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Deciphering the dynamics of chloroplast nucleoid network

Research Project

Project/Area Number 16K14768
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionKyoto University

Principal Investigator

Nishimura Yoshiki  京都大学, 理学研究科, 助教 (70444099)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords葉緑体DNA / 核様体 / マイクロ流体デバイス / ホリデイジャンクション解離酵素 / 葉緑体核様体 / ライブイメージング / 葉緑体ゲノム / 植物 / 遺伝学 / 進化
Outline of Final Research Achievements

Chloroplast (cp) DNA and the gene expression machinery are the fundamental system for the photosynthesis and biogenesis of chloroplasts. CpDNA molecules are packaged by multiple number of proteins into a minute globular structure called cp nucleoids. Cp nucleoids are the platform for multiple processes including DNA replication, repair, transcription, and inheritance. In this research, the process of chloroplast nucleoid division was visualized by our microfluidic device-based live imaging technology. Furthermore, chloroplast-localized Holliday junction resolvase was identified as a critical factor for the division of cp nucleoids.

Academic Significance and Societal Importance of the Research Achievements

遺伝情報の正確な伝達は、細胞分裂や有性生殖における最重要問題である。染色体の構造や形態、その遺伝様式はこれまで詳細かつ徹底した解析が行われてきたのに対し、葉緑体における染色体ともいうべき核様体の維持・遺伝機構は未だ謎に包まれている。本研究では、葉緑体核様体の分裂過程を生きた細胞で初めて捉えることに成功し、さらにその過程を制御する重要因子として、葉緑体DNAを正確に切り分けるハサミであるホリデイジャンクション解離酵素の遺伝子の同定に成功した。この酵素の遺伝子は緑色植物に広く保存されており、植物全般における葉緑体核様体の遺伝機構の一端が解明された。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (41 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (14 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 10 results,  Open Access: 9 results,  Acknowledgement Compliant: 3 results) Presentation (24 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Remarks (2 results)

  • [Int'l Joint Research] ブリティッシュコロンビア大学(カナダ)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Chloroplast nucleoids as a transformable network revealed by live imaging with a microfluidic device2018

    • Author(s)
      Kamimura Yoshitaka、Tanaka Hitomi、Kobayashi Yusuke、Shikanai Toshiharu、Nishimura Yoshiki
    • Journal Title

      Communications Biology

      Volume: 1:47 Issue: 1 Pages: 1-7

    • DOI

      10.1038/s42003-018-0055-1

    • NAID

      120006469191

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isolation and characterization of Chlamydomonas autophagy-related mutants in nutrient-deficient conditions.2018

    • Author(s)
      Kajikawa, M., Yamauchi, M., Shinkawa, H., Tanaka, M., Hatano, K., Nishimura, Y., Kato, M., Fukuzawa, H.
    • Journal Title

      Plant Cell Physiology

      Volume: 60 Issue: 1 Pages: 126-138

    • DOI

      10.1093/pcp/pcy193

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Holliday junction resolvases crucial for the chloroplast nucleoid morphology and segregation2018

    • Author(s)
      Kobayashi Yusuke、Misumi Osami、Nishimura Yoshiki
    • Journal Title

      PLANT MORPHOLOGY

      Volume: 30 Issue: 1 Pages: 73-81

    • DOI

      10.5685/plmorphol.30.73

    • NAID

      130007622405

    • ISSN
      0918-9726, 1884-4154
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 葉緑体DNAの分配を司るホリデイジャンクション解離酵素の発見2018

    • Author(s)
      西村芳樹
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 76(5) Pages: 404-404

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 葉緑体核様体の進化と構造のダイナミクス2018

    • Author(s)
      小林優介、三角修己、西村芳樹
    • Journal Title

      化学と生物

      Volume: 56(10) Pages: 651-658

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Journal Article] ゲノム編集の時代における「緑の酵母」クラミドモナスの復権2018

    • Author(s)
      浜地貴志・西村芳樹
    • Journal Title

      生物工学

      Volume: 96(8) Pages: 475-475

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Chloroplast nucleoids as a transformable network revealed by live-imaging with a microfluidic device2018

    • Author(s)
      Yoshitaka Kamimura, Hitomi Tanaka, Yusuke Kobayashi, Toshiharu Shikanai, Yoshiki Nishimura
    • Journal Title

      Communications Biology

      Volume: 印刷中

    • NAID

      120006469191

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The sexual reproduction program of Chlamydomonas reinhardtii.2018

    • Author(s)
      Yoshiki Nishimura
    • Journal Title

      MICROBIOLOGY MONOGRAPHS (Springer) Chlamydomonas Biology, Biotechnology and Biomedicine.

