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Rapid exploration of a gene inducing salt-tolerance in barley by using a next-generation sequencer and analysis of its functions

Research Project

Project/Area Number 16K14834
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Crop production science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Hirasawa Tadashi  東京農工大学, (連合)農学研究科(研究院), 名誉教授 (30015119)

Research Collaborator KODAMA asuka  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsエクソームQTL-seq / 塩ストレス / オオムギ / 花粉 / 組換え自殖系統 / JAZ遺伝子 / 不稔 / 量的形質遺伝子座 / ジャスモン酸 / JAZタンパク質 / 遺伝子発現 / 作物学 / 植物生理学 / ゲノム / 稔実歩合 / 植物 / 生理学 / ストレス / 育種学
Outline of Final Research Achievements

Long-term salinity stress significantly reduces grain fertility in a barley cultivar, OUC613, but not in a cultivar OUE812, resulting in large differences in grain yield. Here, a gene affecting grain fertility under salt stress was explored by QTL-seq combined with exome-capture sequencing and the underlying causes of the difference in grain fertility between the cultivars was investigated.
A QTL for grain fertility was identified on chromosome 2H and the QTL region included a gene, MLOC_12120. MLOC_12120 was a gene encoding JAZ (jasmonate ZIM-domain) protein and expressed significantly at anther and pistil a few days before flowering in OUC613 compared with OUE812. Pollen fertility decreased significantly in OUC613 under salt stress, but not in OUE812. Salt stress decreased the number of pollen grains on stigma as well as pollen germination rate in OUC613 significantly compared with OUE812. MLOC_12120 might be a gene inducing the reduction in grain fertility under salt stress.

Academic Significance and Societal Importance of the Research Achievements

土壌の塩類集積は、世界の作物生産を制限する重要な問題となっている。塩ストレス耐性の機構についてこれまで多くの研究があるが、塩ストレス下での子実収量の低下機構と収量低下の鍵となる形質を解析した研究は少なく、したがって、収量差を引き起こす遺伝子とその作用機能を解明した研究もほとんどなかった。本研究によって耐塩性の最も高い作物の一つであるオオムギについて、塩ストレス下での子実重低下の品種間差を引き起こす候補遺伝子と子実重低下機構の一部を明らかにできた。研究成果は耐塩性作物の育成に向けた新たな知見を提供するものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley2018

    • Author(s)
      Kodama Asuka、Narita Ryouhei、Yamaguchi Makoto、Hisano Hiroshi、Adachi Shunsuke、Takagi Hiroki、Ookawa Taiichiro、Sato Kazuhiro、Hirasawa Tadashi
    • Journal Title

      Breeding Science

      Volume: 68 Issue: 5 Pages: 561-570

    • DOI

      10.1270/jsbbs.18082

    • NAID

      130007543984

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differences in dry matter production, grain production, and photosynthetic rate in barley cultivars under long-term salinity.2017

    • Author(s)
      Tadashi Hirasawa, Kosuke Sato, Makoto Yamaguchi, Ryohei Narita, Asuka Kodama, Shunsuke Adachi, Taiichiro Ookawa, Kazuhiro Sato
    • Journal Title

      Plant Production Science

      Volume: 20 Issue: 3 Pages: 288-299

    • DOI

      10.1080/1343943x.2017.1343647

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 塩ストレス下でのオオムギの花粉稔性低下に関わる遺伝子の探索2019

    • Author(s)
      児玉明日香・成田亮平・山口真功・久野裕・安達俊輔・高木宏樹・平沢正・佐藤和広・大川 泰一郎
    • Organizer
      日本育種学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 塩ストレス条件で稔実歩合を高く維持するオオムギの量的形質遺伝子座(QTL)の同定2018

    • Author(s)
      児玉 明日香・成田 亮平・山口 真功・久野 裕・安達 俊輔・高木 宏樹・大川 泰一郎・佐藤 和広・平沢 正
    • Organizer
      日本作物学会第245回講演会
    • Related Report
      2017 Research-status Report
  • [Presentation] QTLs maintaining pollen fertility under salt stress detected by exome QTL-seq and interval mapping in barley.2018

    • Author(s)
      Asuka Kodama, Ryouhei Narita, Makoto Yamaguchi, Hiroshi Hisano, Shunsuke Adachi, Hiroki Takagi, Taiichiro Ookawa, Kazuhiro Sato, Tadashi Hirasawa
    • Organizer
      アメリカ植物生物学会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Causes of varietal differences in grain fertility of barley under long-term salt stress2017

    • Author(s)
      Asuka Kodama, Ryohei Narita, Tammy L. Sage, Shaheen Bagha, Shunsuke Adachi, Taiichiro Ookawa, Kazuhiro Sato, Tadashi Hirasawa
    • Organizer
      第58回植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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