A new development of prolactin research to understanding the origin and multi-functionalization

• Project/Area Number: 16K14766
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: The University of Tokyo
• Principal Investigator: HYODO SUSUMU 東京大学, 大気海洋研究所, 准教授 (40222244)
• Co-Investigator(Kenkyū-buntansha): 岡田 令子 静岡大学, 理学部, 講師 (50386554) ; 鈴木 雅一 静岡大学, 理学部, 教授 (60280913)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: プロラクチン / 軟骨魚類 / 両生類 / 脳下垂体 / 環境適応 / 変態 / 進化 / 受容体 / 体液調節 / 多機能化

Outline of Final Research Achievements

The aim of this study is to understanding origin and evolution of pituitary hormone, prolactin (PRL). Genome and transcriptome analyses revealed that PRL and its specific receptor exist in elasmobranchs, suggesting that duplication and subsequent diversification of the growth hormone/PRL family already occurred when the cartilaginous fish lineage diverged. The cells expressing PRL receptor located close to the PRL-producing cells in the rostral pars distalis of pituitary of juvenile bull shark, implying that one of the original functions of PRL is paracrine control of pituitary function. Meanwhile, in the bullfrog, PRL1B, which is the orthologue of fish PRL, showed high expression in the pituitary of pre-metamorphic tadpoles, while tetrapod-type PRL1A, which is duplicated from the PRL1B gene, was highly expressed in the metamorphic tadpole and adult pituitaries. These achievements offer new insights into molecular and functional evolution of multifunctional PRL in vertebrates.

Research Products

• [Journal Article] A possible principal function of corticosteroid signaling that is conserved in vertebrate evolution: Lessons from receptor-knockout small fish (2018)
  • Author(s): Sakamoto Tatsuya、Hyodo Susumu、Takagi Wataru
  • Journal Title: Journal of Steroid Biochemistry and Molecular Biology Volume: - Pages: 57-61
  • DOI: 10.1016/j.jsbmb.2018.02.011
  • Peer Reviewed / Open Access
• [Journal Article] Distributional shift of urea production site from the extraembryonic yolk sac membrane to the embryonic liver during the development of cloudy catshark ( Scyliorhinus torazame ) (2017)
  • Author(s): Takagi Wataru、Kajimura Makiko、Tanaka Hironori、Hasegawa Kumi、Ogawa Shuntaro、Hyodo Susumu
  • Journal Title: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology Volume: 211 Pages: 7-16
  • DOI: 10.1016/j.cbpa.2017.05.019
  • Peer Reviewed
• [Journal Article] Migratory appendicular muscles precursor cells in the common ancestor to all vertebrates. (2017)
  • Author(s): Eri Okamoto, Rie Kusakabe, Shigehiro Kuraku, Susumu Hyodo, Alexandre Robert-Moreno, Koh Onimaru, James Sharpe, Shigeru Kuratani and Mikiko Tanaka
  • Journal Title: Nat. Ecol. Evol. Volume: 1 Issue: 11 Pages: 1731-1736
  • DOI: 10.1038/s41559-017-0330-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary (2017)
  • Author(s): Imamichi, Y., Sekiguchi, T., Kitano, T., Kajitani, T., Okada, R., Inaoka, Y., Miyamoto, K., Uwada, J., Takahashi, S., Nemoto, T., Mano, A., Khan, M.R.I., Islam, M.T., Yuhki, K., Kashiwagi, H., Ushikubi, F., Suzuki, N., Taniguchi, T., Yazawa, T.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 8374-8374
  • DOI: 10.1038/s41598-017-08780-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sulfate transporters involved in sulfate secretion in the kidney are localized in the renal proximal tubule II of the elephant fish (Callorhinchus milii). (2016)
  • Author(s): Hasegawa, K., Kato, A., Watanabe, T., Takagi, W., Romero, M., Bell, J.D., Toop, T., Donald, J.A. and Hyodo, S
  • Journal Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 311 Issue: 1 Pages: 66-78
  • DOI: 10.1152/ajpregu.00477.2015
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Distribution and dynamics of branchial ionocytes in houndshark reared in full-strength and diluted seawater environments (2016)
  • Author(s): Souichirou Takabe, Mayu Inokuchi, Yoko Yamaguchi and Susumu Hyodo
  • Journal Title: Comparative Biochemistry and Physiology, PartA Volume: 198 Pages: 22-32
  • DOI: 10.1016/j.cbpa.2016.03.019
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Atmospheric depression-mediated water temperature changes affect the vertical movement of chum salmon Oncorhynchus keta (2016)
  • Author(s): Takashi Kitagawa, Susumu Hyodo, Katsufumi Sato
  • Journal Title: Marine Environmental Research Volume: 119 Pages: 72-78
  • DOI: 10.1016/j.marenvres.2016.05.016
  • Peer Reviewed / Open Access
• [Journal Article] On the vagal cardiac nerves, with special reference to the early evolution of the head-trunk-interface. (2016)
  • Author(s): Higashiyama, H., Hirasawa, T., Oisi, Y., Sugahara, F., Hyodo, S., Kanai, Y., and Kuratani, S.
