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Analysis of learning systems in the insect microbrain by genome editing

Research Project

Project/Area Number 16K14774
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Animal physiology/Animal behavior
Research InstitutionHokkaido University

Principal Investigator

Mizunami Makoto  北海道大学, 理学研究院, 教授 (30174030)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords昆虫 / オクトパミン / ドーパニン / 連合学習 / 社会学習 / コオロギ / RNA干渉 / ゲノム編集 / オクトパミン受容体 / FoxP / ノックアウト / ドーパミン受容体 / 条件づけ / 学習 / 記憶 / ドーパミン / アデニル酸シクラーゼ / 神経行動学 / 神経科学 / ゲノム編集技術
Outline of Final Research Achievements

The purpose of this study was to establish techniques to knockout specific genes by using CRISPR/Cas9 methods in the cricket Gryllus bimaculatus to accelerate the study of molecular mechanisms of learning and memory. We found, at first, that knockout of Dop1 dopamine receptor gene resulted in impairment of social learning to learn aversive stimulus from the behavior of a conspecific but social learning to find rewarding stimulus by social learning was intact. The results suggest that Dop1 transmit reinforcement signals in aversive social learning. Next, we attempted to establish knockout cricket line with OA1 octopamine receptor but we were not yet successful. In addition, we performed RNAi studies to knockdown genes that code AC1 and AC2 adenylyl cyclase and FoxP transcription repressor. Unexpectedly, no defects in olfactory learning were found in AC1 or AC8 knockdown crickets. It was also unexpected that FoxP knockdown crickets exhibited impairments in long-term memory formation.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (21 results)

All 2017 2016 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results) Book (4 results) Remarks (1 results)

  • [Journal Article] Roles of dopamine neurons in mediating the prediction error in aversive learning in insects2017

    • Author(s)
      Terao Kanta、Mizunami Makoto
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 14694-14694

    • DOI

      10.1038/s41598-017-14473-y

    • NAID

      120006370603

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Group-housed females promote production of asexual ootheca in American cockroaches2017

    • Author(s)
      Katoh K., Iwasaki M., Hosono S., Yoritsue A., Ochiai M., Mizunami M., Nishino H.
    • Journal Title

      Zoological Letters

      Volume: 3 Issue: 1 Pages: 3-3

    • DOI

      10.1186/s40851-017-0063-x

    • NAID

      120005981462

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Complete identification of four giant interneuronssupplying mushroom body calyces in the cockroach Periplaneta americana2017

    • Author(s)
      Takahashi N., Katoh K., Watanabe H., Nakayama Y., Iwasaki M., Mizunami M., Nishino H.
    • Journal Title

      Journal of Comparative Neurology

      Volume: 525 Issue: 1 Pages: 204-230

    • DOI

      10.1002/cne.24108

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Roles of octopamine and dopamine neurons for mediating appetitive and aversive signals in Pavlovian conditioning in crickets2017

    • Author(s)
      M. Mizunami and Y. Matsumoto
    • Journal Title

      Frontier in Physiology

      Volume: 8 Pages: 1027-1027

    • DOI

      10.3389/fphys.2017.01027

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Searching for cognitive processes underlying insect learning2017

    • Author(s)
      Mizunami M, Matsumoto C-S, Matsumoto Y.
    • Journal Title

      Japanese Journal of Animal Psychology

      Volume: 67 Issue: 1 Pages: 1-10

    • DOI

      10.2502/janip.67.1.5

    • NAID

      130006894416

    • ISSN
      0916-8419, 1880-9022
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interaction of inhibitory and facilitatory effects of conditioning trials on long-term memory formation2016

    • Author(s)
      Hosono S, Matsumoto Y, Mizunami M.
    • Journal Title

      Learning & Memory

      Volume: 23 Issue: 12 Pages: 669-678

    • DOI

      10.1101/lm.043513.116

    • NAID

      120006366476

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Habit formation by extended Pavlovian training in crickets.2017

    • Author(s)
      Makoto Mizunami
    • Organizer
      Naito Conference on decision making in the brain.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] コオロギ古典的条件付けにおける習慣形成2017

    • Author(s)
      水波誠
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Contrasting social learning with live and dead conspecifics in cricket.2017

    • Author(s)
      Hiroki Ebina, Makoto Mizunami
    • Organizer
      日本比較生理生化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Roles of a transcription factor FoxP in olfactory learning in cricket2017

    • Author(s)
      Li Yaolong, Takayuki Watanabe, Makoto Mizunami
    • Organizer
      日本比較生理生化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コオロギの学習研究から何を学ぶか?2016

    • Author(s)
      水波誠
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-02
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Searching for cognitive processes involved in insect learning2016

    • Author(s)
      Makoto Mizunami.
    • Organizer
      日本動物心理学会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Different social learning from live and dead conspecifics in crickets.2016

    • Author(s)
      Hiroki Ebina, Makoto Mizunami
    • Organizer
      International Congress of Zoology
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The role of type I adenylyl cyclase AC1 in cricket learning.2016

    • Author(s)
      Satomi Katata, Takayuki Watanabe, Taro Mito, Makoto Mizunami,
    • Organizer
      International Congress of Zoology
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Toward elucidation of neural mechanisms underlying aversive learning in insects.2016

    • Author(s)
      Yoshitaka Hamanaka, Makoto Mizunami.
    • Organizer
      日本比較生理生化学会
    • Place of Presentation
      玉川大学(東京都町田市)
    • Year and Date
      2016-09-04
    • Related Report
      2016 Research-status Report
  • [Presentation] Studies on structure and function of insect microbrain.2016

    • Author(s)
      Makoto Mizunami.
    • Organizer
      日本比較生理生化学会
    • Place of Presentation
      玉川大学(東京都町田市)
    • Year and Date
      2016-09-03
    • Related Report
      2016 Research-status Report
    • Invited
  • [Book] 脳の神秘を探ってみよう2017

    • Author(s)
      水波誠
    • Total Pages
      10
    • Publisher
      朝日新聞出版
    • Related Report
      2017 Annual Research Report
  • [Book] The Cricket as a Model Organism2017

    • Author(s)
      Matsumoto Y and Mizunami M
    • Total Pages
      12
    • Publisher
      Springer, Tokyo.
    • Related Report
      2017 Annual Research Report
  • [Book] The Cricket as a Model Organism2017

    • Author(s)
      Mizunami M and Matsumoto Y
    • Total Pages
      12
    • Publisher
      Springer, Tokyo.
    • Related Report
      2017 Annual Research Report
  • [Book] Cambridge Univ Press2016

    • Author(s)
      Taya M, Vonkenburgh EV, Mizunami M, Nomura S.
    • Total Pages
      523
    • Publisher
      Bioinspired Actuators and Sensors
    • Related Report
      2016 Research-status Report
  • [Remarks] 水波誠研究室

    • URL

      https://www.sci.hokudai.ac.jp/~mizunami/MICROB~2/index.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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