Functions of antisense RNAs associated with cathepsins in the gonadal follicles of the protochordate, Ciona intestinalis
| Project/Area Number |
16K14772
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Suntory Foundation for Life Sciences |
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Principal Investigator |
Sakai Tsubasa 公益財団法人サントリー生命科学財団, 生物有機科学研究所・統合生体分子機能研究部, 研究員 (40414122)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 進化 / RNA / 卵胞 / カタユウレイボヤ / antisense RNA / 初期卵胞 / カテプシン / follicle / ascidian / cathepsins / Cathepsin family / follicles / Ciona intestinalis / beads |
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| Outline of Final Research Achievements |
In RNA-seq analysis using sense RNA probe beads, we identified antisense (as)RNA showing a sequence complementary to the mRNA of Ci-ctsb, d, h and l from early stage follicles of the ascidian, Ciona intestinalis. We found 78 and 51-amino acid residue peptides predicted to be encoded in the asRNA of Ci-ctsb and h. The Immunostaining with specific antibodies showed that the translation products are expressed in test cells of the follicles of vitellogenic stage.
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| Academic Significance and Societal Importance of the Research Achievements |
RNAビーズとRNAseqを組み合わせた独創的方法でカタユウレイボヤ（Ciona intestinalis）のカテプシン(CTS)ファミリー遺伝子のアンチセンス(as)RNAを同定し、更にCi-ctsb、およびhの3’非翻訳領域と相補的配列を有するasRNAに翻訳領域を見出した。そして、特異的抗体によって卵黄形成期卵胞のtest細胞にこれらの発現が示唆され、asRNAの非コードとコーディングによる卵胞制御の可能性が示されたことは、asRNAの生物学的意義を考える上で極めて大きい。
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Report
(4 results)
Research Products
(9 results)
