Molecular mechanisms for redox homeostasis studied by Chlamydomonas phototaxis

• Project/Area Number: 16K14752
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Wakabayashi Ken-ichi 東京工業大学, 科学技術創成研究院, 准教授 (80420248)
• Research Collaborator: Hisabori Toru ; Yoshida Keisuke ; Ueki Noriko
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 走光性 / レドックス / 鞭毛・繊毛 / 緑藻 / クラミドモナス / 光合成 / 鞭毛

Outline of Final Research Achievements

Redox homeostasis maintains the cellular redox (reduction-oxidation) balance moderately reduced. However, changes in respiratory and photosynthetic activities can lead to oxidized- or excessively reduced state. We succeeded in the isolation of a mutant in which the relationship between phototaxis and intracellular redox state is disrupted in the green alga Chlamydomonas; normally, oxidized cells shows positive phototaxis and reduced cells show negative phototaxis. Those mutants show positive phototaxis even when they are reduced. These strains had an abnormality in which photosynthetic activity decreased. Unfortunately, we were not able to identify the causative gene during this period, but we were able to find an interesting phenomenon in which events occurring in chloroplasts define the direction of cell movement. In the future, the ministry will expedite efforts to identify the genes responsible for the disease.

Academic Significance and Societal Importance of the Research Achievements

学術的には、葉緑体チラコイド膜で起きている現象（還元力伝達の遅滞）が、細胞全体が示す運動方向の異常につながるという新奇現象を見出したことが大きな意義である。この分子メカニズムを明らかにすることができれば、野外における有用藻類培養の効率化や、有害藻類の駆除などに応用できる可能性がある。

