N, Wakabayashi K.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 115 Issue: 5

    • DOI

      10.1073/pnas.1715489115

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Assessment of the flagellar redox potential in Chlamydomonas reinhardtii using a redox-sensitive fluorescent protein, Oba-Qc.2018

    • Author(s)
      Sugiura K, Nishimaki Y, Owa M, Hisabori T, Wakabayashi K.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 503 Pages: 2083-2088

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thioredoxin regulates G6PDH activity by changing redox states of OpcA in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 71202018

    • Author(s)
      Mihara S, Wakao H, Yoshida K, Higo A, Sugiura K, Tsuchiya A, Nomata J, Wakabayashi KI, Hisabori T.
    • Journal Title

      Biochem J.

      Volume: 475 Issue: 6 Pages: 1091-1105

    • DOI

      10.1042/bcj20170869

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Phototaxis Assay for Chlamydomonas reinhardtii2017

    • Author(s)
      Ueki Noriko、Wakabayashi Ken-ichi
    • Journal Title

      BIO-PROTOCOL

      Volume: 7 Issue: 12

    • DOI

      10.21769/bioprotoc.2356

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 緑藻クラミドモナスの走光性と細胞レンズ効果 ―藻類の「眼」の赤い色の役割―2017

    • Author(s)
      植木紀子、若林憲一
    • Journal Title

      化学と生物

      Volume: 55 Pages: 366-368

    • NAID

      130006742901

    • Related Report
      2017 Research-status Report
  • [Journal Article] 緑藻クラミドモナスの鞭毛運動と光行動2017

    • Author(s)
      若林憲一、中島昌子、井手隆広、植木紀子
    • Journal Title

      日本プランクトン学会報

      Volume: 64 Pages: 61-66

    • NAID

      130007609952

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] A New Morphological Type of Volvox from Japanese Large Lakes and Recent Divergence of this Type and V. ferrisii in Two Different Freshwater Habitats.2016

    • Author(s)
      Nozaki H, Ueki N, Isaka N, Saigo T, Yamamoto K, Matsuzaki R, Takahashi F, Wakabayashi K, Kawachi M.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 11 Pages: e0167148-e0167148

    • DOI

      10.1371/journal.pone.0167148

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii2016

    • Author(s)
      Ide, T., Mochiji, S., Ueki, N., Yamaguchi, K., Shigenobu, S., Hirono, M., Wakabayashi, K.
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 7 Pages: 379-385

    • DOI

      10.1016/j.bbrep.2016.07.016

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016

    • Author(s)
      Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 印刷中 Issue: 19 Pages: 5266-5304

    • DOI

      10.1073/pnas.1525538113

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Analysis of a mutant showing strong positive phototaxis in the green alga Chlamydomonas reinhardtii2019

    • Author(s)
      法元悠一郎、岡島圭佑、中島昌子、植木紀子、山口勝司、重信秀治、皆川純、久堀徹、若林憲一
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 緑藻テトラバエナの光行動2019

    • Author(s)
      丹野明日翔、新垣陽子、得津隆太郎、皆川純、吉村建二郎、野崎久義、久堀徹、若林憲一
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2018 Annual Research Report
  • [Presentation] 微細藻類の鞭毛運動と光行動2018

    • Author(s)
      若林憲一
    • Organizer
      ニコンイメージングセンター学術講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] More (or Less) Light: Phototaxis in Chlamydomonas and Volvox2018

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      Gordon Research Conference "Photosensory Receptors and Signal Transduction"
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] More (or less) light: Phototaxis in Chlamydomonas and Volvox2018

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      Gordon Research Conference “Photo-sensing and signaling cascade”
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] プラナリア繊毛におけるダイニンサブユニット機能の解析2018

    • Author(s)
      久次史花, Ramila Patel-King, 久堀徹, Stephen M. King, 若林憲一
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2017 Research-status Report
  • [Presentation] ボルボックス目緑藻テトラバエナの光行動2018

    • Author(s)
      丹野明日翔, 中島昌子, 植木紀子, 新垣陽子, 吉村建二郎, 野崎久義, 久堀徹, 若林憲一
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2017 Research-status Report
  • [Presentation] クラミドモナスの回避反応と重力走性におけるTRPチャネルの役割2018

    • Author(s)
      吉田晃子、吉田愛美、安齋幸祐、若林憲一、吉村建二郎
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2017 Research-status Report
  • [Presentation] クラミドモナスのγ-tubulin 優性突然変異 bld132017

    • Author(s)
      中澤友紀 、堀井麻央 、渡辺早紀 、大月萌子 、苗加彰 、若林憲一 、廣野雅文
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      神戸・神戸国際会議場
    • Year and Date
      2017-01-06
    • Related Report
      2016 Research-status Report
  • [Presentation] クラミドモナスにおける走光性の正負と「利き鞭毛」の関係2017

    • Author(s)
      中島昌子 、植木紀子 、若林憲一
    • Organizer
      生体運動研究合同班会議
    • Place of Presentation
      神戸・神戸国際会議場
    • Year and Date
      2017-01-06
    • Related Report
      2016 Research-status Report
  • [Presentation] Flagellar regulation for phototactic turning in Chlamydomonas and Volvox2017

