Development of microbial detection tool for link between phylogeny and function.

• Project/Area Number: 16K14799
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Kanazawa University
• Principal Investigator: Matsuura Norihisa 金沢大学, 環境デザイン学系, 助教 (90771585)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 16S rRNA遺伝子 / 機能遺伝子 / 遺伝子結合PCR / 未培養微生物 / 結合PCR

Outline of Final Research Achievements

We demonstrated the idea for link between 16S rRNA gene and functional gene. The two genes were combined from the microbial genome/cell by fusion PCR. However we found that PCR condition is little bit different with the microbial genome and the cell. Sequencing result showed that two genes were combined correctly.