Research Products

• [Journal Article] Detergent-Extracted Volvox model exhibits an anterior-posterior gradient in flagellar Ca2+ sensitivity (2018)
  • Author(s): Ueki N, Wakabayashi K.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 115 Issue: 5
  • DOI: 10.1073/pnas.1715489115
  • Peer Reviewed
• [Journal Article] Assessment of the flagellar redox potential in Chlamydomonas reinhardtii using a redox-sensitive fluorescent protein, Oba-Qc. (2018)
  • Author(s): Sugiura K, Nishimaki Y, Owa M, Hisabori T, Wakabayashi K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 503 Pages: 2083-2088
  • Peer Reviewed
• [Journal Article] Thioredoxin regulates G6PDH activity by changing redox states of OpcA in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2018)
  • Author(s): Mihara S, Wakao H, Yoshida K, Higo A, Sugiura K, Tsuchiya A, Nomata J, Wakabayashi KI, Hisabori T.
  • Journal Title: Biochem J. Volume: 475 Issue: 6 Pages: 1091-1105
  • DOI: 10.1042/bcj20170869
  • Peer Reviewed
• [Journal Article] Phototaxis Assay for Chlamydomonas reinhardtii (2017)
  • Author(s): Ueki Noriko、Wakabayashi Ken-ichi
  • Journal Title: BIO-PROTOCOL Volume: 7 Issue: 12
  • DOI: 10.21769/bioprotoc.2356
  • Peer Reviewed / Open Access
• [Journal Article] 緑藻クラミドモナスの走光性と細胞レンズ効果 ―藻類の「眼」の赤い色の役割― (2017)
  • Author(s): 植木紀子、若林憲一
  • Journal Title: 化学と生物 Volume: 55 Pages: 366-368
  • NAID: 130006742901
• [Journal Article] 緑藻クラミドモナスの鞭毛運動と光行動 (2017)
  • Author(s): 若林憲一、中島昌子、井手隆広、植木紀子
  • Journal Title: 日本プランクトン学会報 Volume: 64 Pages: 61-66
  • NAID: 130007609952
  • Peer Reviewed
• [Journal Article] A New Morphological Type of Volvox from Japanese Large Lakes and Recent Divergence of this Type and V. ferrisii in Two Different Freshwater Habitats. (2016)
  • Author(s): Nozaki H, Ueki N, Isaka N, Saigo T, Yamamoto K, Matsuzaki R, Takahashi F, Wakabayashi K, Kawachi M.
  • Journal Title: PLoS One Volume: 11 Issue: 11 Pages: e0167148-e0167148
  • DOI: 10.1371/journal.pone.0167148
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii (2016)
  • Author(s): Ide, T., Mochiji, S., Ueki, N., Yamaguchi, K., Shigenobu, S., Hirono, M., Wakabayashi, K.
  • Journal Title: Biochemistry and Biophysics Reports Volume: 7 Pages: 379-385
  • DOI: 10.1016/j.bbrep.2016.07.016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii (2016)
  • Author(s): Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 印刷中 Issue: 19 Pages: 5266-5304
  • DOI: 10.1073/pnas.1525538113
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Analysis of a mutant showing strong positive phototaxis in the green alga Chlamydomonas reinhardtii (2019)
  • Author(s): 法元悠一郎、岡島圭佑、中島昌子、植木紀子、山口勝司、重信秀治、皆川純、久堀徹、若林憲一
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] 緑藻テトラバエナの光行動 (2019)
  • Author(s): 丹野明日翔、新垣陽子、得津隆太郎、皆川純、吉村建二郎、野崎久義、久堀徹、若林憲一
  • Organizer: 生体運動研究合同班会議
• [Presentation] 微細藻類の鞭毛運動と光行動 (2018)
  • Author(s): 若林憲一
  • Organizer: ニコンイメージングセンター学術講演会
  • Invited
• [Presentation] More (or Less) Light: Phototaxis in Chlamydomonas and Volvox (2018)
  • Author(s): Ken-ichi Wakabayashi
  • Organizer: Gordon Research Conference "Photosensory Receptors and Signal Transduction"
  • Int'l Joint Research / Invited
• [Presentation] More (or less) light: Phototaxis in Chlamydomonas and Volvox (2018)
  • Author(s): Ken-ichi Wakabayashi
  • Organizer: Gordon Research Conference “Photo-sensing and signaling cascade”
  • Int'l Joint Research / Invited
• [Presentation] プラナリア繊毛におけるダイニンサブユニット機能の解析 (2018)
  • Author(s): 久次史花, Ramila Patel-King, 久堀徹, Stephen M. King, 若林憲一
  • Organizer: 生体運動研究合同班会議
• [Presentation] ボルボックス目緑藻テトラバエナの光行動 (2018)
  • Author(s): 丹野明日翔, 中島昌子, 植木紀子, 新垣陽子, 吉村建二郎, 野崎久義, 久堀徹, 若林憲一
  • Organizer: 生体運動研究合同班会議
• [Presentation] クラミドモナスの回避反応と重力走性におけるTRPチャネルの役割 (2018)
  • Author(s): 吉田晃子、吉田愛美、安齋幸祐、若林憲一、吉村建二郎
  • Organizer: 生体運動研究合同班会議
• [Presentation] クラミドモナスのγ-tubulin 優性突然変異 bld13 (2017)
  • Author(s): 中澤友紀 、堀井麻央 、渡辺早紀 、大月萌子 、苗加彰 、若林憲一 、廣野雅文
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 神戸・神戸国際会議場
  • Year and Date: 2017-01-06
• [Presentation] クラミドモナスにおける走光性の正負と「利き鞭毛」の関係 (2017)
  • Author(s): 中島昌子 、植木紀子 、若林憲一
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 神戸・神戸国際会議場
  • Year and Date: 2017-01-06
• [Presentation] Flagellar regulation for phototactic turning in Chlamydomonas and Volvox (2017)
  • Author(s): Ken-ichi Wakabayashi
  • Organizer: International Workshop Dynein 2017
