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Novel methods for live-imaging and chemical screening to reveal the molecular mechanism of plant asymmetric cell division

Research Project

Project/Area Number 16K14753
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagoya University

Principal Investigator

Ueda Minako  名古屋大学, 理学研究科(WPI), 特任講師 (20598726)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords植物受精卵 / ライブイメージング / 化合物スクリーニング / 植物 / 発生 / 細胞極性 / イメージング
Outline of Final Research Achievements

In most flowering plants, the asymmetric cell division of the zygote is the initial step in establishing the apical-basal axis. In Arabidopsis thaliana, the zygote is polarized, possessing the nucleus at the apical tip in the elongated cell. In spite of its obvious asymmetry, the real-time dynamics of the zygote polarization steps was poorly understood. Therefore we established a live-cell imaging system to visualize intracellular dynamics of the zygote through the ovule (Ueda and Berger, 2018). By combining this system with various specific inhibitors and mutants, we assessed the dynamics and roles of vacuoles in the zygote polarization (Kimata et. al., 2019). We also have found novel compounds, which effectively inhibit the zygotic division (in manuscript preparation).

Academic Significance and Societal Importance of the Research Achievements

研究代表者らは、世界で初めて、植物の受精卵が極性化する動態をリアルタイムで観察することに成功した。さらに、これまで単なる水袋だと考えられてきた液胞のダイナミックな動きが、受精卵の非対称分裂だけでなく、その後の形作りにも必須であることも突き止めた。ほとんどの植物において、受精卵は大きな液胞を持つことから、本研究が明らかにした仕組みは、植物に共通した普遍的な機構であると期待される。液胞は植物だけでなく、真菌類にまで保存された基本的な細胞内小器官のため、植物の形が複雑に進化してきた経緯の理解や、農業技術への応用など、さまざまな分野への展開が期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (20 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 6 results) Remarks (1 results)

  • [Int'l Joint Research] Gregor Mendel Institute(オーストリア)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Freiburg University(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Freiburg University(ドイツ)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] Gregor Mendel Institute(オーストリア)

    • Related Report
      2017 Research-status Report
  • [Journal Article] New cues for body axis formation in plant embryos2019

    • Author(s)
      Ueda Minako、Berger Frederic
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 47 Pages: 16-21

    • DOI

      10.1016/j.pbi.2018.08.005

    • NAID

      120006650158

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Polar vacuolar distribution is essential for accurate asymmetric division ofArabidopsiszygotes2019

    • Author(s)
      Kimata Yusuke、Kato Takehide、Higaki Takumi、Kurihara Daisuke、Yamada Tomomi、Segami Shoji、Morita Miyo Terao、Maeshima Masayoshi、Hasezawa Seiichiro、Higashiyama Tetsuya、Tasaka Masao、Ueda Minako
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 116 Issue: 6 Pages: 2338-2343

    • DOI

      10.1073/pnas.1814160116

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote2017

    • Author(s)
      Ueda M, Aichinger E, Gong W, Groot E, Verstraeten I, Vu LD, De Smet I, Higashiyama T, Umeda M, Laux T
    • Journal Title

      Genes & Development

      Volume: 31 Issue: 6 Pages: 617-627

    • DOI

      10.1101/gad.292409.116

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in <em>Arabidopsis thaliana</em>2017

    • Author(s)
      Kurihara Daisuke、Kimata Yusuke、Higashiyama Tetsuya、Ueda Minako
    • Journal Title

      Journal of Visualized Experiments

      Volume: なし Issue: 127

    • DOI

      10.3791/55975

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote.2016

    • Author(s)
      Kimata, Y., Higaki, T., Kawashima, T., Kurihara, D., Sato, Y., Yamada, T., Hasezawa, S., Berger, F., Higashiyama, T., and Ueda, M.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 113 Issue: 49 Pages: 14157-14162

    • DOI

      10.1073/pnas.1613979113

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Combination of synthetic chemistry and live-cell imaging identified a rapid cell division inhibitor in tobacco and Arabidopsis thaliana2016

    • Author(s)
      Nambo, M., Kurihara, D., Yamada, T., Nishiwaki-Ohkawa, T., Kadofusa, N., Kimata, Y., Kuwata, K., Umeda, M. and Ueda, M.
    • Journal Title

      Plant Cell Physiol.

      Volume: 57 Issue: 11 Pages: 2255-2268

    • DOI

      10.1093/pcp/pcw140

    • NAID

      120006220249

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] シロイヌナズナ初期胚のライブイメージング2019

    • Author(s)
      植田美那子、栗原大輔、瀬上紹嗣、前島正義、東山哲也
    • Organizer
      植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Live-cell imaging of the axis formation in Arabidopsis zygote2018

    • Author(s)
      Kimata, Y., Ando, N., Kato, T., Higaki, T., Kurihara, D., Yamada, T., Segami, S., Morita, M. T., Maeshima, M., Hasezawa, S., Higashiyama, T., Tasaka, M., Ueda, M.
    • Organizer
      The 25th International Congress on Sexual Plant Reproduction (25th ICSPR)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Live-cell imaging of the intracellular dynamics of Arabidopsis zygote2018

    • Author(s)
      Kimata, Y., Ando, N., Higaki, T., Kurihara, D., Kato, T., Yamada, T., Segami, S., Morita, M. T., Maeshima, M., Hasezawa, S., Higashiyama, T., Tasaka, M., Ueda, M.
    • Organizer
      GARNet2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物の受精卵はどのように極性化するのか?2017

    • Author(s)
      植田美那子,木全祐資,栗原大輔,山田朋美,東山哲也
    • Organizer
      第58会植物生理学会年会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 受精による細胞極性の破壊と再構成; ライブイメージングで迫る受精前後の細胞内変化2017

    • Author(s)
      植田美那子
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Live-cell imaging of the plant axis formation responding to fertilization signals.2017

    • Author(s)
      植田美那子
    • Organizer
      ISIF2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Live-cell imaging of the intracellular dynamics during zygote polarization2017

    • Author(s)
      植田美那子
    • Organizer
      28th ICAR2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Live imaging of microtubule dynamics during plant zygote polarization2017

    • Author(s)
      植田美那子
    • Organizer
      第69回日本細胞生物学会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Live-cell imaging of the intracellular dynamics underlying the zygote polarization in Arabidopsis2016

    • Author(s)
      Yusuke Kimata, Daisuke Kurihara, Takumi Higaki, Tomomi Yamada, Seiichiro Hasezawa, Tetsuya Higashiyama and Minako Ueda
    • Organizer
      27th International Conference on Arabidopsis Research (ICAR2016)
    • Place of Presentation
      慶州 (韓国)
    • Year and Date
      2016-06-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 植物では両親の遺伝子の協力によって子供の形ができる

    • URL

      http://www.itbm.nagoya-u.ac.jp/ja/research/2017/04/GandD-Parents.php

    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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