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Ecophysiology of rare biosphere revealed by a combination of stable isotope probing and high-throughput sequencing

Research Project

Project/Area Number 16K14816
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Hori Tomoyuki  国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (20509533)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords遺伝子 / 生態学 / 微生物学 / 環境分析 / 環境技術 / 微生物
Outline of Final Research Achievements

In this study, high-sensitivity stable isotope probing was implemented to identify the hitherto unknown microorganisms, including rare biosphere, involved in the degradation of 1,4-dioxane (recalcitrant compound biodegradation) and the degradation of dead bacterial biomass (microbial food web) as the different metabolic functions of microbial communities in natural environments. The former research revealed that the co-existence and individually distinct dynamics of various 1,4-dioxane-degrading microorganisms played pivotal roles in the maintenance of the biological system removing the recalcitrant pollutant. The latter research demonstrated that a variety of bacteria were potentially competitive with pathogens due to their preferential assimilation of dead biomass in compost.

Academic Significance and Societal Importance of the Research Achievements

本研究を通して、高感度SIPが自然環境中に存在する未知のレアバイオスフィアの機能解明に極めて有効であることが明らかになった。高感度SIPは、今回対象とした研究対象のみならず、あらゆる自然環境試料に適用し得ると考えられる。よって本研究で得られた成果は、普遍的かつ強力な微生物生態系機能の評価・予測ツールを提供し、さらに生態系機能発現に関わる共通原理の理解に大きく貢献するものとして捉えられる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Remarks (1 results)

  • [Journal Article] Dead bacterial biomass-assimilating bacterial populations in compost revealed by high-sensitivity stable isotope probing2019

    • Author(s)
      Hanajima Dai、Aoyagi Tomo、Hori Tomoyuki
    • Journal Title

      Environment International

      Volume: 133(105235) Pages: 1-9

    • DOI

      10.1016/j.envint.2019.105235

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of the Wastewater Flow Rate on Interactions between the Genus <i>Nitrosomonas</i> and Diverse Populations in an Activated Sludge Microbiome2019

    • Author(s)
      Narihiro Takashi、Nobu Masaru Konishi、Hori Tomoyuki、Aoyagi Tomo、Sato Yuya、Inaba Tomohiro、Aizawa Hidenobu、Tamaki Hideyuki、Habe Hiroshi
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 1 Pages: 89-94

    • DOI

      10.1264/jsme2.ME18108

    • NAID

      130007627737

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of active and taxonomically diverse 1,4-dioxane degraders in a full-scale activated sludge system by high-sensitivity stable isotope probing2018

    • Author(s)
      Aoyagi Tomo、Morishita Fumiaki、Sugiyama Yutaka、Ichikawa Daisuke、Mayumi Daisuke、Kikuchi Yoshitomo、Ogata Atsushi、Muraoka Kenji、Habe Hiroshi、Hori Tomoyuki
    • Journal Title

      The ISME Journal

      Volume: 12 Issue: 10 Pages: 2376-2388

    • DOI

      10.1038/s41396-018-0201-2

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] High susceptibility of aerobic microbiota in membrane bioreactor (MBR) sludge towards olive oil as revealed by high-throughput sequencing of 16S rRNA genes2016

    • Author(s)
      Navarro R. Ronald、堀 知行、佐藤 由也、田中亮一、尾形 敦、羽部 浩
    • Journal Title

      Journal of Environmental Chemical Engineering

      Volume: 4 (4) Issue: 4 Pages: 4392-4399

    • DOI

      10.1016/j.jece.2016.09.037

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Identifying the elusive but key microbes in wastewater treatment systems by cutting-edge molecular ecological tools2019

    • Author(s)
      堀知行
    • Organizer
      The 59th seminar on water environments、清華大学(深センキャンパス)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] High-sensitivity stable isotope probing of elusive microbes that actively dissimilate but marginally assimilate substrate-13C in natural environments2019

    • Author(s)
      青柳 智、羽部 浩、堀 知行
    • Organizer
      8th IWA Microbial Ecology and Water Engineering Specialist Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高感度rRNA-SIPと動態解析で探る環境微生物の代謝様式2019

    • Author(s)
      青柳 智、羽部 浩、堀 知行
    • Organizer
      日本微生物生態学会第33回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 環境微生物の機能強化による新たな環境修復技術の開発2018

    • Author(s)
      堀知行
    • Organizer
      環境バイオテクノロジー学会2018年度大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 環境微生物群のモニタリング・機能強化による新たな環境バイオ技術の開発2018

    • Author(s)
      堀知行
    • Organizer
      日本農芸化学会 2019大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 有機性廃水を処理する活性汚泥中のパルミチン酸分解微生物群の同定2018

    • Author(s)
      青柳 智、堀 知行、田中亮一、Navarro Ronald、眞弓 大介、羽部 浩、柳下 宏、納崎克也、尾形 敦
    • Organizer
      日本水環境学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 超高感度安定同位体追跡法の適用による笹侵食湿地土壌におけるメタン生成抑制機構の解明2017

    • Author(s)
      青柳 智、Cuong Tu HO、成廣 隆、眞弓 大介、尾形 敦、羽部 浩、堀 知行
    • Organizer
      日本水環境学会シンポジウム
    • Related Report
      2017 Research-status Report
  • [Remarks] 環境微生物研究グループHP

    • URL

      https://unit.aist.go.jp/emri/114envmicrob/index.html

    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

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