  • Int'l Joint Research / Invited
• [Presentation] Functional analysis of outer-arm dynein subunits in the planarian Schmidtea mediterranea (2017)
  • Author(s): Ayaka Kyuji, Ramila Patel-King, Toru Hisabori, Ken-ichi Wakabayashi, Stephen M. King
  • Organizer: International Workshop Dynein 2017
  • Invited
• [Presentation] Photomovements in Chlamydomonas and Volvox (2017)
  • Author(s): Ken-ichi Wakabayashi
  • Organizer: International Symposium on "Harmonized supramolecular motility machinery and its diversity"
  • Int'l Joint Research / Invited
• [Presentation] ボルボックス目緑藻テトラバエナの光行動能の検定 (2017)
  • Author(s): 丹野 明日翔, 中島 昌子, 植木 紀子, 新垣 陽子, 吉村 建二郎, 野崎 久義, 久堀 徹, 若林 憲一
  • Organizer: 日本植物学会第81回大会
• [Presentation] クラミドモナス走光性におけるカロテノイドの役割 (2017)
  • Author(s): 若林憲一
  • Organizer: 日本植物学会第81回大会
  • Invited
• [Presentation] Anabaena sp. PCC 7120のチオレドキシン還元酵素およびチオレドキシンの機能解析 (2017)
  • Author(s): 見原 翔子 吉田 啓亮 若林 憲一 久堀 徹
  • Organizer: 第58回日本植物生理学会年会
• [Presentation] 緑藻の光行動における鞭毛運動調節：クラミドモナスとボルボックス (2017)
  • Author(s): 植木紀子、若林憲一
  • Organizer: 第58回日本植物生理学会年会
• [Presentation] クラミドモナスのγ-tubulin 優性突然変異 bld13 (2017)
  • Author(s): 中澤友紀、堀井麻央、渡辺早紀、大月萌子、苗加彰、若林憲一、廣野雅文
  • Organizer: 生体運動研究合同班会議
• [Presentation] クラミドモナスにおける走光性の正負と「利き鞭毛」の関係 (2017)
  • Author(s): 中島昌子、植木紀子、若林憲一
  • Organizer: 生体運動研究合同班会議
• [Presentation] ボルボックスのカルシウム依存的繊毛打逆転と走光性 (2017)
  • Author(s): 植木紀子、若林憲一
  • Organizer: 生体運動研究合同班会議
• [Presentation] Photo-behavior in green algae: how light modulates flagellar motility (2017)
  • Author(s): Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
  • Organizer: 光合成科学：エネルギーとバイオマス
  • Place of Presentation: 横浜・東工大すずかけホール
  • Int'l Joint Research / Invited
• [Presentation] Modulation of flagellar motility during phototactic turning in Chlamydomonas (2016)
  • Author(s): Masako Nakajima, Noriko Ueki, Ken-ichi Wakabayashi
  • Organizer: The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
  • Place of Presentation: 神戸・理研CDB
  • Year and Date: 2016-11-27
  • Int'l Joint Research
• [Presentation] The molecular toolbox for building axonemal microtubules (2016)
  • Author(s): Mikito Owa, Takayuki Uchihashi, Haruaki Yanagisawa, Takashi Yamano, Hiro Iguchi, Hideya Fukuzawa, Ken-ichi Wakabayashi, Toshio Ando, Masahide Kikkawa
  • Organizer: The 28th CDB Meeting: Cilia and Centrosomes: Current Advances and Future Directions
  • Place of Presentation: 神戸・理研CDB
  • Year and Date: 2016-11-27
  • Int'l Joint Research
• [Presentation] 緑藻クラミドモナスの走光性発現メカニズム (2016)
  • Author(s): 若林憲一
  • Organizer: 遺伝研研究会「水圏微生物のエネルギー利用戦略とその相互作用に関する研究会」
  • Place of Presentation: 三島・遺伝研
  • Year and Date: 2016-11-03
  • Invited
• [Presentation] Visualization and quantification of the redox potential in the Chlamydomonas flagella (2016)
  • Author(s): Yuta Nishimaki, Kazunori Sugiura, Toru Hisabori, Ken-ichi Wakabayashi
  • Organizer: The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii (2016)
  • Author(s): Noriko Ueki, Takahiro Ide, Shota Mochiji, Yuki Kobayashi, Ryutaro Tokutsu, Norikazu Ohnishi, Katushi Yamaguchi, Shuji Shigenobu, Kan Tanaka, Jun Minagawa, Toru Hisabori, Masafumi Hirono, Ken-ichi Wakabayashi
  • Organizer: The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Isolation of a non-phototactic Chlamydomonas mutant that shows alternate flagellar beatings (2016)
  • Author(s): Akinori Koitabashi, Masako Nakajima, Noriko Ueki, Kogiku Shiba, Takashi Ymano, Hideya Fukuzawa, Inaba Kazuo, Toru Hisabori, Ken-ichi Wakabayashi
  • Organizer: The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Presentation] Identification of the agg1 mutation responsible for negative phototaxis in a "wild-type" strain of Chlamydomonas reinhardtii (2016)
  • Author(s): Takahiro Ide, Shota Mochuji, Noriko Ueki, Katsushi Yamaguchi, Shuji Shigenobu, Masafumi Hirono, Ken-ichi Wakabayashi
  • Organizer: The 17th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2016-06-26
  • Int'l Joint Research
• [Book] Dyneins: Structure, Biology and Disease 2nd ed. (2017)
  • Author(s): Ueki N, Wakabayashi, K. (共著、Stephen M. King編）
  • Total Pages: 1147
  • Publisher: Elsevier Inc.
• [Remarks] 久堀若林研究室ウェブサイト
  • URL: http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html
• [Remarks] 久堀・若林研究室ホームページ
  • URL: http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html
• [Remarks] 久堀・若林研究室ウェブサイト
  • URL: http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html