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Holliday junction resolvases mediate chloroplast nucleoid segregation2017

    • Author(s)
      Kobayashi Y, Misumi O, Odahara M, Ishibashi K, Hirono M, Hidaka K, Endo M, Sugiyama H, Iwasaki H, Kuroiwa T, Shikanai T, Nishimura Y.
    • Journal Title

      Science

      Volume: 356 Issue: 6338 Pages: 631-634

    • DOI

      10.1126/science.aan0038

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gene Regulatory Networks for the Haploid-to-Diploid Transition of Chlamydomonas reinhardtii2017

    • Author(s)
      Joo Sunjoo、Nishimura Yoshiki、Cronmiller Evan、Hong Ran Ha、Kariyawasam Thamali、Wang Ming Hsiu、Shao Nai Chun、El Akkad Saif-El-Din、Suzuki Takamasa、Higashiyama Tetsuya、Jin Eonseon、Lee Jae-Hyeok
    • Journal Title

      Plant Physiology

      Volume: 175 Issue: 1 Pages: 314-332

    • DOI

      10.1104/pp.17.00731

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 葉緑体ゲノムの分配はHollidayジャンクション解離酵素MOC1により保障される2017

    • Author(s)
      小林優介、三角修己、西村芳樹
    • Journal Title

      First Author's

      Volume: - Pages: 043

    • DOI

      10.7875/first.author.2017.043

    • Related Report
      2017 Research-status Report
  • [Journal Article] Dynamic interplay between nucleoid segregation and genome integrity in Chlamydomonas chloroplasts2016

    • Author(s)
      Odahara M, Kobayashi Y (equal contribution), Shikanai T, and Nishimura Y.
    • Journal Title

      Plant Physiology

      Volume: 172 Issue: 4 Pages: 2337-2346

    • DOI

      10.1104/pp.16.01533

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] C-Terminal Region of Sulfite Reductase Is Important to Localize to Chloroplast Nucleoids in Land Plants.2016

    • Author(s)
      Kobayashi, Y., Otani, T., Ishibashi, K., Shikanai, T., *Nishimura, Y.
    • Journal Title

      Genome Biol. Evol.

      Volume: 22 Issue: 5 Pages: 1459-1466

    • DOI

      10.1093/gbe/evw093

    • NAID

      120006822490

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Sequence of the Gonium pectorale Mating Locus Reveals a Complex and Dynamic History of Changes in Volvocine Algal Mating Haplotypes.2016

    • Author(s)
      Hamaji T, Mogi Y, Ferris PJ, Mori T, Miyagishima S, Kabeya Y, Nishimura Y, Toyoda A, Noguchi H, Fujiyama A, Olson BJ, Marriage TN, Nishii I, Umen JG, Nozaki H.
    • Journal Title

      G3

      Volume: 未定 Issue: 5 Pages: 1179-1189

    • DOI

      10.1534/g3.115.026229

    • NAID

      120005997691

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 葉緑体核様体のかたちを司るDNAリガーゼの同定2019

    • Author(s)
      西村芳樹、小林優介、浜地貴志、鹿内利治
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 核様体局在のHMGBタンパク質がDNA結合ドメインを2つもつ利点2019

    • Author(s)
      田草川真理, 小林優介, 深尾陽一朗, 日高久美, 遠藤政幸, 杉山弘, 宮川勇, 鹿内利治, 三角修己, 西村芳樹
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Chloroplast chromosomes on the move - Exploring the dynamics of chloroplast nucleoids -2018

    • Author(s)
      Yoshiki Nishimura
    • Organizer
      理研/ERATO/CSRSセミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 葉緑体核様体ネットワークの動態制御機構2018

    • Author(s)
      西村 芳樹,浜地 貴志,小林 優介,鹿内 利治
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Dynamic network structure of chloroplast nucleoids.2018

    • Author(s)
      Yoshiki Nishimura, Yusuke Kobayashi, Yoshitaka Kamimura, Osami Misumi, Toshiharu Shikanai
    • Organizer
      1st Asia-Oceania International Congress on Photosynthesis.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 葉緑体核様体のダイナミクスと進化2018

    • Author(s)
      西村芳樹
    • Organizer
      SIC研究部セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Dynamic transformable network of chloroplast nucleoids2018

    • Author(s)
      Yoshiki Nishimura, Yusuke Kobayashi, Yoshitaka Kamimura, Osami Misumi, Toshiharu Shikanai
    • Organizer
      International symposium on photosynthesis and chloroplast biogenesis.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamic Network Structure of Chloroplast Nucleoids.2018

    • Author(s)
      Yoshiki Nishimura, Yusuke Kobayashi, Yoshitaka Kamimura, Osami Misumi, Toshiharu Shikanai, Mari Takusagawa, Yusuke Kobayashi, Yoichiro Fukao, Isamu Miyakawa, Toshiharu Shikanai.
    • Organizer
      18th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ボルボックス系列異型配偶ユードリナにおける配偶子誘導要因の解析2018

