Evolution of membrane co-transport system- Is Na+ essential for co-transporter of marine algae ?
| Project/Area Number |
16K14786
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Kobe University |
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Principal Investigator |
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| Research Collaborator |
Ohnishi Miwa
Kawai Hiroshi
Isaka Wakana
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 共役輸送 / ナトリウムイオン / リン酸 / アオサ / シャジクモ / 膨圧調節 |
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| Outline of Final Research Achievements |
The co-transport system is known to be H+-coupling or Na+-coupling. The phosphate transport system of Chara braunii which is supposed to be the ancester of land-plants, is Na+ co-transport type. In order to clarify the evolution of this co-transport system, we aimed to analyze the molecular basis and evolution of the co-transport system by using the brackish green algae; Ulva compressa. We found that it can be grown even under Na+-deficient conditions, and the phosphate transport is both H+ type and Na+ type. Transcriptome analysis revealed the presence of two phosphate transporter genes supposed to be in the cell membrane. In further experiment, the Na+ dependency of the phosphate transporter gene of Chara braunii was confirmed using Xenopus unfertilized egg’s system.
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| Academic Significance and Societal Importance of the Research Achievements |
生体膜共役輸送系は、栄養の取り込みや老廃物の排出に働き、細胞存続の基盤となる輸送系である。H＋共役型とNa＋共役型が知られていて、植物と動物を区別する大きな要素である。陸上植物は、H＋共役型を持つことが知られている。本研究から陸上植物の祖先種であるシャジクモやアオサ類は、H＋型やNa＋型を持つことが明らかとなった。これら共役輸送系は、古くはH＋共役型から進化したと考えられているが、陸上植物の祖先種の植物群がNa＋型も持つことから、これらの共役輸送系がどのように進化してきたのかを、もう一度検討し直すことが必要となる。
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Molecular Components of Arabidopsis Intact Vacuoles Clarified with Metabolomic and Proteomic Analyses2018
Author(s)
Miwa Ohnishi, Aya Anegawa, Yuko Sugiyama, Kazuo Harada, Akira Oikawa, Yasumune Nakayama, Fumio Matsuda, Yukiko Nakamura, Ryosuke Sasaki, Chizuko Shichijo Patrick G. Hatcher, Hidehiro Fukaki, Shigehiko Kanaya, Koh Aoki, Mami Yamazaki, Eiichiro Fukusaki, Kazuki Saito, Tetsuro Mimura
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Journal Title
Plant and Cell Physiology
Volume: 69
Pages: 1353-1362
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Cadmium-induced changes in vacuolar aspects of Arabidopsis thaliana.2017
Author(s)
Shanti S. Sharma, Kotaro Yamamoto, Kohei Hamaji, Miwa Ohnishi, Aya Anegawa, Shashi Sharma, Sveta Thakur, Vijay Kumar, Tomohiro Uemura, Akihiko Nakano, Tetsuro Mimura
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Journal Title
Plant Physiol Biochem.
Volume: 114
Pages: 29-37
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Inositol hexakis phosphate is the seasonal phosphorus reservoir in the deciduous woody plant Populus alba L.2017
Author(s)
Kurita, Y., Baba, K., Ohnishi, M., Matsubara, R., Kosuge, K., Anegawa, A., Shichijo, C., Ishizaki, K., Kaneko, Y., Hayashi, M., Suzaki, T., Fukaki, H., Mimura, T.
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Journal Title
Plant Cell Physiol.
Volume: 58
Issue: 9
Pages: 1477-1485
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] A shortened annual cycle system; a tool for laboratory studies of seasonal phenomena in trees2017
Author(s)
Yuko Kurita, Satomi Kanno, Ryohei Sugita, Atsushi Hirose, Miwa Ohnishi, Ayumi Tezuka, Ayumi Deguchi, Keiko Kosuge, Kimitsune Ishizaki, Hidehiro Fukaki, Keitaro Tanoi, Tomoko M. Nakanishi, Kei’ichi Baba, Tetsuro Mimura, Atsushi J. Nagano
Organizer
Taiwan-Japan Plant Biology 2017
Related Report
Int'l Joint Research
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