  • Journal Title: J. Morphol. Volume: 277 Issue: 9 Pages: 1146-1158
  • DOI: 10.1002/jmor.20563
  • Peer Reviewed
• [Journal Article] Arginine vasotocin is the major adrenocorticotropic hormone-releasing factor in the bullfrog Rana catesbeiana. (2016)
  • Author(s): Okada. R., Yamamoto, K., Hasunuma, I., Asahina, J., Kikuyama, S.
  • Journal Title: General and Comparative Endocrinology Volume: 237 Pages: 121-130
  • DOI: 10.1016/j.ygcen.2016.08.014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 両生類の変態：分子から個体レベルの制御 (2016)
  • Author(s): 岡田令子、鈴木賢一
  • Journal Title: 生物科学（日本生物科学者協会編集） Volume: 67 Pages: 146-153
  • Peer Reviewed
• [Presentation] 広塩性軟骨魚オオメジロザメの河川内生息環境と体液調節 (2017)
  • Author(s): 今関到、櫻井もも子、立原一憲、兵藤晋
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学，東京都港区
  • Year and Date: 2017-03-26
• [Presentation] サケの母川回帰行動と性成熟との関係に関する研究 (2017)
  • Author(s): 野畑重教、青木良徳、伯耆匠二、北川貴士、佐藤克文、竹井祥郎、兵藤晋
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学，東京都港区
  • Year and Date: 2017-03-26
• [Presentation] 岩手県大槌湾に回帰するシロサケ親魚のモニタリング (2017)
  • Author(s): 日下部誠、野畑重教、斉藤楓、池羽希理子、今関到、竹井祥郎、兵藤晋
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学，東京都港区
  • Year and Date: 2017-03-26
• [Presentation] Molecular and functional evolution of neurohypophysial hormone system: with special reference to a possible function of newly discovered V2b receptor in catshark (2017)
  • Author(s): Susumu Hyodo
  • Organizer: 18th International Congress of Comparative Endocrinology
  • Int'l Joint Research / Invited
• [Presentation] Physiological and ecological investigation of euryhaline bull shark using captive and wild individual (2017)
  • Author(s): Susumu Hyodo
  • Organizer: University of Hawaii and University of Tokyo Joint Symposium on Ocean, Coastal, and Atmospheric Sciences
  • Int'l Joint Research / Invited
• [Presentation] ネフロンの比較形態と腎機能：サメのネフロンを中心に (2017)
  • Author(s): 兵藤晋
  • Organizer: Tokyo Vertebrate Morphology Meeting
  • Invited
• [Presentation] 魚類の体液調節 (2017)
  • Author(s): 兵藤晋
  • Organizer: 第35回内分泌代謝学サマーセミナー
  • Invited
• [Presentation] 海洋生物研究への招待：サメ類をモデルに (2017)
  • Author(s): 兵藤晋
  • Organizer: 第57回生命科学夏の学校
  • Invited
• [Presentation] トラザメ胚発生における消化吸収機構の発達 (2017)
  • Author(s): 本田祐基、小川展弘、柴垣和弘、田中宏典、兵藤晋
  • Organizer: 第88回日本動物学会大会
• [Presentation] トラザメ胚の腎発生とネフロン形成の機能形態学的解析 (2017)
  • Author(s): 小川駿太郎、田中宏典、柴垣和弘、兵藤晋
  • Organizer: 第88回日本動物学会大会
• [Presentation] ゲノムからみる脊椎動物内分泌系の進化：大規模配列情報整備から分子探索まで (2017)
  • Author(s): 工樂樹洋、兵藤晋、山口和晃
  • Organizer: 第42回日本比較内分泌学会大会
• [Presentation] 板鰓類の全ゲノムシーケンスから明らかとなった有顎脊椎動物ペプチドホルモンの起源と進化 (2017)
  • Author(s): 山口和晃、兵藤晋、工樂樹洋
  • Organizer: 第42回日本比較内分泌学会大会
• [Presentation] 淡水エイはどのようにして淡水環境に適応しているのか (2017)
  • Author(s): 野本昌代、工樂樹洋、西村理、種子島千春、M.K.S. Wong、渡邊太朗、兵藤晋
