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Regulation of circadian clock of the eukaryotic host cell by the chloroplast and its evolution

Research Project

Project/Area Number 16K14791
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionNational Institute of Genetics

Principal Investigator

MIYAGISHIMA Shin-ya  国立遺伝学研究所, 細胞遺伝研究系, 教授 (00443036)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords細胞内共生 / 細胞内時計 / 光合成 / 概日リズム
Outline of Final Research Achievements

Chloroplasts were established through predation and temporary retention of photosynthetic prey and then as a facultative and permanent endosymbiotic relationship with photosynthetic organisms by a unicellular eukaryotic host cell. In this study, we investigated the mechanism regulating the host cell by the chloroplast and its evolution. We found that, in a unicellular red alga, photosynthetic activity in the chloroplast resets the circadian clock of the eukaryotic host cell through reducing power. In addition, we found that three species of amoebae feeding on photosynthetic prey are exposed to oxidative stress upon illumination. That stress decreased phagocytic uptake of prey whereas accelerated digestion of prey. These results suggest that there is a signal transduction mechanism by which reducing power generated by the chloroplast modulates eukaryotic host cell and that such mechanism has evolved from that in eukaryotes feeding on photosynthetic prey.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2017 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Development of a double nuclear gene-targeting method by two-step transformation based on a newly established chloramphenicol-selection system in the red alga Cyanidioschyzon merolae2017

    • Author(s)
      Fujiwara, T., Ohnuma, M., Kuroiwa, T., Ohbayashi, R., Hirooka, S.and Miyagishima, S.
    • Journal Title

      Front. Plant Sci.

      Volume: 8 Pages: 343-343

    • DOI

      10.3389/fpls.2017.00343

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 真核宿主細胞と細胞内共生体由来オルガネラの協調増殖機構2017

    • Author(s)
      宮城島 進也
    • Organizer
      第17回名古屋大学遺伝子実験施設公開セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 真核細胞と葉緑体・光合成性細胞内共生体の協調増殖機構2017

    • Author(s)
      宮城島 進也
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 真核藻類における細胞周期進行とエネルギー変換の時間分業2017

    • Author(s)
      宮城島 進也、恵良 厚子、廣岡 俊亮、藤原 崇之
    • Organizer
      日本植物学会・第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 真核宿主細胞と細胞内共生オルガネラの協調増殖機構2017

    • Author(s)
      宮城島 進也
    • Organizer
      日本進化学会シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Uses of acidic hot spring eukaryotic algae: pure and applied sciences2017

    • Author(s)
      宮城島進也
    • Organizer
      光合成科学:エネルギーとバイオマス
    • Place of Presentation
      東京工業大学
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 真核細胞と葉緑体の分裂同調化機構2017

    • Author(s)
      宮城島進也
    • Organizer
      真核細胞のオルガネラ研究最前線
    • Place of Presentation
      大阪大学
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Analyses of photosynthetic oxidative stress responses in gerbivolous unicellular organisms2016

    • Author(s)
      Uzuka, A., Hirooka, S., Fujiwara, T., Kanesaki, Y., Yoshikawa, H., MIyagishima, S.
    • Organizer
      Prostist-2016
    • Place of Presentation
      Moscow, Russia
    • Year and Date
      2016-06-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] Regulation of cell cycle progression by circadian rhythms in Cyanidioschyzon merolae. In Cyanidioschyzon merolae: A new model eukaryote for cell and organelle biology.2017

    • Author(s)
      Miyagishima, S.
    • Total Pages
      11
    • Publisher
      Springer
    • ISBN
      9789811061011
    • Related Report
      2017 Annual Research Report
  • [Remarks]

    • URL

      https://www.nig.ac.jp/nig/ja/research/organization-top/laboratories/miyagishima

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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