Regulation of circadian clock of the eukaryotic host cell by the chloroplast and its evolution
| Project/Area Number |
16K14791
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 細胞内共生 / 細胞内時計 / 光合成 / 概日リズム |
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| Outline of Final Research Achievements |
Chloroplasts were established through predation and temporary retention of photosynthetic prey and then as a facultative and permanent endosymbiotic relationship with photosynthetic organisms by a unicellular eukaryotic host cell. In this study, we investigated the mechanism regulating the host cell by the chloroplast and its evolution. We found that, in a unicellular red alga, photosynthetic activity in the chloroplast resets the circadian clock of the eukaryotic host cell through reducing power. In addition, we found that three species of amoebae feeding on photosynthetic prey are exposed to oxidative stress upon illumination. That stress decreased phagocytic uptake of prey whereas accelerated digestion of prey. These results suggest that there is a signal transduction mechanism by which reducing power generated by the chloroplast modulates eukaryotic host cell and that such mechanism has evolved from that in eukaryotes feeding on photosynthetic prey.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Development of a double nuclear gene-targeting method by two-step transformation based on a newly established chloramphenicol-selection system in the red alga Cyanidioschyzon merolae2017
Author(s)
Fujiwara, T., Ohnuma, M., Kuroiwa, T., Ohbayashi, R., Hirooka, S.and Miyagishima, S.
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Journal Title
Front. Plant Sci.
Volume: 8
Pages: 343-343
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Analyses of photosynthetic oxidative stress responses in gerbivolous unicellular organisms2016
Author(s)
Uzuka, A., Hirooka, S., Fujiwara, T., Kanesaki, Y., Yoshikawa, H., MIyagishima, S.
Organizer
Prostist-2016
Place of Presentation
Moscow, Russia
Year and Date
2016-06-06
Related Report
Int'l Joint Research
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