Novel methods for live-imaging and chemical screening to reveal the molecular mechanism of plant asymmetric cell division
| Project/Area Number |
16K14753
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
Ueda Minako 名古屋大学, 理学研究科(WPI), 特任講師 (20598726)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 植物受精卵 / ライブイメージング / 化合物スクリーニング / 植物 / 発生 / 細胞極性 / イメージング |
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| Outline of Final Research Achievements |
In most flowering plants, the asymmetric cell division of the zygote is the initial step in establishing the apical-basal axis. In Arabidopsis thaliana, the zygote is polarized, possessing the nucleus at the apical tip in the elongated cell. In spite of its obvious asymmetry, the real-time dynamics of the zygote polarization steps was poorly understood. Therefore we established a live-cell imaging system to visualize intracellular dynamics of the zygote through the ovule (Ueda and Berger, 2018). By combining this system with various specific inhibitors and mutants, we assessed the dynamics and roles of vacuoles in the zygote polarization (Kimata et. al., 2019). We also have found novel compounds, which effectively inhibit the zygotic division (in manuscript preparation).
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| Academic Significance and Societal Importance of the Research Achievements |
研究代表者らは、世界で初めて、植物の受精卵が極性化する動態をリアルタイムで観察することに成功した。さらに、これまで単なる水袋だと考えられてきた液胞のダイナミックな動きが、受精卵の非対称分裂だけでなく、その後の形作りにも必須であることも突き止めた。ほとんどの植物において、受精卵は大きな液胞を持つことから、本研究が明らかにした仕組みは、植物に共通した普遍的な機構であると期待される。液胞は植物だけでなく、真菌類にまで保存された基本的な細胞内小器官のため、植物の形が複雑に進化してきた経緯の理解や、農業技術への応用など、さまざまな分野への展開が期待できる。
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote.2016
Author(s)
Kimata, Y., Higaki, T., Kawashima, T., Kurihara, D., Sato, Y., Yamada, T., Hasezawa, S., Berger, F., Higashiyama, T., and Ueda, M.
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 113
Issue: 49
Pages: 14157-14162
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Combination of synthetic chemistry and live-cell imaging identified a rapid cell division inhibitor in tobacco and Arabidopsis thaliana2016
Author(s)
Nambo, M., Kurihara, D., Yamada, T., Nishiwaki-Ohkawa, T., Kadofusa, N., Kimata, Y., Kuwata, K., Umeda, M. and Ueda, M.
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Journal Title
Plant Cell Physiol.
Volume: 57
Issue: 11
Pages: 2255-2268
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Live-cell imaging of the axis formation in Arabidopsis zygote2018
Author(s)
Kimata, Y., Ando, N., Kato, T., Higaki, T., Kurihara, D., Yamada, T., Segami, S., Morita, M. T., Maeshima, M., Hasezawa, S., Higashiyama, T., Tasaka, M., Ueda, M.
Organizer
The 25th International Congress on Sexual Plant Reproduction (25th ICSPR)
Related Report
Int'l Joint Research
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[Presentation] Live-cell imaging of the intracellular dynamics of Arabidopsis zygote2018
Author(s)
Kimata, Y., Ando, N., Higaki, T., Kurihara, D., Kato, T., Yamada, T., Segami, S., Morita, M. T., Maeshima, M., Hasezawa, S., Higashiyama, T., Tasaka, M., Ueda, M.
Organizer
GARNet2018
Related Report
Int'l Joint Research / Invited
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