    • Author(s)
      豊岡 博子,浜地 貴志,西村 芳樹,宮城島 進也,箕浦 高子,野崎 久義
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 母性遺伝メカニズムの解明に向けたクラミドモナス有性生殖のゲノム編集アプローチ2018

    • Author(s)
      浜地 貴志,山岡 尚平,鹿内 利治,西村 芳樹
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 雌雄異株種・同株種ボルボックスの性染色体領域・相同領域における比較ゲノム解析2018

    • Author(s)
      山本 荷葉子,浜地 貴志,豊岡 博子,新垣 陽子,野口 英樹,豊田 敦,水口 洋平,野崎 久義
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Biological analysis of plastid nucleoid-associated HMG-box protein in Chlamydomonas reinhardtii.2018

    • Author(s)
      Mari Takusagawa, Yusuke Kobayashi, Yoichiro Fukao, Isamu Miyakawa, Toshiharu Shikanai, Osami Misumi, and Yoshiki Nishimura.
    • Organizer
      International symposium on photosynthesis and chloroplast biogenesis.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Organelle nucleoid-associated HMG-box proteins may have been evolved independently through tandem domain duplications.2018

    • Author(s)
      Mari Takusagawa, Yusuke Kobayashi, Yoichiro Fukao, Isamu Miyakawa, Toshiharu Shikanai, Osami Misumi, and Yoshiki Nishimura
    • Organizer
      第14回植物縦横無尽の会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Organelle nucleoid-associated HMG-box proteins may have been evolved independently through tandem domain duplications.2018

    • Author(s)
      Mari Takusagawa, Yusuke Kobayashi, Yoichiro Fukao, Isamu Miyakawa, Toshiharu Shikanai, Osami Misumi, and Yoshiki Nishimura.
    • Organizer
      Gordon Research Conference, Mitochondria and Chloroplasts.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロ流体デバイスが捉えた葉緑体核様体の柔軟なネットワーク2018

    • Author(s)
      上村嘉誉、田中瞳、小林優介、鹿内利治、西村芳樹
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Identification of a Holliday junction resolvase in chloroplasts2017

    • Author(s)
      Kobayashi, Y., Misumi, O., Odahara, M., Hirono, M., Hidaka, K., Endo, M., Sugiyama, H., Kuroiwa, T., Shikanai, T., Nishimura, Y.
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Dynamics of chloroplast nucleoid divisions captured by microfluidic device2017

    • Author(s)
      Kamimura, Y., Kobayashi, Y., Shikanai, T., Nishimura, Y.
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Mechanism for the Cell-type Specific Expression of the Chloroplast ndh Genes in Zea Mays.2017

    • Author(s)
      Yano, H., Ishibashi, K., Nishimura, Y., Shikanai, T.
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Visualization of chloroplast-targeted autophagy in Chlamydomonas reinhardtii2017

    • Author(s)
      Li, N., Nishimura, Y., Ramundo, S., Hidema, J., Izumi, M.
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 葉緑体核様体の形や遺伝はホリデイジャンクション解離酵素によって制御される2017

    • Author(s)
      usuke Kobayashi, Osami Misumi, Masaki Odahara, Kota Ishibashi, Masafumi Hirono, Kumi Hidaka, Masayuki Endo, Hiroshi Sugiyama, Hiroshi Iwasaki, Tsuneyoshi Kuroiwa, Toshiharu Shikanai, Yoshiki Nishimura
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Dynamics of chloroplast nucleoid divisions captured by microfluidic device2017

    • Author(s)
      上村嘉誉, 小林優介, 鹿内利治, 西村芳樹
    • Organizer
      第58回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] オルガネラ核様体のダイナミズム2017

    • Author(s)
      西村芳樹
    • Organizer
      京都産業大学 バイオフォーラム
    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Invited
  • [Presentation] 亜硫酸還元酵素のC 末端領域は葉緑体核様体局在に重要である2016

    • Author(s)
      小林優介, 鹿内利治, 西村芳樹
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター、沖縄
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Restructuring of chloroplast nucleoids factor during the green plant evolution.2016

    • Author(s)
      Nishimura, Y.
    • Organizer
      The 13th International Colloquium of Endocytobiology and Symbiosis
    • Place of Presentation
      京都府立大学、京都
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 京都大学大学院 理学研究科 植物分子遺伝学研究室

    • URL

      http://www.bot.kyoto-u.ac.jp/j/5_iden.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 京都大学理学研究科植物教室 植物分子遺伝学

    • URL

      http://www.bot.kyoto-u.ac.jp/annual/5_iden.html

    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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