Novel methods for live-imaging and chemical screening to reveal the molecular mechanism of plant asymmetric cell division

• Project/Area Number: 16K14753
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: Ueda Minako 名古屋大学, 理学研究科(WPI), 特任講師 (20598726)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 植物受精卵 / ライブイメージング / 化合物スクリーニング / 植物 / 発生 / 細胞極性 / イメージング

Outline of Final Research Achievements

In most flowering plants, the asymmetric cell division of the zygote is the initial step in establishing the apical-basal axis. In Arabidopsis thaliana, the zygote is polarized, possessing the nucleus at the apical tip in the elongated cell. In spite of its obvious asymmetry, the real-time dynamics of the zygote polarization steps was poorly understood. Therefore we established a live-cell imaging system to visualize intracellular dynamics of the zygote through the ovule (Ueda and Berger, 2018). By combining this system with various specific inhibitors and mutants, we assessed the dynamics and roles of vacuoles in the zygote polarization (Kimata et. al., 2019). We also have found novel compounds, which effectively inhibit the zygotic division (in manuscript preparation).

Academic Significance and Societal Importance of the Research Achievements

研究代表者らは、世界で初めて、植物の受精卵が極性化する動態をリアルタイムで観察することに成功した。さらに、これまで単なる水袋だと考えられてきた液胞のダイナミックな動きが、受精卵の非対称分裂だけでなく、その後の形作りにも必須であることも突き止めた。ほとんどの植物において、受精卵は大きな液胞を持つことから、本研究が明らかにした仕組みは、植物に共通した普遍的な機構であると期待される。液胞は植物だけでなく、真菌類にまで保存された基本的な細胞内小器官のため、植物の形が複雑に進化してきた経緯の理解や、農業技術への応用など、さまざまな分野への展開が期待できる。

Research Products

• [Int'l Joint Research] Gregor Mendel Institute(オーストリア)
• [Int'l Joint Research] Freiburg University(ドイツ)
• [Int'l Joint Research] Freiburg University(ドイツ)
• [Int'l Joint Research] Gregor Mendel Institute(オーストリア)
• [Journal Article] New cues for body axis formation in plant embryos (2019)
  • Author(s): Ueda Minako、Berger Frederic
  • Journal Title: Current Opinion in Plant Biology Volume: 47 Pages: 16-21
  • DOI: 10.1016/j.pbi.2018.08.005
  • NAID: 120006650158
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Polar vacuolar distribution is essential for accurate asymmetric division ofArabidopsiszygotes (2019)
  • Author(s): Kimata Yusuke、Kato Takehide、Higaki Takumi、Kurihara Daisuke、Yamada Tomomi、Segami Shoji、Morita Miyo Terao、Maeshima Masayoshi、Hasezawa Seiichiro、Higashiyama Tetsuya、Tasaka Masao、Ueda Minako
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 116 Issue: 6 Pages: 2338-2343
  • DOI: 10.1073/pnas.1814160116
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote (2017)
  • Author(s): Ueda M, Aichinger E, Gong W, Groot E, Verstraeten I, Vu LD, De Smet I, Higashiyama T, Umeda M, Laux T
  • Journal Title: Genes & Development Volume: 31 Issue: 6 Pages: 617-627
  • DOI: 10.1101/gad.292409.116
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in <em>Arabidopsis thaliana</em> (2017)
  • Author(s): Kurihara Daisuke、Kimata Yusuke、Higashiyama Tetsuya、Ueda Minako
  • Journal Title: Journal of Visualized Experiments Volume: なし Issue: 127
  • DOI: 10.3791/55975
  • Peer Reviewed
• [Journal Article] Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote. (2016)
  • Author(s): Kimata, Y., Higaki, T., Kawashima, T., Kurihara, D., Sato, Y., Yamada, T., Hasezawa, S., Berger, F., Higashiyama, T., and Ueda, M.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 113 Issue: 49 Pages: 14157-14162
  • DOI: 10.1073/pnas.1613979113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Combination of synthetic chemistry and live-cell imaging identified a rapid cell division inhibitor in tobacco and Arabidopsis thaliana (2016)
  • Author(s): Nambo, M., Kurihara, D., Yamada, T., Nishiwaki-Ohkawa, T., Kadofusa, N., Kimata, Y., Kuwata, K., Umeda, M. and Ueda, M.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 11 Pages: 2255-2268
  • DOI: 10.1093/pcp/pcw140
  • NAID: 120006220249
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] シロイヌナズナ初期胚のライブイメージング (2019)
  • Author(s): 植田美那子、栗原大輔、瀬上紹嗣、前島正義、東山哲也
  • Organizer: 植物生理学会
• [Presentation] Live-cell imaging of the axis formation in Arabidopsis zygote (2018)
  • Author(s): Kimata, Y., Ando, N., Kato, T., Higaki, T., Kurihara, D., Yamada, T., Segami, S., Morita, M. T., Maeshima, M., Hasezawa, S., Higashiyama, T., Tasaka, M., Ueda, M.
  • Organizer: The 25th International Congress on Sexual Plant Reproduction (25th ICSPR)
  • Int'l Joint Research
• [Presentation] Live-cell imaging of the intracellular dynamics of Arabidopsis zygote (2018)
  • Author(s): Kimata, Y., Ando, N., Higaki, T., Kurihara, D., Kato, T., Yamada, T., Segami, S., Morita, M. T., Maeshima, M., Hasezawa, S., Higashiyama, T., Tasaka, M., Ueda, M.
  • Organizer: GARNet2018
  • Int'l Joint Research / Invited
• [Presentation] 植物の受精卵はどのように極性化するのか？ (2017)
  • Author(s): 植田美那子，木全祐資，栗原大輔，山田朋美，東山哲也
  • Organizer: 第58会植物生理学会年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
• [Presentation] 受精による細胞極性の破壊と再構成; ライブイメージングで迫る受精前後の細胞内変化 (2017)
  • Author(s): 植田美那子
  • Organizer: 日本植物学会第81回大会
  • Invited
• [Presentation] Live-cell imaging of the plant axis formation responding to fertilization signals. (2017)
  • Author(s): 植田美那子
  • Organizer: ISIF2017
  • Int'l Joint Research / Invited
• [Presentation] Live-cell imaging of the intracellular dynamics during zygote polarization (2017)
  • Author(s): 植田美那子
  • Organizer: 28th ICAR2017
  • Int'l Joint Research / Invited
• [Presentation] Live imaging of microtubule dynamics during plant zygote polarization (2017)
  • Author(s): 植田美那子
  • Organizer: 第69回日本細胞生物学会大会
  • Invited
• [Presentation] Live-cell imaging of the intracellular dynamics underlying the zygote polarization in Arabidopsis (2016)
  • Author(s): Yusuke Kimata, Daisuke Kurihara, Takumi Higaki, Tomomi Yamada, Seiichiro Hasezawa, Tetsuya Higashiyama and Minako Ueda
  • Organizer: 27th International Conference on Arabidopsis Research (ICAR2016)
  • Place of Presentation: 慶州 (韓国)
  • Year and Date: 2016-06-29
  • Int'l Joint Research / Invited
• [Remarks] 植物では両親の遺伝子の協力によって子供の形ができる
  • URL: http://www.itbm.nagoya-u.ac.jp/ja/research/2017/04/GandD-Parents.php