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      International Workshop Dynein 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional analysis of outer-arm dynein subunits in the planarian Schmidtea mediterranea2017

    • Author(s)
      Ayaka Kyuji, Ramila Patel-King, Toru Hisabori, Ken-ichi Wakabayashi, Stephen M. King
    • Organizer
      International Workshop Dynein 2017
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Photomovements in Chlamydomonas and Volvox2017

    • Author(s)
      Ken-ichi Wakabayashi
    • Organizer
      International Symposium on "Harmonized supramolecular motility machinery and its diversity"
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ボルボックス目緑藻テトラバエナの光行動能の検定2017

    • Author(s)
      丹野 明日翔, 中島 昌子, 植木 紀子, 新垣 陽子, 吉村 建二郎, 野崎 久義, 久堀 徹, 若林 憲一
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] クラミドモナス走光性におけるカロテノイドの役割2017

    • Author(s)
      若林憲一
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Anabaena sp. PCC 7120のチオレドキシン還元酵素およびチオレドキシンの機能解析2017

    • Author(s)
      見原 翔子 吉田 啓亮 若林 憲一 久堀 徹
    • Organizer
      第58回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 緑藻の光行動における鞭毛運動調節:クラミドモナスとボルボックス2017

    • Author(s)
      植木紀子、若林憲一
    • Organizer
      第58回日本植物生理学会年会
    • Related Report
      2017 Research-status Report 2016 Research-status Report
  • [Presentation] クラミドモナスのγ-tubulin 優性突然変異 bld132017

    • Author(s)
      中澤友紀、堀井麻央、渡辺早紀、大月萌子、苗加彰、若林憲一、廣野雅文
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2017 Research-status Report
  • [Presentation] クラミドモナスにおける走光性の正負と「利き鞭毛」の関係2017

    • Author(s)
      中島昌子、植木紀子、若林憲一
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2017 Research-status Report
  • [Presentation] ボルボックスのカルシウム依存的繊毛打逆転と走光性2017

    • Author(s)
      植木紀子、若林憲一
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2017 Research-status Report 2016 Research-status Report
  • [Presentation] Photo-behavior in green algae: how light modulates flagellar motility2017

    • Author(s)
      Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
    • Organizer
      光合成科学:エネルギーとバイオマス
    • Place of Presentation
      横浜・東工大すずかけホール
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Modulation of flagellar motility during phototactic turning in Chlamydomonas2016

    • Author(s)
      Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
    • Organizer
      The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
    • Place of Presentation
      神戸・理研CDB
    • Year and Date
      2016-11-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The molecular toolbox for building axonemal microtubules2016

    • Author(s)
      Mikito Owa, Takayuki Uchihashi, Haruaki Yanagisawa, Takashi Yamano, Hiro Iguchi, Hideya Fukuzawa, Ken-ichi Wakabayashi, Toshio Ando, Masahide Kikkawa
    • Organizer
      The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
    • Place of Presentation
      神戸・理研CDB
    • Year and Date
      2016-11-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 緑藻クラミドモナスの走光性発現メカニズム2016

    • Author(s)
      若林憲一
    • Organizer
      遺伝研研究会「水圏微生物のエネルギー利用戦略とその相互作用に関する研究会」
    • Place of Presentation
      三島・遺伝研
    • Year and Date
      2016-11-03
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Visualization and quantification of the redox potential in the Chlamydomonas flagella2016

    • Author(s)
      Yuta Nishimaki, Kazunori Sugiura, Toru Hisabori, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016

    • Author(s)
      Noriko Ueki, Takahiro Ide, Shota Mochiji, Yuki Kobayashi, Ryutaro Tokutsu, Norikazu Ohnishi, Katushi Yamaguchi, Shuji Shigenobu, Kan Tanaka, Jun Minagawa, Toru Hisabori, Masafumi Hirono, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Isolation of a non-phototactic Chlamydomonas mutant that shows alternate flagellar beatings2016

    • Author(s)
      Akinori Koitabashi, Masako Nakajima, Noriko Ueki, Kogiku Shiba, Takashi Ymano, Hideya Fukuzawa, Inaba Kazuo, Toru Hisabori, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii2016

    • Author(s)
      Takahiro Ide, Shota Mochuji, Noriko Ueki, Katsushi Yamaguchi, Shuji Shigenobu, Masafumi Hirono, Ken-ichi Wakabayashi
    • Organizer
      The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Place of Presentation
      京都・京都国際会館
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] Dyneins: Structure, Biology and Disease 2nd ed.2017

    • Author(s)
      Ueki N, Wakabayashi, K. (共著、Stephen M. King編)
    • Total Pages
      1147
    • Publisher
      Elsevier Inc.
    • Related Report
      2017 Research-status Report
  • [Remarks] 久堀若林研究室ウェブサイト

    • URL

      http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 久堀・若林研究室ホームページ

    • URL

      http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html

    • Related Report
      2017 Research-status Report
  • [Remarks] 久堀・若林研究室ウェブサイト

    • URL

      http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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