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Molecular genetic studies to overcome continuous light injury in plants.

Research Project

Project/Area Number 16K14763
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research Institution岡山県農林水産総合センター生物科学研究所

Principal Investigator

Goto Koji  岡山県農林水産総合センター生物科学研究所, その他部局等, 専門研究員 (00251489)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords植物の連続光障害 / トマトゲノム / 概日リズム / 光障害 / オミクス解析 / 植物 / 遺伝学 / 生理学
Outline of Final Research Achievements

We discovered a tomato cultivar that shows tolerance to continuous light injury, although the usual tomato shows physiological disorder by continuous light irradiation for 24 hours a day. First, we examined the phenotypes of continuous light injury, and chose the indicator phenotypes those showing a large quantitative difference between tolerant and sensitive cultivars. Next, 20 tolerant individuals were selected using the indicator phenotype from the F2 generation obtained by crossing a tolerant cultivar with a sensitive one. This was used to map loci involved in continuous light tolerance by single nucleotide polymorphism detection using a next-generation sequencer. Based on mapping and sequence variation within the gene of tolerant and of sensitive cultivars, we narrowed down to 72 genes. In addition, the change of gene expression in normal LD-conditions and continuous light conditions was analyzed by the RNA-seq method.

Academic Significance and Societal Importance of the Research Achievements

食料やエネルギー源として植物や農作物の生産効率の向上は極めて重要な課題である。連続光障害耐性品種を用いて行った実験では、栄養成長期において、16時間明期8時間暗期の明暗条件と比べて、24時間明期の連続光条件では、2倍以上の新鮮重量が得られた。つまり、1.5倍の光照射時間の増加に対し、2倍の成長促進が得られたことになる。この様に、連続光栽培は投入するエネルギーコスト以上に、植物の成長を促進する栽培法である。
本研究によって得られた成果を利用して、交配育種によって連続光障害耐容性を持つ栽培トマト品種を作製すれば、人工光型植物工場等に適した品種として生産性の向上に活用できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016

All Presentation (11 results) (of which Int'l Joint Research: 5 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] Day-length dependent flowering time regulation in tomato.2019

    • Author(s)
      Chie Moriya and Koji Goto
    • Organizer
      The 60th Annual Meeting of the Japanese Society of Plant Physiologists
    • Related Report
      2018 Annual Research Report
  • [Presentation] 高温短日条件下の育苗がトマト一般品種の生育に及ぼす影響2019

    • Author(s)
      磯山陽介・谷本恵美・山田瑞樹・今西俊介・後藤弘爾・礒崎真英
    • Organizer
      園芸学会平成31年度春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Structural and functional analysis of tomato flowering genes belong to FT clade.2018

    • Author(s)
      Goto, K., and Moriya, C.
    • Organizer
      The 25th International Congress on Sexual Plant Reproduction.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of the genes that regulate tomato flowering by using tomato wild relatives and genome editing system.2018

    • Author(s)
      森谷智恵、後藤弘爾
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Functional analysis of tomato flowering genes of FT clade2018

    • Author(s)
      C. Moriya and K. Goto
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Functional analysis of Tomato florigen paralog SP6A.2017

    • Author(s)
      Chie Moriya, Mizuki Yamada, Koji Goto
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Goto, K., and Moriya, C.2017

    • Author(s)
      Functional analysis of tomato flowering genes.
    • Organizer
      Workshop on molecular Mechanisms Controlling Flower Development.
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional Analysis of Tomato Flowering Genes of FT-clade.2017

    • Author(s)
      C. Moriya and K. Goto
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of Tomato florigen paralog genes: To understand the flowering habit of tomato.2016

    • Author(s)
      C. Moriya, M. Yamada and K. Goto
    • Organizer
      CSHA Meeting: Latest Advances in Plant Development & Environmental Response
    • Place of Presentation
      Awaji Yumebutai Conference Center, Japan
    • Year and Date
      2016-11-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of Tomato florigen paralog SP6A2016

    • Author(s)
      C. Moriya, M. Yamada and K. Goto
    • Organizer
      The 13th Japan Solanaceae Consortium JSOL2016
    • Place of Presentation
      International Christian University, Mitaka, Japan
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Functional analyses of a tuberigen homologue in tomato.2016

    • Author(s)
      C. Moriya, M. Yamada and K. Goto
    • Organizer
      The 13th Solanaceae Conference SolGenomics: From Advances to Applications
    • Place of Presentation
      U.C. Davis. California USA
    • Year and Date
      2016-09-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] 花成が促進されたトマト植物及びその生産方法2019

    • Inventor(s)
      後藤弘爾、森谷智恵
    • Industrial Property Rights Holder
      後藤弘爾、森谷智恵
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-028255
    • Filing Date
      2019
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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