| Project/Area Number |
16K14809
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
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| Research Institution | Gifu University |
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Principal Investigator |
Suzuki Tohru 岐阜大学, 応用生物科学部, 教授 (20235972)
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| Co-Investigator(Kenkyū-buntansha) |
岡本 朋子 岐阜大学, 応用生物科学部, 助教 (50588150)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | insect / yeast / flower / metagenome / chemical ecology / 共生 / 化学生態学 / メタゲノム解析 / 三者間の共生系 / ゲノム多形 / 昆虫 / 植物 / 微生物 / 進化 / ゲノム |
|---|
| Outline of Final Research Achievements |
The aromatic components produced by yeasts should play important roles in insects induction. We hypothesized that 'a tripartite symbiosis' has been established among flower - yeast - insects. In this study, we tried to verify this hypothesis from the standpoints of chemical symbiosis and metagenome analysis.
For yeast, rRNA D1 - D2 region, Saccharomyces cerevisiae, six microsatellites, Rubisco gene rbcL for flowers, insects for mitochondrial cytochrome C oxidase subunit I (COI) gene as the target, multiplex PCR Established the method. Simultaneous identification of yeasts, plants, insects was made possible from samples of 384 samples of plants at the same time.
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