Establishment of genome editing technology in a tropical crop, cassava, that feeds 1 billion people worldwide
Project/Area Number |
16K14832
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Science in genetics and breeding
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Seki Motoaki 国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (80281624)
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Project Period (FY) |
2016-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | キャッサバ / ゲノム編集 / 澱粉枝作り酵素 / CRISPR/Cas9 / CRISPR/Cas9システム / 澱粉 |
Outline of Final Research Achievements |
Cassava is a tropical starch crop that plays an important role in food security and improving the livelihood of poor farmers in the world's 1 billion people. In this study, we have targeted the starch branching enzyme gene by the introduction of the CRISPR/Cas9 system for establishing the basis of genome editing technology in cassava. A correlation between the genome editing pattern and the activity of the starch branching enzyme was observed in the plants produced by genome editing, showing the successful genome editing in the starch branching enzyme gene of cassava. In the future, the development of basic and applied research in cassava is expected by the use of the genome editing technology.
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Academic Significance and Societal Importance of the Research Achievements |
キャッサバは世界の10億人の、食糧安全保障上そして貧農の生活改善上重要な役割を果たしている熱帯のデンプン資源作物である。本研究では、CRISPR/Cas9システム(DNA二本鎖を切断してゲノム配列の任意の場所を削除、置換、挿入することができる画期的な新しい遺伝子改変技術)をキャッサバに導入し澱粉枝作り酵素遺伝子に対象を絞って研究を進め、キャッサバにおける植物ゲノム編集システムの基盤を確立した。今後、キャッサバにおいてゲノム編集技術の活用により基礎研究および応用研究の発展が期待される。
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Report
(5 results)
Research Products
(13 results)
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[Journal Article] Cassava breeding and agronomy in Asia: 50 years of history and future directions2020
Author(s)
Malik A.I, Kongsil P, Nguyen VA, Ou W, Sholihin, Srean P, Sheela MN, Lopez-Lavalle LAB, Utsumi Y, Lu C, Kittipadakul P, Nguyen HH, Ceballos H, Nguyen TH, Gomez MS, Aiemnaka P, Labarta R, Chen S, Amawan S, Sok S, Youabee L, Seki M, Tokunaga H, Wang W, Li K, Nguyen HA, Nguyen VD, Ham LH and Ishitani M
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Journal Title
Breeding Science
Volume: 70
Issue: 2
Pages: 145-166
DOI
NAID
ISSN
1344-7610, 1347-3735
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Advancement of Asian Cassava Molecular Breeding towards SDGs.2018
Author(s)
Seki M, Tokunaga H, Utsumi C, Okamoto Y, Moriya E, Vu TA, Sakamoto A, Takei Y, Sakurai T, Endo M, Mikami M, Toki S, Tsuji H, Narangajavana J, Triwitayakorn K, Sojikul P, Nguyen AH, Do QTN, Nguyen DV, Nguyen VA, Le HH, Pham NT, Nguyen HH, Touch B, Srean P, Wongtiem P, Ishitani M and Utsumi Y
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Journal Title
Proceedings of the 18th Science Council of Asia (SCA) Conference "Role of Science for Society: Strategies towards SDGs in Asia", Dec. 5-7, 2018, Tokyo, Japan
Volume: Theme 10
Pages: 6-6
Related Report
Int'l Joint Research
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[Journal Article] Formation of Friable Embryogenic Callus in Cassava is Enhanced under Conditions of Reduced Nitrate, Potassium and Phosphate2017
Author(s)
Utsumi, Y., Utsumi, C., Tanaka, M., Ha, T.V., Matsui, A., Takahashi, S. and Seki, M.
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Journal Title
PLoS ONE
Volume: 12
Issue: 8
Pages: e0180736-e0180736
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] 東南アジア諸国との連携による澱粉作物キャッサバの分子育種2017
Author(s)
内海好規, 徳永浩樹, 内海稚佳子, 櫻井哲也,Dong Van Nguyen, Vu Anh Nguyen, Jarunya Narangajavana, Ham Huy Le, 石谷学, 関原明
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Journal Title
応用糖質科学
Volume: 7
Pages: 143-146
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] The histone deacetylase inhibitor suberoylanilide hydroxamic acid alleviates salinity stress in cassava.2017
Author(s)
Patanun, O., Ueda, M., Itouga, M., Kato, Y., Utsumi, Y., Matsui, A., Tanaka, M., Utsumi, C., Sakakibara, H., Yoshida, M., Narangajavana, J., and Seki, M.
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Journal Title
Front. Plant Sci.
Volume: 7
Pages: 2039-2039
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Advancement of Asian Cassava Molecular Breeding by the use of genome editing technologies towards SDGs2019
Author(s)
Utsumi, Y., Tokunaga, H., Utsumi, C., Endo, M., Saika, H., Toki, S., Ishitani, M., Tsuji, H. and Seki, M.
Organizer
Inter-project Joint Open Seminar - New prospect of agriculture and bioindustry using genome editing technology -
Related Report
Int'l Joint Research / Invited
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[Presentation] Advancement of Asian Cassava Molecular Breeding towards SDGs.2018
Author(s)
Seki M, Tokunaga H, Utsumi C, Okamoto Y, Moriya E, Vu TA, Sakamoto A, Takei Y, Sakurai T, Endo M, Mikami M, Toki S, Tsuji H, Narangajavana J, Triwitayakorn K, Sojikul P, Nguyen AH, Do QTN, Nguyen DV, Nguyen VA, Le HH, Pham NT, Nguyen HH, Touch B, Srean P, Wongtiem P, Ishitani M and Utsumi Y
Organizer
The 18th Science Council of Asia (SCA) Conference “Role of Science for Society: Strategies towards SDGs in Asia"
Related Report
Int'l Joint Research / Invited
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[Presentation] 東南アジア諸国との連携による澱粉作物キャッサバの分子育種2016
Author(s)
内海好規,内海稚佳子, 武井良郎, 平野智也, 阿部知子, 石谷学, 櫻井哲也, Dong Van Nguyen, Vu Anh Nguyen, Kanokporn Triwitayakorn, Jarunya Narangajavana, Ham Huy Le, 関原明
Organizer
第5回応用糖質フレッシュシンポジウム
Place of Presentation
エフピコRiM7 階ものづくり交流館、福山、日本
Related Report
Invited
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