Super-resolution live-imaging of RNA molecules
| Project/Area Number |
16K14773
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Masui Osamu 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 研究員 (30579305)
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| Research Collaborator |
KEPTEN Eldad Curie Institute
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | エピジェネティクス / non-coding RNA / X染色体不活性化 / Xist / 超解像顕微鏡 / ライブイメージング / Super-resolution / SIM / live-imaging / Xist RNA / Polycomb group / RNA / 遺伝子 / 核酸 / 生体分子 |
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| Outline of Final Research Achievements |
In this research project, we aimed to develop a new microscopic technology, super-resolution live-imaging, by combining 3D-SIM and time-lapse analysis. We have succeeded in establishing the basis of super-resolution live-imaging with 3D-SIM in this study. We have also published 2 scientific papers as relating researches. (1) We have developed a new methodology to visualize RNA molecules as GFP signals (Methods Mol Biol, in press). (2) We have clarified the spatial relationship between Xist RNA and PCGF3/5-PRC1 protein complex, a subcomplex of PRC1 (Science, 2017, 356, 1081-1084).
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation.2017
Author(s)
Almeida M, Pintacuda G, Masui O, Koseki Y, Gdula M, Cerase A, Brown D, Mould A, Innocent C, Nakayama M, Schermelleh L, Nesterova TB, Koseki H, Brockdorff N.
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Journal Title
Science
Volume: 356
Issue: 6342
Pages: 1081-1084
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes2017
Author(s)
Endoh M, Endo TA, Shinga J, Hayashi K, Farcas A, Ma KW, Ito S, Sharif J, Endoh T, Onaga N, Nakayama M, Ishikura T, Masui O, Kessler BM, Suda T, Ohara O, Okuda A, Klose RJ, Koseki H
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Journal Title
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes.2017
Author(s)
Endoh M, Endo TA, Shinga J, Hayashi K, Farcas A, Ma KW, Ito S, Sharif J, Endoh T, Onaga N, Nakayama M, Ishikura T, Masui O, Kessler BM, Suda T, Ohara O, Okuda A, Klose RJ, Koseki H.
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Journal Title
Elife
Volume: 6
Pages: 27970-27970
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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