  • Organizer: 第3回サメ類研究座談会
• [Presentation] 広塩性オオメジロザメの生理生態学的研究 (2017)
  • Author(s): 兵藤晋
  • Organizer: 第3回サメ類研究座談会
• [Presentation] 両生類の水環境適応におけるアクアポリンの役割 (2017)
  • Author(s): 鈴木雅一，岡田令子
  • Organizer: 第88回日本動物学会大会
  • Invited
• [Presentation] 変態期のウシガエル幼生下垂体中の2種類のプロラクチンmRNAの発現様式 (2017)
  • Author(s): 伊藤希実、岡田令子、鈴木雅一、兵藤晋
  • Organizer: 平成29年度日本動物学会中部支部大会
• [Presentation] ニホンアマガエル凍結耐性におけるグルコースの役割 (2017)
  • Author(s): 岡田令子，阿達駿，岩﨑良平
  • Organizer: 第88回日本動物学会大会
• [Presentation] クサフグ間脳におけるウルトラディアン発現遺伝子の探索 (2016)
  • Author(s): 山田佑紀、渡邊太朗、土井啓行、兵藤晋、安東宏徳
  • Organizer: 第41回日本比較内分泌学会
  • Place of Presentation: 北里大学相模原キャンパス、神奈川県相模原市
  • Year and Date: 2016-12-09
• [Presentation] サケ回帰親魚の性成熟が河川遡上行動に与える影響 (2016)
  • Author(s): 野畑重教、北川貴士、青木良徳、伯耆匠二、佐藤克文、竹井祥郎、兵藤晋
  • Organizer: 第41回日本比較内分泌学会
  • Place of Presentation: 北里大学相模原キャンパス、神奈川県相模原市
  • Year and Date: 2016-12-09
• [Presentation] ストレス刺激がサメの摂食に与える影響 (2016)
  • Author(s): 位寄あゆこ、Anderson WG.、兵藤晋
  • Organizer: 第41回日本比較内分泌学会
  • Place of Presentation: 北里大学相模原キャンパス、神奈川県相模原市
  • Year and Date: 2016-12-09
• [Presentation] トラザメの脳下垂体ならびに生殖器官における神経葉ホルモンの機能 (2016)
  • Author(s): 井上夏紀、山口陽子、兵藤晋
  • Organizer: 第41回日本比較内分泌学会
  • Place of Presentation: 北里大学相模原キャンパス、神奈川県相模原市
  • Year and Date: 2016-12-09
• [Presentation] オオメジロザメはなぜ川に上がるのか-西表島浦内川における遡上と生態 (2016)
  • Author(s): 櫻井もも子、今関到、兵藤晋、立原一憲
  • Organizer: 板鰓類シンポジウム
  • Place of Presentation: 葛西臨海水族園、東京都江戸川区
  • Year and Date: 2016-12-03
• [Presentation] オオメジロザメはなぜ川を上がることができるのか-飼育実験とトランスクリプトーム解析による広塩性メカニズムの研究 (2016)
  • Author(s): 今関到、若林翠、工樂樹洋、原雄一郎、櫻井もも子、立原一憲、兵藤晋
  • Organizer: 板鰓類シンポジウム
  • Place of Presentation: 葛西臨海水族園、東京都江戸川区
  • Year and Date: 2016-12-03
• [Presentation] Environmental adaptation of marine organisms (2016)
  • Author(s): Susumu Hyodo
  • Organizer: NTU-UTOKYO Joint Conference
  • Place of Presentation: 国立台湾大学、台北、台湾
  • Year and Date: 2016-11-30
  • Int'l Joint Research / Invited
• [Presentation] A new perspective for the origin of appendicular muscles (2016)
  • Author(s): Okamoto E., Kusakabe R., Kuraku S., Hyodo S., Onimaru K., Kuratani S., Tanaka M.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: Okinawa Institute for Science and Technology、沖縄県国頭郡恩納村
  • Year and Date: 2016-11-14
  • Int'l Joint Research / Invited
• [Presentation] Molecular evolution of prolactin: new insights from a recent study in cartilaginous fish (2016)
  • Author(s): Yamaguchi Y., Takage W., Kuraku S., Moriyama S., Bell JD., Seale AP., Lerner DT., Grau EG., Hyodo S.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: 沖縄コンベンションセンター、沖縄県宜野湾市
  • Year and Date: 2016-11-14
  • Int'l Joint Research / Invited
• [Presentation] The role of vasotocin V2a-type receptor in teleost: insights from studies using V2aR-knockout Medaka (2016)
