Regulation of somatic hypermutation on the immunoglobulin gene by novel functions of an RNA splicing factor
Project/Area Number |
16K14783
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Genetics/Chromosome dynamics
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Research Institution | Okayama University |
Principal Investigator |
KANAYAMA Naoki 岡山大学, 自然科学研究科, 准教授 (70304334)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | SRタンパク質 / スプライシング / AID / DT40 / 抗体 |
Outline of Final Research Achievements |
The affinity of antibodies is improved by somatic hypermutation on the immunoglobulin variable region gene. Although it has been shown that AID is essential for somatic hypermutation, it remains unclear how the AID-dependent mutation machinery works. In this study, functions of SRSF1-3, which is an isoform of the RNA splicing factor SRSF1 and essential for somatic hypermutation, were analyzed using a hypermutating B cell line. Results revealed that SRSF1-3 forms a protein complex with AID, and promotes somatic hypermutation through a mechanism linked to the C-terminal region of AID.
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Report
(3 results)
Research Products
(15 results)
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[Presentation] SRSF1-3 has a role in nuclear localization of AID by regulating its nuclear export2016
Author(s)
Kanayama, N., Kawaguchi, Y., Kawamoto, N., Nariki, H., Miyazaki, S., Yokoyama, K., Magari, M., Ohmori, H., Tokumitsu, H.
Organizer
International Congress of Immunology 2016
Place of Presentation
Melbourne, Australia
Year and Date
2016-08-21
Related Report
Int'l Joint Research
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[Presentation] Analysis of a role for SRSF1-3 in a transcription-coupled process during IgV hypermutation2016
Author(s)
Kawaguchi, Y., Yokoyama, K., Kawamoto, N., Nariki, H., Matsuyama, Y., Kanehiro, Y., Hikasa, T., Magari, M., Ohmori, H., Tokumitsu, H., Kanayama, N.
Organizer
International Congress of Immunology 2016
Place of Presentation
Melbourne, Australia
Year and Date
2016-08-21
Related Report
Int'l Joint Research
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