Research Project
Grant-in-Aid for Challenging Exploratory Research
In order to examine molecular mechanisms of plant parasitic nematodes on successful plant infection, we have tried to develop two kind of new technologies;1 New plant parasitic nematode culture system: Previously, we could grow nematodes only using tomato or other crops (big plants), and it need space to culture. In order to grow nematode in a small space, we have developed new method to culture in vitro, sterilized plates. In the plates, plant root was artificially cultured, and M. incognita was infected to the sterilized root in petri dish. We have confirmed that the M. incognita successfully migrate into the root, induce gall formation, and egg mass formation, indicating that we could grow the nematode throughout their life cycle in petri dish. Now, we can culture and maintain many kind of nematodes in a small space.2 New method to manipulate gene expression levels of plant parasitic nematodes: We established RNAi technology, and we could knock down RNA levels in nemtadoes.
今回、本研究では、植物感染性線虫の感染過程において、線虫側で働く遺伝子を同定するために必要な分子遺伝学的解析技術を確立すると共に、新しい線虫培養法の確立も目指した。これまで、トマト等の大型の作物を用いて培養していた植物感染性センチュウを、滅菌プレート内で培養し、ライフサイクルをまわすことが可能となった。このことは、小スペースで様々なセンチュウを培養可能にすることから、センチュウの様々な系統を維持することが可能となり、様々なセンチュウ研究に供することが可能となった。また、センチュウ遺伝子の解析のため、RNAi技術を開発した。このことから、遺伝子ノックアウトによる機能会関が可能となった。
All 2019 2018 2017
All Journal Article (5 results) (of which Int'l Joint Research: 1 results, Peer Reviewed: 3 results, Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 3 results, Invited: 10 results)
Mol. Plants.
Volume: 12 Issue: 1 Pages: 99-122
10.1016/j.molp.2018.11.008
In Press #Corresponding author.
Volume: 印刷中
Curr. Opin. Plant Biol.In Press.
Plant Cell Physiol.
Volume: 58 Pages: 1848-1856
Frontiers in Plant Science
Volume: 8 Pages: 1195-1195