  • Author(s): Konno N., Kanda S., Hyodo S., Matsuda K.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: 沖縄コンベンションセンター、沖縄県宜野湾市
  • Year and Date: 2016-11-14
  • Int'l Joint Research / Invited
• [Presentation] The origin of appendicular muscles: perspectives from the catshark (2016)
  • Author(s): Okamoto E., Kusakabe R., Kuraku S., Hyodo S., Onimaru K., Kuratani S., Tanaka M.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: 沖縄コンベンションセンター、沖縄県宜野湾市
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Electrophysiological and genetic demonstration that VT neuron is critical for water excretion in hypoosmotic conditions in medaka (2016)
  • Author(s): Shinya M., Kanda S., Takahashi A., Konno N., Hyodo S., Kagawa N., Oka Y.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: 沖縄コンベンションセンター、沖縄県宜野湾市
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Identification of a novel epithelial factor enhancing salinity tolerance in Japanese medaka (2016)
  • Author(s): Miyanishi H., Watanabe T., Hyodo S., Kaneko T.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: 沖縄コンベンションセンター、沖縄県宜野湾市
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Arginine vasotocin, but not authentic corticotropin-releasing factor, acts as the major factor for inducing the corticotropin release from the bullfrog pituitary. (2016)
  • Author(s): Okada, R., Yamamoto, K., Hasunuma, I., Asahina, J., Kikuyama, S.
  • Organizer: The 22nd International Congress of Zoology
  • Place of Presentation: 沖縄コンベンションセンター、沖縄県宜野湾市
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] ニホンアマガエルの凍結耐性に関わるグルコース輸送体（GLUT）の機能 (2016)
  • Author(s): 阿達駿，滝谷優，廣田敦司，鈴木雅一，岡田令子
  • Organizer: 日本動物学会2016中部支部大会
  • Place of Presentation: 静岡大学、静岡県静岡市
  • Year and Date: 2016-09-10
• [Presentation] 変態に関わる下垂体ホルモンの放出制御因子 (2016)
  • Author(s): 岡田令子
  • Organizer: 第2回次世代両生類研究会
  • Place of Presentation: 岡崎コンファレンスセンター、愛知県岡崎市
  • Year and Date: 2016-08-08
  • Invited
• [Presentation] New insights into the molecular evolution of classical hormones: lessons from cartilaginous fish (2016)
  • Author(s): Susumu Hyodo
  • Organizer: The 8th Congress of Asia and Oceania Society for Comparative Endocrinology
  • Place of Presentation: Korea University College of Medicine、ソウル、韓国
  • Year and Date: 2016-06-20
  • Int'l Joint Research / Invited
• [Book] Handbook of Hormones: Comparative Endocrinology for Basic and Clinical Research (2016)
  • Author(s): Susumu Hyodo
  • Publisher: Academic Press
• [Book] ホメオスタシスと適応（ホルモンから見た生命現象と進化シリーズ5） (2016)
  • Author(s): 兵藤 晋
  • Publisher: 裳華房
• [Remarks] 東京大学大気海洋研究所生理学分野
  • URL: http://physiol.aori.u-tokyo.ac.jp/seiri/
• [Remarks] 東京大学大気海洋研究所・生理学分野・兵藤晋
  • URL: http://seiri-aori.org/index.php?id=12