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Establishment of molecular genetic methods for plant parasitic nematodes.

Research Project

Project/Area Number 16K14757
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionKumamoto University

Principal Investigator

Sawa Shinichiro  熊本大学, 大学院先端科学研究部(理), 教授 (00315748)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords植物感染性センチュウ / 技術開発 / 植物感染性線虫 / M. incognita / ネコブセンチュウ
Outline of Final Research Achievements

In order to examine molecular mechanisms of plant parasitic nematodes on successful plant infection, we have tried to develop two kind of new technologies;
1 New plant parasitic nematode culture system: Previously, we could grow nematodes only using tomato or other crops (big plants), and it need space to culture. In order to grow nematode in a small space, we have developed new method to culture in vitro, sterilized plates. In the plates, plant root was artificially cultured, and M. incognita was infected to the sterilized root in petri dish. We have confirmed that the M. incognita successfully migrate into the root, induce gall formation, and egg mass formation, indicating that we could grow the nematode throughout their life cycle in petri dish. Now, we can culture and maintain many kind of nematodes in a small space.
2 New method to manipulate gene expression levels of plant parasitic nematodes: We established RNAi technology, and we could knock down RNA levels in nemtadoes.

Academic Significance and Societal Importance of the Research Achievements

今回、本研究では、植物感染性線虫の感染過程において、線虫側で働く遺伝子を同定するために必要な分子遺伝学的解析技術を確立すると共に、新しい線虫培養法の確立も目指した。これまで、トマト等の大型の作物を用いて培養していた植物感染性センチュウを、滅菌プレート内で培養し、ライフサイクルをまわすことが可能となった。このことは、小スペースで様々なセンチュウを培養可能にすることから、センチュウの様々な系統を維持することが可能となり、様々なセンチュウ研究に供することが可能となった。また、センチュウ遺伝子の解析のため、RNAi技術を開発した。このことから、遺伝子ノックアウトによる機能会関が可能となった。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (16 results)

All 2019 2018 2017

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 3 results,  Invited: 10 results)

  • [Journal Article] Regulation of Root-Knot Nematode Behavior by Seed-Coat Mucilage-Derived Attractants.2019

    • Author(s)
      Tsai, A, Y-L., Higaki, T., Nguyen, C-N., Perfus-Barbeoch, L., Favery, B., and Sawa, S.
    • Journal Title

      Mol. Plants.

      Volume: 12 Issue: 1 Pages: 99-122

    • DOI

      10.1016/j.molp.2018.11.008

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Control of haploid organ size by CLE peptide signaling in Marchantia polymorpha. PLOS Genet.2019

    • Author(s)
      Hirakawa, Y., Uchida, N., Yamaguchi, Y., L., Tabata, R., Ishida, S., Ishizaki, K., Nishihama, R., Kohchi, T., Sawa, S#. and Bowman, J. L.
    • Journal Title

      In Press #Corresponding author.

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Connecting cells with metro: diverse function of peptide hormones in plant development.2019

    • Author(s)
      Hirakawa, Y., and Sawa, S.
    • Journal Title

      Curr. Opin. Plant Biol.In Press.

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
  • [Journal Article] A collection of mutants for CLE-peptide-encoding genes in Arabidopsis generated by CRISPR/Cas9 mediated gene targeting2017

    • Author(s)
      Ishida, T., Yamaguchi, Y., Yoshimura, M., Imamura, Y., Shimaoka, C., and Sawa, S.
    • Journal Title

      Plant Cell Physiol.

      Volume: 58 Pages: 1848-1856

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Root-knot and cyst nematodes activate procambium-associated genes in Arabidopsis roots.2017

    • Author(s)
      Yamaguchi, Y., Suzuki, R., Cabrera J., Nakagami, S., Sagara, T., Ejima C., Sano, R., Aoki, Y., Olmo, R., Kurata, T., Obayashi T., Demura, T., Ishida, T., Escobar, C., and Sawa, S
    • Journal Title

      Frontiers in Plant Science

      Volume: 8 Pages: 1195-1195

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] ネコブセンチュウ感染抵抗性トマトの原因究明と応用2019

    • Author(s)
      澤 進一郎
    • Organizer
      くまもと産業復興支援プロ ジェクトフォーラム,
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 踊る農業大捜査線 ―害虫被害を封鎖せよー2018

    • Author(s)
      澤 進一郎
    • Organizer
      くまだい研究フェア2018
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] キヅナのつむぎ方から生き物の生き様を学ぼう,2018

    • Author(s)
      澤 進一郎
    • Organizer
      2018 年度遺伝学研究所研究会「イネ分子遺伝学の飛躍」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 植物規制性線虫- 植物間コミュニケーションの実態解明,2018

    • Author(s)
      澤 進一郎
    • Organizer
      第22 回天然薬物の開発と 応用シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 植物構造システム形成における内生・外生プログラムによる力学的最適化,2018

    • Author(s)
      澤 進一郎
    • Organizer
      植物の 力学的最適化戦略に基づくサステナブル構造システムの基盤創成キックオフシンポジ ウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] サツマイモネコブセンチュウの誘引物質の解析2018

    • Author(s)
      澤 進一郎
    • Organizer
      日本線虫学会大会公開シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 植物寄生性線虫ー植物館コミュニケーションの実体解明2018

    • Author(s)
      澤 進一郎
    • Organizer
      科学コミュニケーション のフロンティア第3 回 公開シンポジウム,
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物感染性線虫の感染過程における細胞壁成分の機能と役割2017

    • Author(s)
      大田守浩,Allen Yi-Lun Tsai,鈴木れいら,島岡知恵,石田喬志,澤進一郎
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 植物感染性線虫の植物感染に関わるホルモンの役割2017

    • Author(s)
      澤 進一郎
    • Organizer
      ConBio2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanisms of gall development during plant parasitic nematode, M. incognita infection2017

    • Author(s)
      澤 進一郎
    • Organizer
      GDRI meeting 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Release of novel root-knot nematode attractants through seed coat mucilage extrusion2017

    • Author(s)
      澤 進一郎
    • Organizer
      International workshop on Plant Parasitic Nematode in Kumamoto
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2016-04-21   Modified: 2020-03-30  

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