• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

3D morphometry of synapses with focused ion beam-scanning electron microscopy

Research Project

Project/Area Number 16K14767
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionNagoya University

Principal Investigator

KINOSHITA MAKOTO  名古屋大学, 理学研究科, 教授 (30273460)

Research Collaborator 深澤 有吾  福井大学, 教授
上田 奈津実 (石原 奈津実)  名古屋大学, 講師
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsシナプス / 形態計測 / ノックアウトマウス / 電子顕微鏡 / ssTEM / FIB-SEM / 3D再構築 / 3次元再構築 / 微細形態計測 / グリア突起 / 透過型電顕画像3次元再構築 / 集束イオンビーム走査電顕
Outline of Final Research Achievements

In this study we acquired serial 2D images from conventionally prepared tissue samples from wild type and genetically engineered mouse brain and compared 3D reconstructed images by using conventional ssTEM and FIB-SEM (in collaboration with the Fukazawa lab at Fukui Univ.). FIB-SEM, but not ssTEM, found a unique anomaly in synapse morphology, mainly due to the thickness along Z-axis (5nm vs. 50nm), whereas ssTEM could cover wider volume than FIB-SEM. Comparison of the two methods with immunogold-labeled samples is under investigation.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] CDC42EP4, a perisynaptic scaffold protein in Bergmann glia, is required for glutamatergic tripartite synapse configuration.2018

    • Author(s)
      Ageta-Ishihara N, Konno K, Yamazaki M, Abe M, Sakimura K, Watanabe M, Kinoshita M.
    • Journal Title

      Neurochem Int

      Volume: S0197-0186 Issue: 17 Pages: 30442-4

    • DOI

      10.1016/j.neuint.2018.01.003

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methods for immunoblot detection and electron microscopic localization of septin subunits in mammalian nervous systems.2016

    • Author(s)
      Parajuli LK, Ageta-Ishihara N, Ageta H, Fukazawa Y, Kinoshita M.
    • Journal Title

      Methods in Cell Biology

      Volume: 136 Pages: 285-294

    • DOI

      10.1016/bs.mcb.2016.04.021

    • ISBN
      9780128039984
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A FIB-SEM-based Method for High-Resolution Measurement of Morphological Parameters of Axospinous Synapses.2017

    • Author(s)
      Elhanbaly E, Ishikawa T, Ageta-Ishihara N, Kinoshita M, Fukazawa Y
    • Organizer
      The 1st ABiS Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A septin-mediated synaptic regulation required for spatial discrimination.2017

    • Author(s)
      Ageta-Ishihara N, Fukazawa Y, Takao K, Miyakawa T, Bito H, Inokuchi K, Kinoshita M.
    • Organizer
      Gordon Research Conference “Excitatory Synapses & Brain Function”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Unexpected motor and histopathological phenotype found in cerebellum-selective Sept7 knockout mice.2017

    • Author(s)
      Kinoshita M
    • Organizer
      The 8th Brain Research Institute International Symposium“Innovative progress of neuroscientific research through the use of advanced animal models”
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Book] DOJIN BIOSCIENCE SERIES 脳神経化学 19章: 神経ネットワークの形と機能を制御する細胞骨格系分子群2018

    • Author(s)
      木下 専(森泰生、尾藤晴彦編)
    • Total Pages
      368
    • Publisher
      化学同人
    • ISBN
      9784759817263
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi