Role of epidermis in twisting growth of axial organs

Research Project

Project/Area Number	16K14755
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Plant molecular biology/Plant physiology
Research Institution	Nara Institute of Science and Technology
Principal Investigator	Hashimoto Takashi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (80180826)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	ねじれ / 微小管 / 左右性 / シロイヌナズナ / 表皮細胞 / 根 / チューブリン / 植物ホルモン / 成長生理 / 全能性 / 細胞伸長
Outline of Final Research Achievements	In this study, microtubule-regulating factors and cell elongating-regulating factors were expressed cell-type specifically in Arabidopsis roots, and two models (the epidermis leading model and the growth compensatory model) on organ twisting with defined chilarity were tested. When several mutant tubulins were expressed specifically at root epidermis, root epidermis grew normally and overall root growth and morphology were almost normal. When katanin and SAUR9 were used to generate growth differences between cortex and epidermis, growth inhibition and slanting growth were marginally observed. These results do not support simply either one of the proposed models.