| Project/Area Number |
16K14773
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Masui Osamu 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 研究員 (30579305)
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| Research Collaborator |
KEPTEN Eldad Curie Institute
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | エピジェネティクス / non-coding RNA / X染色体不活性化 / Xist / 超解像顕微鏡 / ライブイメージング / Super-resolution / SIM / live-imaging / Xist RNA / Polycomb group / RNA / 遺伝子 / 核酸 / 生体分子 |
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| Outline of Final Research Achievements |
In this research project, we aimed to develop a new microscopic technology, super-resolution live-imaging, by combining 3D-SIM and time-lapse analysis. We have succeeded in establishing the basis of super-resolution live-imaging with 3D-SIM in this study. We have also published 2 scientific papers as relating researches. (1) We have developed a new methodology to visualize RNA molecules as GFP signals (Methods Mol Biol, in press). (2) We have clarified the spatial relationship between Xist RNA and PCGF3/5-PRC1 protein complex, a subcomplex of PRC1 (Science, 2017, 356, 1081-1084).
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