Possible roles of the epitranstomic plasticity in establishing the host-symbiont relationship in the primary endosymbiosis

• Project/Area Number: 16K14788
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Matsuo Mitsuhiro 京都府立大学, 生命環境科学研究科, 特任助教 (70415298)
• Co-Investigator(Kenkyū-buntansha): 小保方 潤一 京都府立大学, 生命環境科学研究科, 教授 (50185667)
• Co-Investigator(Renkei-kenkyūsha): Murakami Akio 神戸大学, 内海域環境教育研究センター, 准教授 (50304134)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 一次細胞内共生進化 / エピトランスクリプトーム / RNAシュードウリジン化 / 有殻アメーバ / シアネレ / 細胞内共生進化 / 葉緑体 / RNA修飾

Outline of Final Research Achievements

Epitranscriptome means all the biochemical modifications of the RNA within a cell. Increasing evidence suggests that epitranscriptomic diversity makes a new layer of controlling the genomic functions in addition to the classical gene regulatory steps and epigenomic diversity. Our preliminary genome analysis of a photosynthetic amoeba, Paulinella micropora, suggests that the enzymes involved in RNA modification should be preferentially acquired by horizontally- and endosymbiotically-gene transfer at the early stage of the primary endosymbiotic evolution. To gain insight for the relationship between RNA modification and endosymbiotic evolution, we performed the comprehensive analysis of RNA pseudouridylation, one of major RNA modifications, in P. micropora. Our analysis detected the novel RNA pseudouridylations in the photosynthetic organelle of this organism, suggesting that the organelle epitranscriptome might be altered in the primary endosymbiotic evolution.

Research Products

• [Presentation] 真核光合成生物はどのように生まれたか？―光合成有殻アメーバのゲノム解析から見えてきた一次細胞内共生進化の初期プロセス (2017)
  • Author(s): 松尾充啓、潟端篤、水口洋平、野口英樹、豊田敦、藤山秋佐夫、鈴木穣、佐藤壮一郎、中山卓郎、神川龍馬、野村真未、稲垣祐司、石田健一郎、小保方潤一
  • Organizer: 第19回植物オルガネラワークショップ
  • Place of Presentation: 鹿児島大学、鹿児島
  • Year and Date: 2017-03-15
• [Presentation] 有殻アメーバのゲノム解読による一次細胞内共生進化の初期プロセスの解析 (2017)
  • Author(s): 松尾充啓、 潟端篤、立川誠、水口洋平、野口 英樹、豊田敦、藤山秋佐夫、鈴木穣、中山卓郎、神川龍馬、野村真未、稲垣祐司、石田健一郎、小保方 潤一
  • Organizer: 日本植物学会第81回大会
• [Presentation] Evolutionary roles of SL-trans-splicing in the primary endosymbiosis (2016)
  • Author(s): Mitsuhiro Matsuo, Atsushi Katahata, Soichirou Satoh, Motomichi Matsuzaki, Mami Nomura, Ken-ichiro Ishida, Yuji Inagaki and Junichi Obokata
  • Organizer: The 13th International Society of Endocytobiology and Symbiosis
  • Place of Presentation: Kyoto prefectural Univ. Kyoto, Japan
  • Year and Date: 2016-09-10
  • Int'l Joint Research