Molecular mechanisms for redox homeostasis studied by Chlamydomonas phototaxis
| Project/Area Number |
16K14752
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Plant molecular biology/Plant physiology
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
Hisabori Toru
Yoshida Keisuke
Ueki Noriko
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 走光性 / レドックス / 鞭毛・繊毛 / 緑藻 / クラミドモナス / 光合成 / 鞭毛 |
|---|
| Outline of Final Research Achievements |
Redox homeostasis maintains the cellular redox (reduction-oxidation) balance moderately reduced. However, changes in respiratory and photosynthetic activities can lead to oxidized- or excessively reduced state. We succeeded in the isolation of a mutant in which the relationship between phototaxis and intracellular redox state is disrupted in the green alga Chlamydomonas; normally, oxidized cells shows positive phototaxis and reduced cells show negative phototaxis. Those mutants show positive phototaxis even when they are reduced. These strains had an abnormality in which photosynthetic activity decreased. Unfortunately, we were not able to identify the causative gene during this period, but we were able to find an interesting phenomenon in which events occurring in chloroplasts define the direction of cell movement. In the future, the ministry will expedite efforts to identify the genes responsible for the disease.
|
| Academic Significance and Societal Importance of the Research Achievements |
学術的には、葉緑体チラコイド膜で起きている現象（還元力伝達の遅滞）が、細胞全体が示す運動方向の異常につながるという新奇現象を見出したことが大きな意義である。この分子メカニズムを明らかにすることができれば、野外における有用藻類培養の効率化や、有害藻類の駆除などに応用できる可能性がある。
|
Report
(4 results)
Research Products
(41 results)
-
-
-
-
-
-
-
-
[Journal Article] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii2016
Author(s)
Ide, T., Mochiji, S., Ueki, N., Yamaguchi, K., Shigenobu, S., Hirono, M., Wakabayashi, K.
-
Journal Title
Biochemistry and Biophysics Reports
Volume: 7
Pages: 379-385
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016
Author(s)
Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
-
Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 印刷中
Issue: 19
Pages: 5266-5304
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] The molecular toolbox for building axonemal microtubules2016
Author(s)
Mikito Owa, Takayuki Uchihashi, Haruaki Yanagisawa, Takashi Yamano, Hiro Iguchi, Hideya Fukuzawa, Ken-ichi Wakabayashi, Toshio Ando, Masahide Kikkawa
Organizer
The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
Place of Presentation
神戸・理研CDB
Year and Date
2016-11-27
Related Report
Int'l Joint Research
-
-
-
[Presentation] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016
Author(s)
Noriko Ueki, Takahiro Ide, Shota Mochiji, Yuki Kobayashi, Ryutaro Tokutsu, Norikazu Ohnishi, Katushi Yamaguchi, Shuji Shigenobu, Kan Tanaka, Jun Minagawa, Toru Hisabori, Masafumi Hirono, Ken-ichi Wakabayashi
Organizer
The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
Place of Presentation
京都・京都国際会館
Year and Date
2016-06-26
Related Report
Int'l Joint Research
-
-
-
-
-
-
