| Project/Area Number |
16K14782
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松尾 拓哉 名古屋大学, 遺伝子実験施設, 助教 (00452201)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生物時計 / 概日時計 / TTFL / PTO / カサノリ |
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| Outline of Final Research Achievements |
Circadian clocks can oscillate without gene transcriptions in the cell nucleus but its mechanisms remain unknown. In this study, we tried to develop a luciferase-based monitoring system to monitor the oscillation in Acetabularia which is expected to contribute to the studies on the oscillatory system. Because of some difficulties in the development of luciferase reporter, we failed to detect significant luciferase signal from Acetabularia cells. However, on the other hand, we succeeded in monitoring of the oscillation by measuring the delayed light emission of chlorophylls. In addition, we found that the oscillation is sensitive to a phosphatase inhibitor, suggesting importance of phosphorylation in the oscillatory mechanism. This monitoring system will be useful for studies on the uncharacterized oscillatory mechanism in Acetabularia.
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